FastQCFastQC Report
Mon 6 Jun 2016
ERR522830_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522830_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11503133
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT377280.32798021199963523No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT339940.29551949021192747No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT289900.25201829797151787No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212880.18506262598198248No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135330.11764620994993277No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG190200.051.2742082
GTACCTG218450.045.3731351
ACCTGGG220900.043.3985183
CCTGGGG205750.032.4331324
TATAACG11200.028.1156522
ATAACGC13150.026.4451713
CTGGGGG128050.022.2030415
TATCACG10250.021.55092
TAACGCA16050.021.0813244
GTACATG544000.020.527921
TACATGG556850.019.7585282
CATGGGG292000.019.5700154
GAGTACT377050.019.49060212-13
ACATGGG557400.019.0791053
GTATAAC27500.018.6369171
AGTACTT394500.017.95529612-13
TACCGTA9200.017.8779937
GTACACG26750.017.40171
GTATAGG32200.017.3768621
ATGGGGG156600.017.3149285
Produced by FastQC (version 0.11.5)