##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522830_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11503133
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17243424030653	33.0	31.0	34.0	30.0	34.0
2	32.305492686209924	34.0	31.0	34.0	30.0	34.0
3	32.494523100793494	34.0	31.0	34.0	30.0	35.0
4	35.593308275232495	37.0	35.0	37.0	33.0	37.0
5	35.52841039045624	36.0	35.0	37.0	33.0	37.0
6	35.65461148714876	37.0	35.0	37.0	33.0	37.0
7	35.49489656426645	36.0	35.0	37.0	33.0	37.0
8	35.48541688599097	36.0	35.0	37.0	33.0	37.0
9	36.76584440082541	37.0	37.0	39.0	33.0	39.0
10-11	36.569802026978216	37.5	35.0	39.0	33.0	39.0
12-13	36.57693451862201	37.5	35.0	39.0	33.0	39.0
14-15	37.735404389395484	38.0	37.0	41.0	33.0	41.0
16-17	37.55713856390254	38.0	36.5	41.0	32.5	41.0
18-19	37.4531703232502	38.0	36.0	41.0	32.0	41.0
20-21	37.468150633397	38.0	36.0	41.0	33.0	41.0
22-23	37.31502195966959	38.0	36.0	40.5	32.5	41.0
24-25	37.2328321771121	38.0	36.5	40.0	32.0	41.0
26-27	36.91658020471466	38.0	36.0	40.0	32.0	41.0
28-29	36.87448276047925	38.0	36.0	40.0	32.0	41.0
30-31	36.63626800629011	38.0	35.5	40.0	31.0	41.0
32-33	36.588193103565786	37.5	36.0	40.0	30.5	41.0
34-35	36.34924880899838	37.5	35.0	40.0	30.5	41.0
36-37	36.11734972550522	37.0	35.0	40.0	30.0	41.0
38-39	36.069061967726526	37.0	35.0	40.0	30.0	41.0
40-41	36.2626769593988	37.5	35.0	40.0	30.0	41.0
42-43	36.30052021479713	37.5	35.0	40.0	30.5	41.0
44-45	36.122846271533156	37.0	35.0	40.0	30.0	41.0
46-47	36.01510931847871	37.0	35.5	40.0	29.5	41.0
48-49	35.78749193806592	37.5	35.0	40.0	29.5	41.0
50-51	35.61399485687943	37.5	34.5	40.0	28.5	41.0
52-53	35.614520713617765	37.5	35.0	40.0	28.5	41.0
54-55	35.41200158252539	37.5	34.0	40.0	27.0	41.0
56-57	35.15797291920384	37.0	34.5	40.0	27.5	41.0
58-59	34.96164405818832	37.0	34.0	39.5	27.0	41.0
60-61	34.76175451505255	36.5	34.0	39.0	26.5	41.0
62-63	34.52337576206413	36.5	34.0	39.0	26.0	41.0
64-65	34.329637369228024	36.5	33.0	38.5	25.5	40.0
66-67	33.82496933661464	35.5	33.0	38.0	26.0	40.0
68-69	33.683308451706154	35.5	33.0	37.0	25.0	40.0
70-71	33.262158665817395	35.0	32.5	37.0	25.0	39.0
72-73	33.032801324647814	35.0	32.5	37.0	24.5	39.0
74-75	32.84060759794745	35.0	32.0	37.0	23.5	39.0
76-77	31.27685687890421	34.0	29.5	36.0	22.0	36.5
78-79	32.394476270073554	35.0	31.5	36.5	23.0	37.0
80-81	32.50356737594879	35.0	32.0	36.0	24.0	37.5
82-83	32.412587857586274	35.0	32.0	37.0	23.5	37.0
84-85	32.08489265489671	35.0	32.0	35.5	23.0	36.5
86-87	31.971965724468284	35.0	32.0	36.0	23.0	36.0
88-89	31.917469918847324	35.0	31.5	36.0	23.0	37.0
90-91	31.67899475734133	34.5	31.0	35.0	23.0	36.5
92-93	31.470737102665858	34.0	31.0	35.0	21.5	36.5
94-95	31.59457632107705	34.0	31.0	35.0	21.0	36.5
96-97	31.319030563238726	34.0	31.0	35.0	21.0	37.0
98-99	31.168192265533225	34.0	31.0	35.0	20.0	36.0
100	29.92947356168098	34.0	29.0	35.0	18.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.0757873530069304
1101	2	-0.12040950393594585
1101	3	-0.10895707157966683
1101	4	-0.12315643414448374
1101	5	-0.21485746687958596
1101	6	-0.1252969719459216
1101	7	-0.13310071168264415
1101	8	-0.2253967232321159
1101	9	-0.13703207435491294
1101	10-11	-0.17771498034888822
1101	12-13	-0.1234068614630317
1101	14-15	-0.33483453856182877
1101	16-17	-0.36012238901454907
1101	18-19	-0.4410043632541303
1101	20-21	-0.3822033497345245
1101	22-23	-0.9029575713447784
