FastQCFastQC Report
Mon 6 Jun 2016
ERR522834_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522834_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7581602
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT610350.8050409398963438No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251150.3312624429507115No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198050.2612244747218332No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT164710.21724959975477479No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC128930.17005640760356452No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125220.1651629827047107No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC118170.1558641564144359No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA92300.12174208036771121No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG90800.11976360668892934No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA83710.11041202110055369No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC77500.10222114007039673No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG66500.033.2998542
GTACATG267350.030.0567721
TACATGG275500.028.4995462
GTACCTG81950.027.3110541
ACATGGG283750.027.1585313
CATGGGG193250.025.3507794
ACCTGGG87350.024.9219063
ATGGGGG119450.022.829355
GAGTACT218800.019.9910212-13
TAACGCA7550.019.9271934
ATAACGC6950.018.9424633
TATAACG6750.018.8062882
AGAGTAC328500.018.71282410-11
GAGTACA205450.018.4920771
TGGGGGG115400.018.1167816
GTACTTT242650.017.87174214-15
TATCACG6900.017.7160682
AGTACTT232000.017.52716412-13
AGTACAT197100.017.3893762
CATGGGA101550.017.0839984
Produced by FastQC (version 0.11.5)