##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522826_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11736720
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.692765014416295	34.0	31.0	34.0	30.0	35.0
2	32.750917036446296	34.0	31.0	34.0	31.0	34.0
3	33.03100576651739	34.0	31.0	34.0	31.0	36.0
4	35.81083726969715	37.0	35.0	37.0	33.0	37.0
5	35.86246702656279	37.0	35.0	37.0	35.0	37.0
6	35.93413585737753	37.0	35.0	37.0	35.0	37.0
7	35.759723500262425	37.0	35.0	37.0	35.0	37.0
8	35.8104236106851	37.0	36.0	37.0	34.0	37.0
9	37.0297644486705	39.0	36.0	39.0	34.0	39.0
10-11	37.15717159478969	39.0	36.0	39.0	34.0	39.0
12-13	37.026927242023326	38.0	36.5	39.0	34.0	39.0
14-15	38.132981872277774	39.0	36.5	41.0	34.5	41.0
16-17	38.15368965946193	39.0	37.0	41.0	34.5	41.0
18-19	38.1554742721987	39.0	37.0	41.0	34.0	41.0
20-21	38.04377905411393	39.0	36.5	41.0	34.0	41.0
22-23	37.964527866388565	39.0	36.5	41.0	34.5	41.0
24-25	37.83631031497727	39.0	36.0	41.0	34.0	41.0
26-27	37.7149251238847	39.0	36.5	41.0	34.0	41.0
28-29	37.680679227245776	39.0	36.5	41.0	34.5	41.0
30-31	37.53733462159786	38.5	36.0	41.0	34.0	41.0
32-33	37.409931778214016	38.0	36.5	41.0	33.0	41.0
34-35	37.24631869040073	38.0	36.0	41.0	33.0	41.0
36-37	37.27361946097376	38.0	36.0	41.0	33.0	41.0
38-39	37.42025020619049	38.0	36.5	41.0	33.0	41.0
40-41	37.3241843973444	38.0	36.5	41.0	33.0	41.0
42-43	37.362761955640075	38.0	36.5	41.0	33.5	41.0
44-45	37.2099713122576	38.0	36.0	41.0	33.0	41.0
46-47	36.9612400653675	38.0	36.0	41.0	32.5	41.0
48-49	36.937348168824	38.0	36.5	41.0	32.5	41.0
50-51	36.55173515258096	38.0	35.0	40.5	31.0	41.0
52-53	36.6092155644848	38.0	36.0	40.0	31.0	41.0
54-55	36.41422484305666	37.5	35.5	40.0	31.0	41.0
56-57	36.12584201548644	38.0	35.5	40.0	30.0	41.0
58-59	35.998827483317314	37.0	35.0	40.0	30.0	41.0
60-61	35.734846447729865	36.5	35.0	40.0	30.0	41.0
62-63	35.56008906236154	37.5	35.0	40.0	29.0	41.0
64-65	35.421525562508094	37.0	35.0	39.0	29.0	41.0
66-67	35.20471213422489	36.5	34.5	39.0	29.0	41.0
68-69	34.737862707809334	36.0	34.0	38.5	27.5	40.0
70-71	34.49765360339175	36.0	34.0	38.0	28.0	40.0
72-73	34.23362395967527	36.0	34.0	37.0	27.0	39.5
74-75	33.955251722798195	35.5	34.0	37.5	26.5	39.0
76-77	32.4670422400807	34.5	31.0	36.5	25.0	37.5
78-79	33.46575887471116	35.0	33.0	37.0	26.5	38.0
80-81	33.45287482363045	35.0	34.0	36.5	27.0	37.0
82-83	33.27093101820611	35.0	33.5	36.0	26.5	37.5
84-85	33.06959440116148	35.0	33.0	36.0	26.0	37.0
86-87	32.82188712008125	35.0	33.0	36.0	26.0	37.0
88-89	32.65769013830099	35.0	33.0	36.0	25.5	36.5
90-91	32.51866927046057	35.0	33.0	35.5	25.0	37.0
92-93	32.369796714925464	35.0	32.5	35.5	25.0	36.5
94-95	32.24844398605403	35.0	32.0	35.0	25.0	36.0
96-97	32.075587600283555	35.0	32.0	35.0	24.0	36.5
98-99	31.950116429462405	35.0	32.0	35.0	24.0	36.5
100	30.76641105862626	34.0	29.0	35.0	19.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.09588557361292516
1101	2	-0.13105080273362546
1101	3	-0.14216926084690584
1101	4	-0.09571019127031377
1101	5	-0.10983522317745553
1101	6	-0.16358696743858303
1101	7	-0.11851311027879774
1101	8	-0.09514621877592333
1101	9	-0.10314516282399921
1101	10-11	-0.08356880530246258
1101	12-13	-0.124919559977819
1101	14-15	-0.2453098053782341
1101	16-17	-0.07526925529114692