1101	24-25	-0.3985932561679135
1101	26-27	-0.3244546161425106
1101	28-29	-0.38835369919038243
1101	30-31	-0.4010305906333187
1101	32-33	-0.3812232706303078
1101	34-35	-0.3719563869841451
1101	36-37	-0.46754443678732116
1101	38-39	-0.3453440450178391
1101	40-41	-0.39922649909571106
1101	42-43	-0.38637993664680437
1101	44-45	-0.48854850397726324
1101	46-47	-0.4139252539673848
1101	48-49	-0.5017249541972149
1101	50-51	-0.49399541426225824
1101	52-53	-0.5573648887547407
1101	54-55	-0.5246044674848207
1101	56-57	-0.5455922702573943
1101	58-59	-0.5608627994082553
1101	60-61	-0.766019784873599
1101	62-63	-0.6193846324809016
1101	64-65	-0.580695822919779
1101	66-67	-0.488675054878307
1101	68-69	-0.4869346155280141
1101	70-71	-0.6254003018626761
1101	72-73	-0.702953563777875
1101	74-75	-0.6012167451864556
1101	76-77	-0.682332568051077
1101	78-79	-0.5841394604873997
1101	80-81	-0.4670410570672985
1101	82-83	-0.443567315438294
1101	84-85	-0.5125864226122836
1101	86-87	-0.5806509042470687
1101	88-89	-0.6032976055725676
1101	90-91	-0.5995983804475351
1101	92-93	-0.5976910496690984
1101	94-95	-0.6296451365684099
1101	96-97	-0.7393479183602025
1101	98-99	-0.6860686350109653
1101	100	-0.6895745076476913
1102	1	-0.05963206080917871
1102	2	-0.11972667773907375
1102	3	-0.1580387082831436
1102	4	-0.1336051517274086
1102	5	-0.16130431877741813
1102	6	-0.20745686301479083
1102	7	-0.16892569697607485
1102	8	-0.2096702589292434
1102	9	-0.2012276760612437
1102	10-11	-0.31182346574832565
1102	12-13	-0.19257365453834296
1102	14-15	-0.32289149199815625
1102	16-17	-0.3387272570153783
1102	18-19	-0.3084558306406606
1102	20-21	-0.3747880713839251
1102	22-23	-0.29366048883554896
1102	24-25	-0.2667493799349785
1102	26-27	-0.29261404304759253
1102	28-29	-0.3665297549733211
1102	30-31	-0.37018474493373077
1102	32-33	-0.29753354223886674
1102	34-35	-0.28167362175187804
1102	36-37	-0.41175404367345436
1102	38-39	-0.4510041170303225
1102	40-41	-0.3699486484687853
1102	42-43	-0.43485565054005804
1102	44-45	-0.3198480663326393
1102	46-47	-0.3448789619381287
1102	48-49	-0.4387143591350977
1102	50-51	-0.2946177916224073
1102	52-53	-0.4066134475863734
1102	54-55	-0.3105015746884092
1102	56-57	-0.3315473038728953
1102	58-59	-0.33499550698375913
1102	60-61	-0.4975907281576397
1102	62-63	-0.5468868825967093
1102	64-65	-0.4918277162960507
1102	66-67	-0.5476784669399279
1102	68-69	-0.4001242682623882
1102	70-71	-0.4864753332934342
1102	72-73	-0.5413263486295463
1102	74-75	-0.5885720914133259
1102	76-77	-0.4897667779131858
1102	78-79	-0.5746224096795238
1102	80-81	-0.5535166147551998
1102	82-83	-0.46384697600300484
1102	84-85	-0.5045652371284319
1102	86-87	-0.5061165040477071
1102	88-89	-0.45438863190442547
1102	90-91	-0.5953560977349177
1102	92-93	-0.5573686859729996
1102	94-95	-0.6356166049808429
1102	96-97	-0.7217588259709729
1102	98-99	-0.8465661597111342
1102	100	-0.862234485974593
1103	1	-0.08263227301954856
1103	2	-0.10795296948975164
1103	3	-0.24306397676917157
1103	4	-0.041170977936396014
1103	5	-0.12308990224781269
1103	6	-0.15630142892982235
1103	7	-0.1526998828309729
1103	8	-0.17531139014916164
1103	9	-0.14364166991087757
1103	10-11	-0.2814238976194261
1103	12-13	-0.19932329953599748
1103	14-15	-0.22769486063378253
1103	16-17	-0.3002251607106672
1103	18-19	-0.4068113653825023
1103	20-21	-0.2859019534957241
1103	22-23	-0.33677370054275
