FastQCFastQC Report
Mon 6 Jun 2016
ERR522831_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522831_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5497843
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT488800.8890759521506889No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197460.3591590374625103No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162780.29607975345967497No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT134880.24533257861310337No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC110430.200860592054011No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100100.18207140509468894No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC95680.1740318885060923No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA81230.14774885350491093No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC64620.11753700496722078No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG50100.037.245222
GTACCTG59400.032.6989821
ACCTGGG63200.028.7028333
GTACATG177050.025.6597141
TACATGG177050.024.9279982
ACATGGG176350.024.223843
CATGGGG120050.023.1355514
ATGGGGG74400.021.9183125
TAACGCA5000.021.6178444
TATAACG5700.021.4395792
ATAACGC5400.020.8868053
TATCACG5650.020.7974132
GAGTACT186500.020.3163212-13
AGAGTAC261350.019.42612310-11
CCTGGGG59600.018.2934764
GTACTTT207400.018.01739114-15
AGTACTT198750.017.94061312-13
TGGGGGG79250.016.959676
GTATAAC15300.016.5986581
TATACTG27900.016.5071645
Produced by FastQC (version 0.11.5)