FastQCFastQC Report
Mon 6 Jun 2016
ERR522836_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522836_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5586628
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130820.23416629852569384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91500.16378394981731378No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT69820.12497699864748467No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG78200.042.7967722
GTATCAA152600.037.8249051
GTACCTG92650.036.8112341
GGTATCA91800.036.6396371
ACCTGGG91000.036.1035773
TATAACG5900.032.6640172
TCAACGC181950.030.9212134
ATCAACG185750.030.3898543
CAACGCA186350.030.3175035
TATCAAC190050.029.8522572
AACGCAG196050.029.030916
ATAACGC7300.025.7544083
CCTGGGG84150.025.581494
ACGCAGA227850.024.9587947
GTACATG200750.024.3939971
CGCAGAG236600.023.9163598
TAACGCA7400.023.5008984
TACATGG206450.023.4281642
ACATGGG204400.022.6500823
CATGGGG132300.022.23974
Produced by FastQC (version 0.11.5)