FastQCFastQC Report
Mon 6 Jun 2016
ERR522838_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522838_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9551007
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT925330.9688297788913777No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT274830.28774976293075694No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274780.2876974124299145No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC229980.2407913636750554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226220.23685460601170116No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC200980.21042807318641898No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA139320.14586943554747683No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC127690.13369270905151678No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126550.13249911763230832No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG382150.027.0894371
TACATGG392950.025.7525522
ACATGGG399100.024.881253
CATGGGG263450.023.4219594
ATGGGGG155500.021.1252175
GAGTACT256400.021.08552212-13
AGAGTAC450700.019.55245410-11
GTACTTT277750.019.3179214-15
AGTACTT271400.018.60368312-13
CATGGGA130900.017.0174854
ACTTTTT332800.016.30367516-17
GAGTACA304800.016.0021211
AGTACAT289300.015.791792
GTATAGG27700.015.7912661
TACTTTT323150.015.62934614-15
GTATAGA30800.015.57626151
TGGGGGG140600.015.3419936
GTATATA49450.014.9330031
TACCTGG58050.013.521592
GTATAAT44900.013.4084141
Produced by FastQC (version 0.11.5)