1101	18-19	-0.13528650045078194
1101	20-21	-0.1514403477419961
1101	22-23	-0.08305968309272771
1101	24-25	-0.20630410044754655
1101	26-27	-0.2211843145515786
1101	28-29	-0.23552624061697003
1101	30-31	-0.22674594534076675
1101	32-33	-0.16287238968354245
1101	34-35	-0.15195328669817343
1101	36-37	-0.2668892419391753
1101	38-39	-0.1639767591392669
1101	40-41	-0.2114651532445393
1101	42-43	-0.23245353064577046
1101	44-45	-0.25450145753777065
1101	46-47	-0.31178127239763853
1101	48-49	-0.3936008354729452
1101	50-51	-0.26547613148879634
1101	52-53	-0.34926539244222
1101	54-55	-0.26355831261267326
1101	56-57	-0.39837582805004246
1101	58-59	-0.4817531851916428
1101	60-61	-0.49345782241802993
1101	62-63	-0.37312211813907936
1101	64-65	-0.3875913321691158
1101	66-67	-0.4681946159296828
1101	68-69	-0.45211337766927073
1101	70-71	-0.43083843436500047
1101	72-73	-0.47580549237419945
1101	74-75	-0.4353405043696412
1101	76-77	-0.43616500828528615
1101	78-79	-0.3865737254135624
1101	80-81	-0.29473821769786923
1101	82-83	-0.38633501486949484
1101	84-85	-0.42572226229248855
1101	86-87	-0.4568715360435789
1101	88-89	-0.441618587572961
1101	90-91	-0.4324977557166889
1101	92-93	-0.7300143486573205
1101	94-95	-0.6091579471553672
1101	96-97	-0.5159254865949592
1101	98-99	-0.5243800295607635
1101	100	-0.5862968602476428
1102	1	-0.032313391551547
1102	2	-0.08841187071959666
1102	3	-0.09116212139736746
1102	4	-0.058491891543390295
1102	5	-0.07973267966378472
1102	6	-0.1214802244612514
1102	7	-0.1282667551113974
1102	8	-0.056294183039760526
1102	9	-0.1158260657812491
1102	10-11	-0.1049803457806675
1102	12-13	-0.11808011602810353
1102	14-15	-0.23160956757428863
1102	16-17	-0.08152221917766411
1102	18-19	-0.14779292800484
1102	20-21	-0.1521955427694337
1102	22-23	-0.11943339733018377
1102	24-25	-0.1918406266949475
1102	26-27	-0.14837206633014688
1102	28-29	-0.13062977273451537
1102	30-31	-0.11013928620818092
1102	32-33	-0.13758181922298007
1102	34-35	-0.1844562525097757
1102	36-37	-0.2415027542467314
1102	38-39	-0.25638777916403654
1102	40-41	-0.2904181330470479
1102	42-43	-0.2399194149273498
1102	44-45	-0.32225298243997713
1102	46-47	-0.25575190387324653
1102	48-49	-0.20901962323491574
1102	50-51	-0.11443430871769067
1102	52-53	-0.2630835319193636
1102	54-55	-0.3354511377653111
1102	56-57	-0.16332515188408792
1102	58-59	-0.296645107510237
1102	60-61	-0.29753713797600057
1102	62-63	-0.20585957676017586
1102	64-65	-0.25113746934610504
1102	66-67	-0.2379314309512921
1102	68-69	-0.2596325859936286
1102	70-71	-0.2501695523332259
1102	72-73	-0.42339963245883894
1102	74-75	-0.80943534171913
1102	76-77	-0.35687534637477825
1102	78-79	-0.30385158600608975
1102	80-81	-0.1980721398596188
1102	82-83	-0.2171669787781738
1102	84-85	-0.2665198781401017
1102	86-87	-0.34203294665275763
1102	88-89	-0.1987186210456997
1102	90-91	-0.13773677572771703
1102	92-93	-0.29388104611741284
1102	94-95	-0.335733103646799
1102	96-97	-0.3561746094385576
1102	98-99	-0.5643987652033466
1102	100	-0.3523710837848988
1103	1	-0.10769784027868923
1103	2	-0.11827588009506229
1103	3	-0.1045951209360183
1103	4	-0.041906260674167584
1103	5	-0.14116473853742662
1103	6	-0.07784253794819307
1103	7	-0.09597997903425437
1103	8	-0.07659801807413658