1103	24-25	-0.2785045504328423
1103	26-27	-0.2850317700942142
1103	28-29	-0.32153015273751606
1103	30-31	-0.48764196989372977
1103	32-33	-0.3025660849289551
1103	34-35	-0.3983366487251274
1103	36-37	-0.39370969454352434
1103	38-39	-0.287376951012682
1103	40-41	-0.3642881584825943
1103	42-43	-0.3814169633635771
1103	44-45	-0.2733611922388164
1103	46-47	-0.4202571260570025
1103	48-49	-0.38019484535859505
1103	50-51	-0.4378195012567119
1103	52-53	-0.36838204012157405
1103	54-55	-1.1074374142081922
1103	56-57	-0.4450641426190032
1103	58-59	-0.46908822799177585
1103	60-61	-0.43585366021888916
1103	62-63	-0.4052848334450445
1103	64-65	-0.3824486791957469
1103	66-67	-0.40207888352560417
1103	68-69	-0.3778347139383058
1103	70-71	-0.46630918502928154
1103	72-73	-0.49499787092613445
1103	74-75	-0.5631787901344083
1103	76-77	-0.468425085349633
1103	78-79	-0.3730167566863898
1103	80-81	-1.1968566442267772
1103	82-83	-0.20155409850845984
1103	84-85	-0.22526870929176113
1103	86-87	-0.24423969608596963
1103	88-89	-0.3383155790900432
1103	90-91	-0.3217963107134061
1103	92-93	-0.2725944890098795
1103	94-95	-0.34312459629052583
1103	96-97	-0.4508820398570208
1103	98-99	-0.33288333555738703
1103	100	-0.3889469370136496
1104	1	-0.0627281979755665
1104	2	-0.18265197559478707
1104	3	-0.14237279604953557
1104	4	-0.01213751976330002
1104	5	-0.08692865656563953
1104	6	-0.1972751938677746
1104	7	-0.1889416829457602
1104	8	-0.10636675583707245
1104	9	-0.1908497310964421
1104	10-11	-0.18677767366569498
1104	12-13	-0.18371478126483964
1104	14-15	-0.2836878858631309
1104	16-17	-0.2711160168231288
1104	18-19	-0.29306037477720537
1104	20-21	-0.3066738010458181
1104	22-23	-0.24608350684145108
1104	24-25	-0.18974532612958228
1104	26-27	-0.24977285488103007
1104	28-29	-0.3377999740940396
1104	30-31	-0.33037005600586156
1104	32-33	-0.2541001695371605
1104	34-35	-0.30355625084744275
1104	36-37	-0.47911583860195606
1104	38-39	-0.24400769326931737
1104	40-41	-0.22373188950636802
1104	42-43	-0.2595098762006529
1104	44-45	-0.33425606279820386
1104	46-47	-0.2835850767210033
1104	48-49	-0.35618603549851713
1104	50-51	-0.33104367080104424
1104	52-53	-0.38624058582249887
1104	54-55	-0.32160775208046744
1104	56-57	-0.34262425743744274
1104	58-59	-0.31802309911299176
1104	60-61	-0.4146732099672974
1104	62-63	-0.3410056751509458
1104	64-65	-0.519797587668478
1104	66-67	-0.4655363055279622
1104	68-69	-0.3305760935985447
1104	70-71	-0.30305619195795686
1104	72-73	-0.32759597482232294
1104	74-75	-0.431943906659761
1104	76-77	-0.37960796900643956
1104	78-79	-0.32737284176099024
1104	80-81	-0.3264595691396295
1104	82-83	-0.3444489380193332
1104	84-85	-0.9182212965695342
1104	86-87	-0.36322281393884737
1104	88-89	-0.40847006005992625
1104	90-91	-0.4368355244029374
1104	92-93	-0.38102962233839577
1104	94-95	-0.3440692627480786
1104	96-97	-0.27268566695746443
1104	98-99	-0.2775711796987572
1104	100	-1.499936120407039
1105	1	-0.01099133830033594
1105	2	-0.12811491566843358
1105	3	-0.15098570188809646
1105	4	-0.11003665855723455
1105	5	-0.13945562984103788
1105	6	-0.10679922743767634
1105	7	-0.10641677883273815
1105	8	-0.16675584830723267
1105	9	-0.1448444806686382
1105	10-11	-0.1741185923666535
1105	12-13	-0.14017399813332077
1105	14-15	-0.19451931587361315
1105	16-17	-0.181749234166233
1105	18-19	-0.2775029115265468
1105	20-21	-0.22154743660319554
1105	22-23	-0.23457623936413796
