FastQCFastQC Report
Mon 6 Jun 2016
ERR522837_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522837_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9488722
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251020.2645456363881248No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206860.2180061761741992No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114230.12038502129159227No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA204450.034.954821
GTATCAA317200.032.5120051
TCAACGC363400.027.9730474
CAACGCA368450.027.5252885
ATCAACG370250.027.4442753
AACGCAG381500.026.6399776
TATCAAC384650.026.4449232
GTACATG315750.026.3436131
TACATGG324600.024.9220122
ACATGGG332150.023.7012733
ACGCAGA430900.023.5422527
CGCAGAG447250.022.670288
CATGGGG225000.021.3678724
GCAGAGT485700.020.5156279
GAGTACT243250.020.32904812-13
AGAGTAC377800.019.73381810-11
TATACCG7000.018.7983195
GTACTTT265050.018.54232814-15
ATGGGGG132100.017.9658435
CAGAGTA473250.017.79363610-11
Produced by FastQC (version 0.11.5)