FastQCFastQC Report
Mon 6 Jun 2016
ERR522834_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522834_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7581602
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT632480.8342300215706391No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235780.3109896826554599No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195450.25779512034527796No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT164840.21742106747360254No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC132010.17411887355732997No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC123900.16342192586738263No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120000.15827789430254977No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG100210.13217523156715427No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA98110.12940536841685968No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA87790.11579346950684037No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC80410.10605937900723356No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG63800.035.805752
GTACATG263200.029.7360041
TACATGG272050.028.1973722
GTACCTG85600.027.1436561
ACATGGG277850.026.7246653
ACCTGGG85550.025.7643053
CATGGGG188750.024.8242784
ATGGGGG115950.022.9004125
GAGTACT224050.019.32696312-13
AGAGTAC332500.018.86310210-11
TATCACG5550.018.6323172
TGGGGGG104250.018.3928786
GAGTACA202450.018.2608051
AGTACTT236700.017.61871712-13
AGTACAT194750.017.474242
GTACTTT246950.017.35984814-15
CCTGGGG86500.016.6254654
CATGGGA98650.016.1023064
TATACTG34350.016.0075455
TACTTTT282750.014.50517414-15
Produced by FastQC (version 0.11.5)