FastQCFastQC Report
Mon 6 Jun 2016
ERR522829_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522829_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8653871
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT349480.40384239607916506No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277050.32014574749265384No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176630.20410519176909386No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148820.17196928403485562No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG152100.054.747992
GTACCTG176100.047.912931
ACCTGGG178450.046.1637843
CCTGGGG160400.035.1453974
TATAACG8600.032.8043632
GTATCAA416650.027.373481
ATAACGC10700.026.8058683
CTGGGGG100650.025.9240365
GTACCCG16750.025.8322751
GGTATCA296700.025.7746411
TATCACG9750.025.0771142
TAACGCA12400.023.508434
TCAACGC484150.023.4137484
CAACGCA488400.023.1602555
GTACATG373800.023.1131691
ATCAACG489700.023.0443783
TATCAAC496300.022.785032
AACGCAG500400.022.7460786
CATGGGG223500.022.6774854
TACATGG380900.022.3432542
Produced by FastQC (version 0.11.5)