1105	24-25	-0.1474097839477011
1105	26-27	-0.25112440110266476
1105	28-29	-0.1686274648957422
1105	30-31	-0.2546653664942724
1105	32-33	-0.17741728013916003
1105	34-35	-0.28780330812185895
1105	36-37	-0.3371532411116931
1105	38-39	-0.2535187183938916
1105	40-41	-0.2207799718745065
1105	42-43	-0.23803197305752377
1105	44-45	-0.3510856546146428
1105	46-47	-0.3818157265597222
1105	48-49	-0.3631195011891819
1105	50-51	-0.2932109100476197
1105	52-53	-0.4536583560023715
1105	54-55	-1.294935527172946
1105	56-57	-0.3614989517831759
1105	58-59	-0.3008631554355219
1105	60-61	-0.30221824348058135
1105	62-63	-0.2084563010179039
1105	64-65	-0.21773074055786878
1105	66-67	-0.282199923941981
1105	68-69	-0.14912452152749722
1105	70-71	-0.1075391036588158
1105	72-73	-0.18834105223417197
1105	74-75	-0.21217765966827784
1105	76-77	-0.19974143920667586
1105	78-79	-0.18639982664373633
1105	80-81	-0.1308941866867599
1105	82-83	-0.10005949591765528
1105	84-85	-0.13009651455617188
1105	86-87	-0.165037513384366
1105	88-89	-0.25305059534725416
1105	90-91	-0.3187794208089123
1105	92-93	-0.14942760527493348
1105	94-95	-0.23672111322171574
1105	96-97	-0.23769653901550925
1105	98-99	-0.15728321705514858
1105	100	-0.17452174633315565
1106	1	0.035863174153540456
1106	2	-0.08823157847241703
1106	3	-0.07513119007945335
1106	4	-0.014631304197372685
1106	5	-0.10811930801987302
1106	6	-0.053162239267322775
1106	7	-0.052059696743690154
1106	8	-0.10420925528259772
1106	9	-0.10716745175528075
1106	10-11	-0.12237189490051747
1106	12-13	-0.13900595980140196
1106	14-15	-0.14508078111978762
1106	16-17	-0.10475161762134633
1106	18-19	-0.1376569486075212
1106	20-21	-0.1726149017512313
1106	22-23	-0.09268313299175901
1106	24-25	-0.041482436850635906
1106	26-27	-0.032200540929778754
1106	28-29	-0.048178817794486406
1106	30-31	-0.1196894279517906
1106	32-33	0.06098334384289217
1106	34-35	0.020626837360367745
1106	36-37	-0.08771200406614099
1106	38-39	-0.06052633541533936
1106	40-41	-0.07571108125605974
1106	42-43	-0.12449966420256686
1106	44-45	-0.07123464844189442
1106	46-47	-0.1654369059393801
1106	48-49	-0.10129663688837809
1106	50-51	-0.034751492554796926
1106	52-53	-0.09900241488880113
1106	54-55	-0.13097611181552793
1106	56-57	-0.05798476502811667
1106	58-59	-0.08660140909262282
1106	60-61	-0.06743407393877021
1106	62-63	-0.14871223339319783
1106	64-65	-0.05874269703890178
1106	66-67	0.014848757159917625
1106	68-69	0.08891918179632796
1106	70-71	0.08567000035127137
1106	72-73	0.033746985695870535
1106	74-75	-0.037671556099184045
1106	76-77	-0.05824968230256644
1106	78-79	0.049007198866178214
1106	80-81	0.1048269053438986
1106	82-83	-0.014179066995900769
1106	84-85	0.09955979910188972
1106	86-87	0.12108039766639322
1106	88-89	0.003014789850375621
1106	90-91	0.10280981308877202
1106	92-93	0.16534882394473627
1106	94-95	0.15070846473316024
1106	96-97	0.18477535645916987
1106	98-99	0.026608242194367193
1106	100	0.20567106671382263
1107	1	-0.0280742515916117
1107	2	-0.08681680840926731
1107	3	-0.1082462578435397
1107	4	-0.0581359284595635
1107	5	-0.12644286173843255
1107	6	-0.15810100604790733
1107	7	-0.12843411933668136
1107	8	-0.1776953309873477
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2316	98-99	0.3547000627790915
2316	100	0.08673335704264318
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	1.0
10	3.0
11	9.0
12	83.0
