FastQCFastQC Report
Mon 6 Jun 2016
ERR522833_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522833_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8307203
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT528200.6358337457264497No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276590.3329520176646701No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220560.2655045266138314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122460.1474142379811833No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC110750.13331803737070105No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA95740.11524938056768325No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG92910.11184269843893305No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG291750.024.8159731
TACATGG293000.024.0499112
ACATGGG301950.022.7622623
GAGTACT240200.021.33115212-13
CATGGGG207400.021.0334644
AGAGTAC382350.019.56059310-11
GTACTTT260900.019.4602814-15
AGTACTT254700.018.253612-13
ATGGGGG119700.017.2791465
ACTTTTT313900.016.40552316-17
GAGTACA234650.016.3089241
TACCGTA5650.015.799217
GTATAGG22750.015.4989171
TACTTTT296650.015.420214-15
AGTACAT227800.015.39446452
GTATATA40700.015.0165591
CATGGGA104750.014.8091974
GTATATG34650.014.6535211
GTATTAG27250.014.3196721
GTGTAGC43700.014.0932561
Produced by FastQC (version 0.11.5)