FastQCFastQC Report
Mon 6 Jun 2016
ERR522825_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522825_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7596611
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT515800.6789869851174425No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244450.3217882289879No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190820.25119095870513836No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT154760.20372242306470606No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107920.14206334903814347No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG86200.11347165203009606No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA86080.11331368685325602No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG255300.026.1888891
TACATGG260550.025.2458022
ACATGGG265300.023.9973933
CATGGGG174800.022.1365264
GAGTACT210900.021.4304412-13
AGAGTAC324800.019.66391610-11
GTACTTT228100.019.63958514-15
GTATAGC23050.018.7665821
AGTACTT225700.018.31824112-13
ATGGGGG94150.016.9795785
GAGTACA203150.016.3170091
ACTTTTT277100.016.14914916-17
TACTTTT264100.015.56598414-15
AGTACAT195950.015.476652
CATGGGA99700.014.4303664
ATGGGGA79200.014.0699155
GTGTAGC43300.013.790631
TACCTGG52300.013.4850362
TAACCCG4554.816684E-813.4338425
TATACTG36600.013.3604345
Produced by FastQC (version 0.11.5)