1103	9	-0.1463062916594211
1103	10-11	-0.07393667018921235
1103	12-13	-0.08242143428352477
1103	14-15	-0.15856076347034076
1103	16-17	-0.1422181441008945
1103	18-19	-0.11867361546514132
1103	20-21	-0.15864274701960568
1103	22-23	-0.10212211843246166
1103	24-25	-0.14589424771276782
1103	26-27	-0.24454240237288616
1103	28-29	-0.19441343484114526
1103	30-31	-0.18044323165969445
1103	32-33	-0.11783029214668517
1103	34-35	-0.09398519288357932
1103	36-37	-0.1082738362409188
1103	38-39	-0.14621248656309405
1103	40-41	-0.20451455945078578
1103	42-43	-0.24099987246509613
1103	44-45	-0.32776543159326366
1103	46-47	-0.26880606150348996
1103	48-49	-0.2707706468091047
1103	50-51	-0.12394557716357468
1103	52-53	-0.17594976700770815
1103	54-55	-0.1998094017151928
1103	56-57	-0.020449026385257696
1103	58-59	-0.1409072466062966
1103	60-61	-0.2032755065023082
1103	62-63	-0.13117244554597107
1103	64-65	-0.19736065962271
1103	66-67	-0.20404338790207532
1103	68-69	-0.122237733483054
1103	70-71	-0.29492816537315036
1103	72-73	-0.35705895420777267
1103	74-75	-0.24331185544161826
1103	76-77	-0.18417512840395034
1103	78-79	-0.2284972018621474
1103	80-81	-0.18710052708066627
1103	82-83	-0.20467631532448394
1103	84-85	-0.13854841982655586
1103	86-87	-0.2222223256632745
1103	88-89	-0.20972611568609523
1103	90-91	-0.202442042918193
1103	92-93	-0.2078283538187904
1103	94-95	-0.004301542317186602
1103	96-97	-0.7810225924718459
1103	98-99	-1.1551200752467032
1103	100	-0.3632218639137754
1104	1	-0.017402103354669407
1104	2	-0.038852211585108876
1104	3	-0.08922867982091987
1104	4	-0.06386452087880912
1104	5	-0.13783896085565317
1104	6	-0.11977922122434137
1104	7	-0.11397650204150267
1104	8	-0.03525044581354564
1104	9	-0.021197350042008622
1104	10-11	-0.10188148993184853
1104	12-13	-0.059848154423143285
1104	14-15	-0.13660466601605492
1104	16-17	-0.022977431849305674
1104	18-19	-0.13061696622336427
1104	20-21	-0.20093780357563418
1104	22-23	-0.13102043380742145
1104	24-25	-0.12242857269043128
1104	26-27	-0.031195231430032777
1104	28-29	-0.10273445824308425
1104	30-31	-0.206788969242524
1104	32-33	-0.11525760632172677
1104	34-35	-0.0681604341344979
1104	36-37	-0.14902660778886911
1104	38-39	-0.10874787193171187
1104	40-41	-0.11018955206304781
1104	42-43	-0.15176758761982967
1104	44-45	-0.24544791437566715
1104	46-47	-0.12086606236503883
1104	48-49	-0.14815815129376375
1104	50-51	-0.07025491133236983
1104	52-53	-0.08119904162124669
1104	54-55	-0.15691825788732672
1104	56-57	-0.11809151536620988
1104	58-59	-0.12480710075041657
1104	60-61	-0.07788943338129428
1104	62-63	-0.0421248041261606
1104	64-65	-0.2037879655016681
1104	66-67	-0.25022124681293434
1104	68-69	-0.04124277692815781
1104	70-71	-0.12677827341973114
1104	72-73	-0.10572652253060255
1104	74-75	-0.19289364163208234
1104	76-77	-0.22091132871464936
1104	78-79	-0.2149297919502331
1104	80-81	-0.05100110923982726
1104	82-83	-0.05110908375629464
1104	84-85	-0.08917805302009896
1104	86-87	-0.13299332376213613
1104	88-89	-0.16981102726154518
1104	90-91	-0.1321562497657709
1104	92-93	-0.20445119140671153
1104	94-95	-0.24689718346353118
1104	96-97	0.0457180344897381
1104	98-99	-0.8482038613490488
1104	100	-0.23794356230677138
1105	1	-0.11326918958189225
1105	2	-1.0153990840475302
