FastQCFastQC Report
Mon 6 Jun 2016
ERR522832_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522832_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8891120
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259580.2919542194909078No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221900.24957485671096555No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125090.1406909365749197No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140350.053.125161
GTATCAA248900.041.6930851
TCAACGC310650.032.9639474
TATCAAC318100.032.4381562
ATCAACG315750.032.4318773
CAACGCA316850.032.3485835
AACGCAG332700.031.047096
TACCTGG85400.028.3617212
GTACATG339600.027.925841
TACATGG345250.027.0265852
ACGCAGA383700.026.8101967
GTGGTAT63250.025.6591341
CGCAGAG399550.025.6451178
ACATGGG352850.025.5189973
CATGGGG275350.024.2698944
ATGGGGG173250.023.4365585
GTACCTG111000.023.1818311
ACCTGGG103150.023.0080783
GCAGAGT445650.022.7809499
TGGTATC70250.021.8919582
Produced by FastQC (version 0.11.5)