FastQCFastQC Report
Mon 6 Jun 2016
ERR522833_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522833_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8307203
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT529440.63732642623516No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249510.30035380139380247No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210080.25288896876602146No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT158060.19026861387641544No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC126710.1525302800473276No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118220.142310233661077No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC115450.13897577800855476No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA95510.11497251240880955No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG93760.11286590685216191No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG292950.025.8341771
TACATGG297050.024.9689872
ACATGGG305550.023.5325973
CATGGGG207000.021.477394
GAGTACT238100.021.06682612-13
GTACTTT254200.019.39691214-15
AGAGTAC378000.019.21445710-11
ATGGGGG115500.018.9605795
AGTACTT250400.018.22967712-13
GAGTACA232850.016.362171
ACTTTTT308000.016.23531716-17
AGTACAT225400.015.8274652
TACTTTT294350.015.2979814-15
GTATAGC24400.015.0361571
CATGGGA109450.014.6419654
TATACTG38900.014.4970395
GTATAGG24300.013.9366481
GTATATA41450.013.617231
GTGTAGC47050.013.5960061
CTATACT28800.013.5439824
Produced by FastQC (version 0.11.5)