1105	3	-0.10922979273445321
1105	4	-0.07518593226755854
1105	5	-0.1097442779721689
1105	6	-0.058805198778784984
1105	7	-0.12352006238784696
1105	8	-0.11762979568118226
1105	9	-0.15333184368138575
1105	10-11	-0.0744965835579805
1105	12-13	-0.06623825641246839
1105	14-15	-0.1148816200824001
1105	16-17	-0.6033379047164189
1105	18-19	-0.08635089587615852
1105	20-21	-0.19135223662615175
1105	22-23	-0.14508946486193963
1105	24-25	-0.15717989686798006
1105	26-27	-0.14121280186833474
1105	28-29	-0.17909521835866116
1105	30-31	-0.1500086350608214
1105	32-33	-0.1553512204770513
1105	34-35	-0.1428310385280369
1105	36-37	-0.19758490107975746
1105	38-39	-0.2082292799355301
1105	40-41	-0.15229800160847873
1105	42-43	-0.18991503018681044
1105	44-45	-0.33597573910947887
1105	46-47	-0.2643346913849882
1105	48-49	-0.18862753717335323
1105	50-51	-0.14414171565084644
1105	52-53	-0.24154304999153453
1105	54-55	-0.2969189346119592
1105	56-57	-0.15907149499300743
1105	58-59	-0.2672560783057705
1105	60-61	-0.180241408800633
1105	62-63	-0.11409773705813109
1105	64-65	-0.20257911002168072
1105	66-67	-0.1825175290586145
1105	68-69	-0.21633714907607526
1105	70-71	-0.21713092538939804
1105	72-73	-0.2706638265860306
1105	74-75	-0.2336206774522509
1105	76-77	-0.16761805460365764
1105	78-79	-0.18044511601571145
1105	80-81	-0.10648971858726242
1105	82-83	-0.054394575316123905
1105	84-85	-0.039859501217790694
1105	86-87	-0.12246376298779893
1105	88-89	-0.17235191474168232
1105	90-91	-0.18273283179043176
1105	92-93	-0.18527592874669807
1105	94-95	-0.20887711154072974
1105	96-97	-0.1599157917719083
1105	98-99	-0.14432707891715424
1105	100	-0.10893344050199971
1106	1	-0.08813358077627242
1106	2	-0.112131057672336
1106	3	-0.05354965813915413
1106	4	-0.10371320826759955
1106	5	-0.08594676835749482
1106	6	-0.09361711469394862
1106	7	-0.06941227946271766
1106	8	-0.045638924264224556
1106	9	-0.13915820675502744
1106	10-11	-0.13320440874843342
1106	12-13	-0.06582593119104274
1106	14-15	-0.10545829203109491
1106	16-17	-0.02662577899547358
1106	18-19	-0.10163734557327331
1106	20-21	-0.10807479929379582
1106	22-23	-0.02619623065008625
1106	24-25	-0.03508464463457983
1106	26-27	-0.0012924567908214613
1106	28-29	-0.0778372008292223
1106	30-31	-0.050091327093944926
1106	32-33	0.0027991655288204242
1106	34-35	-0.0345320542803762
1106	36-37	-0.07818673020120315
1106	38-39	-0.12718700167581432
1106	40-41	-0.11223118063480086
1106	42-43	-0.1408684173482726
1106	44-45	-0.1674643341688835
1106	46-47	-0.1779954016375953
1106	48-49	-0.19969142704018594
1106	50-51	-0.0939461988165533
1106	52-53	-0.20694541153799406
1106	54-55	-0.21841904481504315
1106	56-57	-0.1413769207298614
1106	58-59	-0.24451043495925973
1106	60-61	-0.13952335685514328
1106	62-63	-0.10857839000514247
1106	64-65	-0.09633846054306616
1106	66-67	-0.12190833187953842
1106	68-69	-0.07419594522261974
1106	70-71	-0.06394955200252639
1106	72-73	-0.15224091324333244
1106	74-75	-0.14873003045916278
1106	76-77	-0.21493296960882446
1106	78-79	-0.11471005693164926
1106	80-81	-0.008075303706597481
1106	82-83	-0.03812707986781305
1106	84-85	0.07083727960382902
1106	86-87	0.020564626732962665
1106	88-89	-0.05266365213012136
1106	90-91	-0.038983412030646036
1106	92-93	-0.043590177993607426
