FastQCFastQC Report
Mon 6 Jun 2016
ERR522827_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522827_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10926256
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250890.22962119869788883No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196370.1797230451126168No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109730.10042781351635913No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA150400.049.7108541
GTATCAA266600.041.6158521
TCAACGC321400.033.8962944
ATCAACG330950.032.9929663
CAACGCA329850.032.9420055
TATCAAC345000.031.748642
AACGCAG343500.031.7195636
GTACATG376700.028.0786271
ACGCAGA397900.027.3005267
TACATGG389300.026.553952
CGCAGAG414100.026.1977448
ACATGGG401200.025.2359263
GCAGAGT465750.023.0590119
CATGGGG272650.022.5813794
GAGTACT243550.020.63150412-13
CAGAGTA446250.020.060110-11
AGAGTAC413700.019.53761510-11
GTACTTT260250.019.11725614-15
ATGGGGG147900.017.6361395
AGTACTT266950.017.11582812-13
Produced by FastQC (version 0.11.5)