FastQCFastQC Report
Mon 6 Jun 2016
ERR522827_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522827_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10926256
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225550.20642935695447737No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188710.17271240944748137No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA152650.049.8308071
GTATCAA268900.040.4640661
TCAACGC327200.032.5870174
CAACGCA336250.031.6959765
ATCAACG337100.031.6175023
AACGCAG350000.030.4769336
TATCAAC354450.030.2363152
ACGCAGA406900.026.1918897
GTACATG380300.026.1371291
CGCAGAG424100.025.1371158
TACATGG392950.024.9042972
ACATGGG400050.023.6233273
GCAGAGT481350.022.0692799
CATGGGG271200.020.8102234
GAGTACT247350.019.90649212-13
CAGAGTA459500.019.3905410-11
AGAGTAC429450.019.31895810-11
GTACTTT263250.018.44258914-15
GTGGTAT90250.017.9306471
AGTACTT265750.017.36079412-13
Produced by FastQC (version 0.11.5)