FastQCFastQC Report
Mon 6 Jun 2016
ERR522835_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522835_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6863455
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275240.4010225170850541No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200390.29196665527784477No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135560.1975098547305985No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT92820.13523801059378987No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84450.12304298636765304No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG127400.057.104332
ACCTGGG140050.051.1069153
GTACCTG151550.048.934411
TATAACG8550.040.701812
CCTGGGG123700.036.9525764
ATAACGC9550.036.4398423
TAACGCA10450.034.2015274
CTGGGGG77500.027.0022585
GTATCAA346650.025.9379311
GTACATG246700.025.9243811
TATCACG6900.025.2174282
TACATGG253350.024.4834162
ACATGGG254150.023.3331363
CATGGGG150100.023.2785664
GGTATCA250750.023.0863741
TCAACGC394650.022.4261994
ATGGGGG92550.022.2556085
CAACGCA398050.022.2461325
ATCAACG398900.022.199053
TATCAAC403500.021.9925962
Produced by FastQC (version 0.11.5)