FastQCFastQC Report
Mon 6 Jun 2016
ERR522830_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522830_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11503133
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT427520.37165526991646536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350400.30461266508871976No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT286790.24931468670317905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216850.18851385965892944No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149120.1296342483391264No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA116960.10167664757070964No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG182450.050.0243072
GTACCTG213100.043.5249671
ACCTGGG214500.041.5852663
CCTGGGG201800.029.8224124
TATAACG10300.022.8261242
GTACATG547350.021.6632061
CATGGGG302150.021.5054
TATCACG12650.021.1876982
TACATGG560150.020.8351612
GAGTACT360700.020.52409212-13
ACATGGG555200.020.3433723
ATGGGGG162000.019.3278225
CTGGGGG129500.019.0595765
AGTACTT382350.018.63687112-13
GTATCAA822200.018.4544831
GTACACG25950.018.1249851
TAACGCA15300.017.8233994
GTACTTT419150.017.60739714-15
ATAACGC13500.017.415493
GTACCGG19900.016.7810941
Produced by FastQC (version 0.11.5)