1106	94-95	-0.08079956852237302
1106	96-97	-0.15326694826867993
1106	98-99	-0.16434658633775712
1106	100	-0.10665606150577744
1107	1	-0.017345133647978628
1107	2	-0.08289600503098171
1107	3	-0.06050279415761395
1107	4	-0.043413776214450195
1107	5	-0.047052019019695024
1107	6	-0.08460191519541382
1107	7	-0.06730994577790028
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2316	80-81	-0.10953462228969357
2316	82-83	-0.08187234436874036
2316	84-85	-0.009467212841180128
2316	86-87	-0.15530134120719197
2316	88-89	-0.08037311609015774
2316	90-91	-0.1300572846445931
2316	92-93	-0.1662756631013167
2316	94-95	-0.124488189633432
2316	96-97	-0.01291620077806499
2316	98-99	-5.07917592674545E-4
2316	100	-0.11492826435453196
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	1.0
11	12.0
12	48.0
13	190.0
14	736.0
15	2050.0
16	4568.0
17	8717.0
18	14696.0
19	22288.0
20	32325.0
21	46015.0
22	61665.0
23	72272.0
24	78716.0
25	86589.0
26	99435.0
27	119612.0
28	147309.0
29	180831.0
30	225959.0
31	284704.0
32	365466.0
33	493635.0
34	745148.0
35	1431428.0
36	2561157.0
37	1944923.0
38	2219598.0
39	486413.0
40	213.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.11338570839029	19.157584215413088	13.903501437731622	23.825528638465002
2	15.844975964928768	20.704311919236293	39.53414920861712	23.91656290721782
3	18.742655318708046	25.581090393770346	30.024835588201615	25.651418699319994
4	13.114675879466775	17.245712897153325	37.907211507411695	31.732399715968203
5	13.859310115893503	37.4935958613395	34.575677300767495	14.071416721999505
6	31.053710523131873	36.905250404290165	17.990988887771778	14.050050184806187
7	27.470843642857627	32.47264141940849	22.08093061775351	17.97558431998037
8	25.87358474538219	34.96872068216288	20.439427916046707	18.71826665640822
9	26.490770755766903	15.969001204255296	20.23128449751779	37.30894354246001
10-11	24.80879451914969	26.100407618176767	28.40486340347758	20.685934459195966
12-13	25.50721298721537	23.70819543447072	28.307750429070357	22.47684114924355
14-15	22.994133448359914	25.18786015663822	25.92646340087012	25.891542994131743
16-17	22.622188638759116	28.132781083510878	26.498538140933785	22.746492136796217
18-19	22.497457122111474	27.26071600011656	28.262858680402726	21.978968197369245
20-21	23.352485949936227	26.40499892197038	28.226236348313012	22.016278779780382
22-23	23.134251801516942	26.25901269697315	27.971687125069167	22.635048376440743
24-25	22.84731598441681	26.432491667214453	28.085757046863957	22.634435301504784
26-27	22.74336737508822	26.603383270850195	28.07129362147934	22.581955732582244
28-29	22.713363566658064	26.607421927174425	28.10293236664406	22.576282139523457
30-31	23.080196829585706	26.4401381019566	28.02862588467971	22.451039183777986
32-33	22.826488047472083	26.56778737242244	27.931405280258954	22.674319299846523
34-35	23.043269097224293	26.628894303104705	27.92408553309756	22.403751066573438
36-37	23.071447602816267	26.540417378599006	27.839644022609946	22.54849099597478
38-39	22.88868283395544	26.6650775869249	27.866140694052945	22.580098885066718
40-41	22.999112668896668	26.475432925385967	27.924729033415762	22.600725372301603
42-43	22.859724784024714	26.5176166239283	27.80235773305359	22.82030085899339