13	434.0
14	1604.0
15	4141.0
16	9052.0
17	16860.0
18	27451.0
19	38844.0
20	51929.0
21	68328.0
22	86680.0
23	100635.0
24	112982.0
25	128083.0
26	147413.0
27	175323.0
28	212052.0
29	256861.0
30	314692.0
31	388455.0
32	484146.0
33	628310.0
34	886097.0
35	1576977.0
36	2280967.0
37	1719216.0
38	1517131.0
39	268251.0
40	120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.42813045532314	17.821620978498416	12.799157832043711	23.951090734134727
2	16.730715042788706	20.35601165468598	38.46468908882929	24.44858421369602
3	19.856790319645963	25.33189001639814	29.22761129511412	25.583708368841773
4	13.4622238959751	16.114875895976493	36.09635565811349	34.32654454993492
5	14.098263917594961	36.30010319842081	33.497758818222906	16.103874065761325
6	31.730827743261575	34.88205646949022	18.154386127186925	15.232729660061278
7	28.5098142853293	31.483717909821067	21.496044989494166	18.510422815355472
8	27.018058136375984	33.50679054604727	20.029270306981797	19.44588101059495
9	27.17093682216836	15.775241405971746	20.03218601401896	37.02163575784093
10-11	25.834314008192376	25.031358848063395	27.680580586175957	21.453746557568273
12-13	26.38671701133885	22.916531920427737	27.704591734544	22.99215933368941
14-15	23.618277422735225	24.33735433132616	25.85196555035005	26.192402695588555
16-17	23.112206655785982	26.92766136420262	26.538248349076245	23.42188363093515
18-19	22.907841620276017	26.231716220803104	28.203654023973396	22.656788134947487
20-21	23.708541975724582	25.282752539536197	28.1661697036404	22.842535781098825
22-23	23.48853544685699	25.142068657353494	27.958496279035195	23.410899616754318
24-25	23.411521835573414	25.386422530014663	27.917709073290986	23.28434656112094
26-27	23.288373176618723	25.14793178511549	28.28563371119248	23.278061327073303
28-29	23.096178796527724	25.653344914252724	28.15556252555334	23.094913763666213
30-31	23.644357248208152	25.333316292856416	27.966826364625952	23.055500094309476
32-33	23.399884614963298	25.498367378236708	27.98181468530192	23.119933321498078
34-35	23.713250524708396	25.43877845642662	27.910804658939327	22.93716635992566
36-37	23.722140380359633	25.335971592348994	27.90842192612324	23.033466101168134
38-39	23.578938912713564	25.652245673360785	27.740227296051913	23.028588117873745
40-41	23.527550279221543	25.6056914509566	27.740581081615627	23.12617718820623
42-43	23.3084057264162	25.724351396448057	27.438416161278944	23.528826715856795
44-45	23.387699947341154	25.620628009550405	27.436819165768085	23.55485287734036
46-47	23.564327743673942	25.6789780720318	27.336191459252706	23.42050272504155
48-49	23.56810336099116	25.782249521582763	27.24510921920315	23.40453789822293
50-51	23.15112519225036	26.109096214429716	27.275453451742194	23.46432514157773
52-53	23.408373133577673	26.21157505158813	26.746059798632725	23.63399201620147
54-55	23.17936753317002	25.97694838066799	27.025892863818306	23.817791222343683
56-57	23.48053723809156	25.939824131480965	26.793516191525384	23.786122438902087
58-59	23.634672857264015	25.85158207173308	26.93176758273232	23.581977488270585
60-61	23.605586997519136	25.983258411327764	27.167682168533698	23.2434724226194
62-63	23.285840400621918	26.095653220888426	27.101337333409255	23.517169045080397
64-65	23.194316489833938	26.089996155385037	26.957731620664948	23.75795573411608
66-67	23.287960793284935	25.940079524267933	26.74827965971921	24.023680022727927
