FastQCFastQC Report
Mon 6 Jun 2016
ERR522829_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522829_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8653871
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302550.34961232955748933No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263230.30417601556575086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170900.19748387744628967No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126360.14601558077304364No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG157100.055.3454972
GTACCTG179200.049.2762451
ACCTGGG180900.046.9928253
CCTGGGG162100.036.4984364
TATAACG9100.032.537632
ATAACGC10950.028.3244423
TAACGCA10650.026.4748334
CTGGGGG99150.026.3520015
GGTATCA304700.025.5082381
GTATCAA414150.025.2838131
TATCACG7800.024.101952
GTACATG362700.023.1403921
CATGGGG215100.022.7426934
TGGGGGG134150.022.594526
TACATGG374450.021.7641662
TCAACGC482700.021.4179024
CAACGCA489150.021.145095
ATCAACG489150.021.1258773
ACATGGG375200.021.0791363
TATCAAC495100.020.9980492
Produced by FastQC (version 0.11.5)