44-45	22.836066616510877	26.66219726579516	27.745325437978774	22.75641067971518
46-47	22.890336713631562	26.565719175033898	27.52106364286073	23.022880468473808
48-49	22.86317440746872	26.681394481189884	27.509700893588352	22.94573021775304
50-51	22.903348515446975	26.810598921302713	27.3481836213998	22.93786894185051
52-53	22.951757743325647	26.80961984655414	27.277640754032927	22.960981656087288
54-55	22.81391523388082	26.684273164507267	27.415201844217595	23.08660975739432
56-57	22.810486207576133	26.879738407163284	27.21003540521388	23.099739980046703
58-59	22.819809004139266	26.743518870384225	27.362875094364703	23.07379703111181
60-61	22.932179491117513	26.788889485801114	27.35571941624484	22.923211606836535
62-63	22.751466202854235	26.788346141755188	27.394024046736494	23.066163608654087
64-65	22.695251406418155	26.597968326520345	27.40373434167054	23.303045925390958
66-67	22.666027742585733	26.727695901902432	27.224160021171745	23.382116334340086
68-69	22.59704054902341	26.763830413245927	27.26226713212194	23.376861905608717
70-71	22.638114653041896	26.57474501693923	27.24001509117328	23.54712523884559
72-73	22.56748474948295	26.791698504035956	27.045079584724963	23.59573716175613
74-75	22.5238151719747	26.858382678090177	27.015606781167634	23.602195368767486
76-77	22.52026053458329	26.72014090135627	27.058939814432165	23.700658749628275
78-79	22.470704723043962	26.70206271656645	27.045936147967442	23.781296412422147
80-81	22.470674811624562	26.952781658303703	26.791010872932592	23.785532657139147
82-83	22.567920806184226	26.803859184448477	26.889569894039766	23.73865011532753
84-85	22.540456497735846	26.842185130146934	26.75279822920042	23.864560142916798
86-87	22.336590275252433	26.889026881102936	26.892115517205532	23.8822673264391
88-89	22.332273003719475	26.739592089771808	26.92978342453804	23.998351481970676
90-91	22.47081160891471	26.759238045154643	26.827626669765802	23.942323676164847
92-93	22.425641378905485	26.74249045865632	26.759850555763805	24.07201760667439
94-95	22.31373560102094	26.678923769719944	26.77611975397296	24.231220875286155
96-97	22.272186606409484	26.62245363979973	26.83727322611099	24.26808652767979
98-99	22.20675974655722	26.57629347857391	26.744476835881297	24.472469938987576
100	22.239826067870066	26.607437309518893	26.75417294645091	24.39856367616013
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1212.0
1	875.0
2	729.5
3	980.5
4	881.5
5	858.0
6	1393.0
7	2630.0
8	4513.5
9	5962.5
10	5822.0
11	5322.5
12	5687.0
13	6430.0
14	7216.5
15	8078.5
16	8894.0
17	9770.0
18	10789.0
19	11854.0
20	13160.0
21	14939.5
22	17754.5
23	21753.5
24	26985.0
25	33718.0
26	43125.0
27	55320.5
28	69245.5
29	87073.0
30	108209.5
31	129378.5
32	153723.5
33	180614.0
34	207122.5
35	234203.5
36	267368.5
37	299104.0
38	321612.0
39	341440.5
40	356986.0
41	370597.5
42	386401.0
43	405595.0
44	424982.0
45	440700.0
46	453270.0
47	463121.5
48	474566.0
49	480340.0
50	473538.0
51	460913.5
52	446740.5
53	433833.0
54	417456.0
55	389113.0
56	351520.5
57	314003.5
58	280162.0
59	243489.5
60	204388.0
61	169216.5
62	137980.0
63	107845.0
64	81047.0
65	60650.5
66	44890.5
67	34458.0
68	26556.5
69	19627.5
70	14984.5
71	11436.5
72	8754.5
73	6834.5
74	5143.5
75	3691.5
76	2570.0
77	1585.0
78	953.0
79	576.0
80	355.0
81	236.5
82	150.5
