FastQCFastQC Report
Mon 6 Jun 2016
ERR522826_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522826_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11736720
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT299080.25482417574927235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239070.20369404739995503No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124350.10594953274850215No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA163800.054.2918551
GTATCAA310200.043.715851
TCAACGC372150.035.8762744
CAACGCA379350.035.095045
ATCAACG381650.034.9626463
TATCAAC392000.034.2247852
AACGCAG396400.033.743146
ACGCAGA456800.029.1477787
CGCAGAG476350.027.8810468
GTACATG417100.025.6190911
GCAGAGT528900.024.7186629
TACATGG426850.024.6135962
ACATGGG438500.023.4092353
CATGGGG298600.021.719184
GAGTACT286650.021.61895612-13
CAGAGTA512650.020.88349210-11
GTGGTAT89500.020.556091
GTACTTT307300.020.01314214-15
AGAGTAC482900.019.95208210-11
TGGTATC90850.019.4034582
Produced by FastQC (version 0.11.5)