FastQCFastQC Report
Mon 6 Jun 2016
ERR522835_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522835_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6863455
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257500.3751754764910675No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201210.2931613888340493No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132490.19303688885553996No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT96280.14027920340411643No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71130.10363585104003742No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG134400.057.605022
ACCTGGG146550.051.6699143
GTACCTG162000.048.26161
CCTGGGG121350.040.4379124
TAACGCA9100.036.672884
ATAACGC9600.034.7628333
TATAACG9800.034.536032
TACCGGG9350.029.6624092
CTGGGGG75400.028.61365
TATCACG7200.027.4209672
GTACCGG11150.026.157881
GTACATG239350.025.452011
GGTATCA251800.024.5111581
GTATCAA345550.024.3956761
TACATGG245350.024.3706462
GTATAAC20750.024.2578011
ACATGGG243600.023.5595193
GTACCCG15000.023.2073841
CATGGGG149150.023.0683124
TGGGGGG104250.022.1351686
Produced by FastQC (version 0.11.5)