83	93.5
84	54.5
85	40.5
86	33.0
87	26.0
88	19.5
89	18.5
90	12.0
91	6.0
92	4.0
93	2.0
94	3.0
95	3.0
96	0.5
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.031133059321514018
2	0.07901696555766857
3	0.002862810052553013
4	5.112160808130381E-5
5	0.001439925294290057
6	8.520268013550634E-4
7	0.0
8	4.345336686910824E-4
9	5.112160808130381E-5
10-11	8.520268013550634E-6
12-13	7.242227811518039E-5
14-15	0.012452371701804252
16-17	0.0010522530996735033
18-19	1.5336482424391142E-4
20-21	2.513479063997437E-4
22-23	0.0018914994990082406
24-25	8.392463993347374E-4
26-27	0.007561737862026188
28-29	0.008473406539476107
30-31	0.010117818266091377
32-33	0.00906982530042465
34-35	0.004583904191290241
36-37	0.003996005698355247
38-39	0.003131198494979858
40-41	0.003425147741447355
42-43	0.001635891458601722
44-45	0.0052868263024081685
46-47	0.01793090403451731
48-49	0.019034278742272117
50-51	0.013964719274209489
52-53	0.007834386438459809
54-55	0.0012269185939512914
56-57	0.0026668438882413484
58-59	0.002896891124607216
60-61	0.0033697659993592757
62-63	0.0031226782269663076
64-65	0.0037020564518877508
66-67	0.002752046568376855
68-69	0.007046261647206375
70-71	4.941755447859368E-4
72-73	0.005474272198706283
74-75	0.007872727644520787
76-77	0.007242227811518039
78-79	0.004860812901730636
80-81	4.4305393670463297E-4
82-83	0.0012524793979919431
84-85	8.605470693686141E-4
86-87	0.0012609996660054939
88-89	0.0010053916255989748
90-91	0.0010139118936125255
92-93	2.939492464674969E-4
94-95	0.0010565132336802785
96-97	0.0030672964848782285
98-99	0.0035955531017183677
100	0.005521133672780811
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.173672E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.21214306814526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.52932578902653	38.23545900174533
2	16.25131848260098	17.62037605249515
3	5.869246345600096	9.545532677695833
4	2.7347458563632934	5.93025734480737
5	1.426563495576664	3.8668532158997757
6	0.8609130806730477	2.800316585921096
7	0.5325792767208641	2.021058476130457
8	0.3634336296970919	1.5762012743131257
9	0.2485714430424877	1.2128031569587199
>10	1.1007467423708994	10.102253367424407
>50	0.05052645304783035	1.8851214717082103
>100	0.029299855622440186	3.043662137310604
>500	0.0019061306569291342	0.7191357021997768
>1k	7.29313982203332E-4	0.5866115200856905
>5k	2.3526254673711635E-5	0.06773695521939561
>10k+	7.057876402113492E-5	0.7866210600850018
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26873	0.22896516232814618	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23062	0.19649442092850472	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11741	0.10003646674709799	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.520268013550634E-6
2	0.0	0.0	0.0	0.0	1.704053602710127E-5
3	0.0	0.0	0.0	8.520268013550634E-6	1.704053602710127E-5
4	0.0	0.0	0.0	1.704053602710127E-5	1.704053602710127E-5
5	0.0	0.0	0.0	1.704053602710127E-5	3.408107205420254E-5
6	0.0	0.0	0.0	1.704053602710127E-5	4.2601340067753174E-5
7	0.0	0.0	0.0	1.704053602710127E-5	4.2601340067753174E-5
8	0.0	0.0	0.0	1.704053602710127E-5	4.2601340067753174E-5
9	0.0	0.0	0.0	2.55608040406519E-5	5.964187609485444E-5
10-11	0.0	0.0	0.0	4.686147407452849E-5	5.964187609485444E-5
