FastQCFastQC Report
Mon 6 Jun 2016
ERR522828_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522828_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6600342
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173470.2628197144935823No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146910.22257937543236397No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84430.1279176139660642No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107950.045.538931
GTATCAA185850.037.816141
TACCTGG69200.037.036622
TCAACGC218650.031.5718924
ATCAACG224250.030.7844073
CAACGCA224000.030.7758755
GTACCTG86150.030.52431
TATCAAC231300.030.0293432
ACCTGGG83900.029.8542883
AACGCAG232800.029.7536016
GTACATG217600.028.7745361
TACATGG224150.027.6304512
ACGCAGA263450.026.292047
ACATGGG228350.026.1980463
CGCAGAG275400.025.1383178
CATGGGG163950.024.5925854
ATGGGGG99500.024.4171945
GCAGAGT309250.022.2803339
TGGGGGG94650.020.4551016
GAGTACT174650.020.45277812-13
Produced by FastQC (version 0.11.5)