FastQCFastQC Report
Mon 6 Jun 2016
ERR522832_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522832_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8891120
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288970.3250096725721844No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230270.25898874382529985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131580.1479903544210403No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA146400.053.4819681
GTATCAA262800.041.628691
TCAACGC322400.033.4830254
CAACGCA329350.032.7477345
ATCAACG330700.032.7454573
TATCAAC331950.032.6981052
AACGCAG343400.031.5498856
TACCTGG79650.027.9745982
GTACATG338550.027.6443961
ACGCAGA392650.027.4368847
TACATGG341300.026.8164332
CGCAGAG406350.026.4761228
ACATGGG354400.025.0253943
CATGGGG271250.024.169254
GTGGTAT70550.023.7436831
GCAGAGT449200.023.489549
ATGGGGG174500.022.3262275
GTACCTG107100.022.274791
GAGTACT255900.021.24237412-13
TGGTATC75900.020.9956442
Produced by FastQC (version 0.11.5)