68-69	23.08928058524603	26.03104998918555	26.787270384194628	24.092399041373792
70-71	23.20995761626123	25.8975340982653	26.786723717302824	24.105784568170645
72-73	23.16502602397503	26.031799241894888	26.73658235978787	24.066592374342207
74-75	23.058812892294057	26.430432988095365	26.438948563360725	24.07180555624985
76-77	23.027000339448936	26.03828057225813	26.65855623226549	24.276162856027444
78-79	23.055819684191743	26.095199760578108	26.676606355503697	24.172374199726455
80-81	23.186798231489348	26.24929198590418	26.388506323781307	24.17540345882517
82-83	23.097363200339668	26.20304856556539	26.533770511413245	24.165817722681698
84-85	22.979028742813405	26.44360740449	26.29356526150795	24.283798591188646
86-87	22.929887938757297	26.429379596864667	26.33796064273193	24.302771821646107
88-89	22.724776365825733	26.262077662419074	26.327512166171125	24.685633805584065
90-91	23.06533587474063	26.199914970565914	26.26361967284315	24.47112948185031
92-93	23.04210059438056	26.347405752969554	26.216214665513583	24.3942789871363
94-95	22.812519098064534	26.153644803948563	26.361770784279653	24.672065313707254
96-97	22.605546047803273	26.132415025297114	26.553995890077747	24.708043036821863
98-99	22.782377150810294	26.149567365776683	26.23567475747251	24.832380725940514
100	22.66939392840877	25.934449222571317	26.29399537325244	25.10216147576747
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3893.0
1	3022.0
2	5895.5
3	8268.5
4	5035.5
5	3565.0
6	4951.0
7	8854.5
8	13502.5
9	15514.0
10	13510.0
11	10789.5
12	10715.5
13	11561.0
14	12619.5
15	13496.5
16	14396.0
17	15524.5
18	16693.5
19	17947.5
20	19241.0
21	21014.0
22	23652.0
23	27173.0
24	31456.0
25	37283.5
26	45221.5
27	54055.5
28	64071.0
29	75968.5
30	90280.0
31	104883.5
32	120114.5
33	137195.0
34	153736.0
35	170848.5
36	195011.5
37	219769.0
38	236632.5
39	253162.0
40	268050.5
41	281700.5
42	298890.0
43	320376.5
44	341965.5
45	366015.0
46	393294.5
47	431581.5
48	493266.5
49	516779.5
50	507559.5
51	493573.5
52	468472.0
53	460219.0
54	455527.0
55	436301.0
56	403284.0
57	368656.5
58	339076.0
59	305577.0
60	260577.5
61	216064.5
62	178619.5
63	141237.0
64	107027.0
65	81283.0
66	61282.5
67	47962.5
68	37944.0
69	29332.5
70	23409.0
71	18607.5
72	14795.0
73	12164.5
74	9853.5
75	7503.5
76	5443.5
77	3641.5
78	2434.5
79	1688.5
80	1169.0
81	766.0
82	516.5
83	359.5
84	237.0
85	152.0
86	107.0
87	76.0
88	46.0
89	29.0
90	24.5
91	14.5
92	7.0
93	6.5
94	5.0
95	1.5
96	1.0
97	0.5
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04510944974729928
2	0.009997276394178873
3	0.0
4	3.5642463666202934E-4
5	4.346641910512553E-4
6	8.693283821025108E-6
7	2.607985146307532E-5
8	6.954627056820086E-5
9	0.0
10-11	0.0
12-13	2.521052308097281E-4
14-15	0.011331695460706228
16-17	0.001556097803963494
18-19	5.6506344836663196E-5
20-21	8.475951725499479E-4
22-23	5.911432998297072E-4
24-25	0.004381415045796653
26-27	0.01566095080357673
28-29	0.012653074601502043
30-31	0.009627811831785306
32-33	0.006737294961294457
34-35	0.008954082335655861
36-37	0.0051985837249730145
38-39	0.004081496753971288
40-41	0.005372449401393516
42-43	0.007176305794256226
44-45	0.006885080786251885
46-47	0.014404771291438601
48-49	0.016756304565025894
50-51	0.016117348204180548
52-53	0.010557993200634991
54-55	0.003712032191577721
56-57	0.003903284435640273
58-59	0.010201568563972963
