FastQCFastQC Report
Mon 6 Jun 2016
ERR522831_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522831_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5497843
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT473660.8615378794920118No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209820.3816405815880883No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162460.29549770700982186No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT133420.24267699168564835No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC106180.19313028764189882No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104900.19080210184248622No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC90970.16546489232231623No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA75040.136489892490564No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG71670.13036021581554802No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA69540.126485969133713No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC62360.1134263019151329No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG48450.034.5421522
ATAACGC5750.033.522853
TATAACG6000.030.5567042
GTACCTG58950.029.2700181
GTACATG181500.028.4424151
TAACGCA6300.028.3554294
TACATGG185300.027.094992
ACCTGGG61600.026.4833933
ACATGGG188050.025.925693
CATGGGG126650.025.0919654
ATGGGGG82400.023.5062315
GTATAAC15450.019.779971
AGAGTAC262600.019.67062410-11
GAGTACT183750.019.58640512-13
TATCACG4900.019.1878832
AGTACTT195850.018.13641512-13
TGGGGGG89600.017.72496
GTACTTT209300.017.02760314-15
CCTGGGG63350.016.696644
GTATACG3151.5087062E-716.41811
Produced by FastQC (version 0.11.5)