FastQCFastQC Report
Mon 6 Jun 2016
ERR522828_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522828_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6600342
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191550.2902122344569418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153090.23194252661452996No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89640.1358111443316119No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107850.043.6154981
GTATCAA185200.038.6067851
TACCTGG66900.034.356362
TCAACGC219500.031.9209064
ATCAACG224250.031.226183
CAACGCA223950.031.222725
TATCAAC231700.030.5508542
GTACATG221800.030.1789671
AACGCAG233100.030.1123586
TACATGG227900.028.688462
ACCTGGG80150.028.379643
GTACCTG83500.028.1672551
ACATGGG229600.027.5510543
ACGCAGA265850.026.2616427
CATGGGG165950.025.9105074
CGCAGAG276400.025.240538
ATGGGGG104000.024.3090425
GCAGAGT304550.022.4425189
TATCACG6300.021.6362442
GAGTACT177250.020.10309412-13
Produced by FastQC (version 0.11.5)