12-13	0.0	0.0	0.0	5.11216080813038E-5	5.964187609485444E-5
14-15	0.0	0.0	0.0	5.538174208807912E-5	1.022432161626076E-4
16-17	0.0	0.0	0.0	8.520268013550635E-5	1.3632428821681015E-4
18-19	0.0	0.0	0.0	1.022432161626076E-4	1.5336482424391142E-4
20-21	0.0	0.0	0.0	1.3632428821681015E-4	1.74665494277788E-4
22-23	0.0	0.0	0.0	3.1098978249459816E-4	2.3856750437941776E-4
24-25	0.0	0.0	0.0	6.603207710501742E-4	2.811688444471709E-4
26-27	0.0	0.0	0.0	0.0015762495825068674	2.8968911246072155E-4
28-29	0.0	0.0	0.0	0.00429421507882952	2.9394924646749686E-4
30-31	0.0	0.0	0.0	0.013419422121342249	2.982093804742722E-4
32-33	0.0	0.0	0.0	0.037169669209114645	3.237701845149241E-4
34-35	0.0	0.0	0.0	0.0767505742660641	3.5785125656912663E-4
36-37	0.0	0.0	0.0	0.13968979408216264	3.6637152458267726E-4
38-39	0.0	0.0	0.0	0.22695437907694826	3.6637152458267726E-4
40-41	0.0	0.0	0.0	0.322027789706153	3.6637152458267726E-4
42-43	0.0	0.0	0.0	0.4181108520949635	3.791519266030032E-4
44-45	0.0	0.0	0.0	0.54174420110559	3.919323286233292E-4
46-47	0.0	0.0	0.0	0.6907764690646109	4.132329986572058E-4
48-49	0.0	0.0	0.0	0.8459901914674628	4.941755447859368E-4
50-51	0.0	0.0	0.0	1.0070786386656578	4.984356787927121E-4
52-53	0.0	0.0	0.0	1.1704164366194303	5.708579569078925E-4
54-55	0.0	0.0	0.0	1.3588847650791704	6.432802350230728E-4
56-57	0.0	0.0	0.0	1.5788227034469595	6.560606370433988E-4
58-59	8.520268013550634E-6	0.0	0.0	1.82101984200015	6.731011730705002E-4
60-61	8.520268013550634E-6	0.0	0.0	2.0746810011655725	6.816214410840508E-4
62-63	8.520268013550634E-6	0.0	0.0	2.3319206728966866	7.284829151585793E-4
64-65	8.520268013550634E-6	0.0	0.0	2.599559331738339	7.327430491653545E-4
66-67	8.520268013550634E-6	0.0	0.0	2.905415652754773	7.455234511856805E-4
68-69	8.520268013550634E-6	0.0	0.0	3.2438747793250586	7.668241212195571E-4
70-71	1.278040202032595E-5	0.0	0.0	3.6089810441077237	8.477666673482882E-4
72-73	2.1300670033876583E-5	0.0	0.0	3.9758850854412477	9.287092134770192E-4
74-75	2.55608040406519E-5	0.0	0.0	4.348084473345193	9.287092134770192E-4
76-77	2.55608040406519E-5	0.0	0.0	4.749022725258846	9.372294814905698E-4
78-79	2.55608040406519E-5	0.0	0.0	5.188975284406546	9.500098835108957E-4
80-81	2.55608040406519E-5	0.0	0.0	5.660146105555896	9.883510895718735E-4
82-83	2.55608040406519E-5	0.0	0.0	6.139449522524181	0.0010820740377209305
84-85	2.55608040406519E-5	0.0	0.0	6.618292845019733	0.0010820740377209305
86-87	2.55608040406519E-5	0.0	8.520268013550634E-6	7.112217041899269	0.0010948544397412566
88	2.55608040406519E-5	0.0	8.520268013550634E-6	7.501652931994629	0.0010991145737480317
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16165	0.0	53.959316	1
GTATCAA	30240	0.0	43.188698	1
TCAACGC	36525	0.0	35.22761	4
ATCAACG	36980	0.0	34.782654	3
CAACGCA	37180	0.0	34.581726	5
TATCAAC	38475	0.0	33.568226	2
AACGCAG	38810	0.0	33.152683	6
ACGCAGA	45205	0.0	28.420982	7
CGCAGAG	47215	0.0	27.158754	8
GTACATG	41275	0.0	24.586432	1
GCAGAGT	53190	0.0	23.763172	9
TACATGG	41865	0.0	23.648712	2
ACATGGG	42845	0.0	22.55162	3
GTGGTAT	8390	0.0	21.364632	1
CATGGGG	29815	0.0	20.884356	4
GAGTACT	28515	0.0	20.548542	12-13
CAGAGTA	51415	0.0	20.232477	10-11
AGAGTAC	48260	0.0	19.913944	10-11
TGGTATC	8475	0.0	19.479324	2
GTACTTT	29705	0.0	19.437944	14-15
>>END_MODULE