FastQCFastQC Report
Mon 6 Jun 2016
ERR522826_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522826_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11736720
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268730.22896516232814618No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230620.19649442092850472No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117410.10003646674709799No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161650.053.9593161
GTATCAA302400.043.1886981
TCAACGC365250.035.227614
ATCAACG369800.034.7826543
CAACGCA371800.034.5817265
TATCAAC384750.033.5682262
AACGCAG388100.033.1526836
ACGCAGA452050.028.4209827
CGCAGAG472150.027.1587548
GTACATG412750.024.5864321
GCAGAGT531900.023.7631729
TACATGG418650.023.6487122
ACATGGG428450.022.551623
GTGGTAT83900.021.3646321
CATGGGG298150.020.8843564
GAGTACT285150.020.54854212-13
CAGAGTA514150.020.23247710-11
AGAGTAC482600.019.91394410-11
TGGTATC84750.019.4793242
GTACTTT297050.019.43794414-15
Produced by FastQC (version 0.11.5)