FastQCFastQC Report
Mon 6 Jun 2016
ERR522836_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522836_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5586628
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134240.24028805927296393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90840.162602557392402No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG73900.043.6593932
GTATCAA156000.038.8011131
GGTATCA91000.037.3152241
ACCTGGG88300.035.8999063
GTACCTG91200.035.892581
TCAACGC182750.032.298074
CAACGCA186000.031.6811645
ATCAACG187350.031.5555463
TATCAAC191250.031.2320382
AACGCAG194650.030.4293256
TATAACG6200.030.3435482
ACGCAGA221700.026.608197
GTACATG204050.026.5525441
ATAACGC6950.025.7153783
TACATGG206950.025.5218622
CGCAGAG230650.025.3918368
ACATGGG210950.024.301953
TAACGCA6800.024.2074664
CATGGGG134950.023.489694
CCTGGGG85400.022.304244
Produced by FastQC (version 0.11.5)