60-61	0.00245150603752908
62-63	1.564791087784519E-4
64-65	2.6514515654126573E-4
66-67	8.997548754760985E-4
68-69	4.346641910512554E-5
70-71	1.608257506889645E-4
72-73	0.0032556347909739026
74-75	0.005915779640207585
76-77	0.0057940736667132335
78-79	0.0036989922658461826
80-81	6.519962865768831E-5
82-83	0.001012767565149425
84-85	2.7383844036229084E-4
86-87	4.1727762340920514E-4
88-89	2.3906530507819047E-4
90-91	4.998638197089436E-4
92-93	5.65063448366632E-4
94-95	3.477313528410043E-5
96-97	0.0020776948332250004
98-99	4.8247725206689344E-4
100	1.7386567642050215E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.1503133E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.31796531961084
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.98405902150014	31.695493817461095
2	17.70944829346603	16.75950120359334
3	6.641059519677964	9.4272427211278
4	3.04517397301792	5.76365745789774
5	1.6288317476782201	3.8536502074059573
6	0.9472793204607923	2.6893998020128977
7	0.6410635053516291	2.123367449972758
8	0.42980493682703064	1.6269996075983173
9	0.318123753442611	1.354767185946771
>10	1.5014369955459619	12.524075891048255
>50	0.09596023493160104	3.1281976318115494
>100	0.05339941045176444	4.6273128219055355
>500	0.002906277301211738	0.9382357576263286
>1k	0.001075227660851714	0.8704879761925916
>5k	2.0342144652622996E-4	0.6027343674753126
>10k+	1.7436123987962568E-4	2.0148761009237353
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37728	0.32798021199963523	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33994	0.29551949021192747	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	28990	0.25201829797151787	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21288	0.18506262598198248	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13533	0.11764620994993277	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.693283821025106E-6
2	0.0	0.0	0.0	0.0	8.693283821025106E-6
3	0.0	0.0	0.0	0.0	8.693283821025106E-6
4	0.0	0.0	0.0	0.0	5.215970292615064E-5
5	0.0	8.693283821025106E-6	0.0	0.0	5.215970292615064E-5
6	8.693283821025106E-6	8.693283821025106E-6	0.0	0.0	5.215970292615064E-5
7	8.693283821025106E-6	8.693283821025106E-6	0.0	0.0	5.215970292615064E-5
8	8.693283821025106E-6	8.693283821025106E-6	0.0	0.0	5.215970292615064E-5
9	8.693283821025106E-6	8.693283821025106E-6	0.0	8.693283821025106E-6	9.562612203127618E-5
10-11	8.693283821025106E-6	8.693283821025106E-6	0.0	8.693283821025106E-6	9.562612203127618E-5
12-13	8.693283821025106E-6	8.693283821025106E-6	0.0	1.7386567642050212E-5	9.997276394178873E-5
14-15	8.693283821025106E-6	8.693283821025106E-6	0.0	1.7386567642050212E-5	1.5647910877845193E-4
16-17	8.693283821025106E-6	8.693283821025106E-6	0.0	3.4773135284100424E-5	2.0863881170460257E-4
18-19	8.693283821025106E-6	8.693283821025106E-6	0.0	5.6506344836663196E-5	2.0863881170460257E-4
20-21	8.693283821025106E-6	1.7386567642050212E-5	0.0	1.0431940585230127E-4	2.1298545361511512E-4
22-23	8.693283821025106E-6	2.607985146307532E-5	0.0	2.4341194698870302E-4	2.564518727202407E-4
24-25	8.693283821025106E-6	2.607985146307532E-5	0.0	7.69355618160722E-4	3.390380690199792E-4
26-27	8.693283821025106E-6	2.607985146307532E-5	0.0	0.0016778037774578458	3.390380690199792E-4
28-29	8.693283821025106E-6	2.607985146307532E-5	0.0	0.004346641910512553	3.390380690199792E-4
30-31	8.693283821025106E-6	2.607985146307532E-5	0.0	0.012766087291175367	3.477313528410043E-4
