FastQCFastQC Report
Mon 6 Jun 2016
ERR522837_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522837_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9488722
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234070.24668232455329603No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202740.21366417943322608No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112250.1182983335374353No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA215500.035.1036531
GTATCAA325950.031.4355051
TCAACGC374250.027.023014
ATCAACG381400.026.4802913
CAACGCA382450.026.3944665
TATCAAC397250.025.6327552
AACGCAG395150.025.592386
GTACATG325900.025.3052791
TACATGG333100.024.2012332
ACATGGG338450.023.06443
ACGCAGA445050.022.680677
CGCAGAG459500.021.9325288
CATGGGG231500.021.1326074
GAGTACT245600.020.2882812-13
GCAGAGT506350.019.8010039
AGAGTAC396800.019.19149610-11
ATGGGGG129000.018.8709325
GTACTTT261350.018.73930414-15
TACCTGG75200.018.5129322
CAGAGTA490400.017.53188910-11
Produced by FastQC (version 0.11.5)