32-33	8.693283821025106E-6	2.607985146307532E-5	0.0	0.03343436957566256	3.738112043040796E-4
34-35	8.693283821025106E-6	2.607985146307532E-5	0.0	0.06645580816982644	4.0858433958818004E-4
36-37	8.693283821025106E-6	3.477313528410043E-5	0.0	0.1181417271277312	4.0858433958818004E-4
38-39	8.693283821025106E-6	4.346641910512554E-5	0.0	0.19600312367074257	4.4335747487228046E-4
40-41	8.693283821025106E-6	4.346641910512554E-5	0.0	0.28651324817334545	4.4335747487228046E-4
42-43	8.693283821025106E-6	4.346641910512554E-5	0.0	0.37379381773643755	4.563974006038181E-4
44-45	8.693283821025106E-6	4.346641910512554E-5	0.0	0.48212952071405246	4.694373263353558E-4
46-47	8.693283821025106E-6	4.346641910512554E-5	0.0	0.6161060643217808	5.215970292615064E-4
48-49	8.693283821025106E-6	4.346641910512554E-5	0.0	0.7507780706351913	5.476768807245817E-4
50-51	8.693283821025106E-6	4.346641910512554E-5	0.0	0.8922395316128223	5.476768807245817E-4
52-53	8.693283821025106E-6	4.346641910512554E-5	0.0	1.0359395131743674	5.694100902771445E-4
54-55	8.693283821025106E-6	4.346641910512554E-5	0.0	1.1999165792484534	6.041832255612449E-4
56-57	8.693283821025106E-6	4.346641910512554E-5	0.0	1.3921337778151397	6.172231512927826E-4
58-59	8.693283821025106E-6	4.346641910512554E-5	0.0	1.601959222761312	6.172231512927826E-4
60-61	1.3039925731537659E-5	5.215970292615064E-5	0.0	1.8219558097780841	6.172231512927826E-4
62-63	1.7386567642050212E-5	5.215970292615064E-5	0.0	2.0485201727216404	6.519962865768831E-4
64-65	1.7386567642050212E-5	5.215970292615064E-5	0.0	2.280948155602478	6.606895703979082E-4
66-67	1.7386567642050212E-5	6.954627056820085E-5	0.0	2.54411124343255	6.737294961294458E-4
68-69	1.7386567642050212E-5	6.954627056820085E-5	0.0	2.8469243987703177	6.780761380399584E-4
70-71	1.7386567642050212E-5	6.954627056820085E-5	0.0	3.1726878233955915	6.954627056820086E-4
72-73	1.7386567642050212E-5	6.954627056820085E-5	0.0	3.486758781281586	8.171686791763601E-4
74-75	1.7386567642050212E-5	6.954627056820085E-5	0.0	3.807775672940581	8.171686791763601E-4
76-77	1.7386567642050212E-5	6.954627056820085E-5	0.0	4.1568675246995745	8.215153210868727E-4
78-79	1.7386567642050212E-5	6.954627056820085E-5	0.0	4.55208594041293	8.258619629973852E-4
80-81	1.7386567642050212E-5	6.954627056820085E-5	0.0	4.970724062740125	8.258619629973852E-4
82-83	1.7386567642050212E-5	6.954627056820085E-5	0.0	5.399589833482756	8.345552468184103E-4
84-85	1.7386567642050212E-5	6.954627056820085E-5	0.0	5.821787855534661	8.345552468184103E-4
86-87	1.7386567642050212E-5	6.954627056820085E-5	0.0	6.261524577695486	8.389018887289229E-4
88	1.7386567642050212E-5	6.954627056820085E-5	0.0	6.615597681083927	8.519418144604605E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	19020	0.0	51.274208	2
GTACCTG	21845	0.0	45.373135	1
ACCTGGG	22090	0.0	43.398518	3
CCTGGGG	20575	0.0	32.433132	4
TATAACG	1120	0.0	28.115652	2
ATAACGC	1315	0.0	26.445171	3
CTGGGGG	12805	0.0	22.203041	5
TATCACG	1025	0.0	21.5509	2
TAACGCA	1605	0.0	21.081324	4
GTACATG	54400	0.0	20.52792	1
TACATGG	55685	0.0	19.758528	2
CATGGGG	29200	0.0	19.570015	4
GAGTACT	37705	0.0	19.490602	12-13
ACATGGG	55740	0.0	19.079105	3
GTATAAC	2750	0.0	18.636917	1
AGTACTT	39450	0.0	17.955296	12-13
TACCGTA	920	0.0	17.877993	7
GTACACG	2675	0.0	17.4017	1
GTATAGG	3220	0.0	17.376862	1
ATGGGGG	15660	0.0	17.314928	5
>>END_MODULE