##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522825_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7596611
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3631873476212	34.0	31.0	34.0	31.0	34.0
2	32.682449581793776	34.0	31.0	34.0	31.0	35.0
3	32.58327588447006	34.0	31.0	34.0	30.0	35.0
4	35.22940861392008	37.0	35.0	37.0	33.0	37.0
5	35.227648355299486	37.0	35.0	37.0	34.0	37.0
6	35.39107359847701	37.0	35.0	37.0	33.0	37.0
7	35.27046231536668	37.0	35.0	37.0	33.0	37.0
8	35.410706563755866	37.0	35.0	37.0	33.0	37.0
9	36.740438466574105	39.0	35.0	39.0	34.0	39.0
10-11	36.838820165992445	39.0	36.0	39.0	34.5	39.0
12-13	36.90632250354796	39.0	37.0	39.0	33.5	39.0
14-15	38.085042132603604	40.0	37.5	41.0	34.0	41.0
16-17	37.9716485416984	40.0	36.5	41.0	33.5	41.0
18-19	37.98995519186121	40.0	36.5	41.0	34.0	41.0
20-21	38.01791917474779	40.0	36.5	41.0	34.0	41.0
22-23	37.92720780358505	40.0	36.5	41.0	34.0	41.0
24-25	37.93824259265085	40.0	37.0	41.0	34.0	41.0
26-27	37.764110140693006	39.5	36.5	41.0	34.0	41.0
28-29	37.58915074103439	39.0	37.0	41.0	34.0	41.0
30-31	37.50776826666523	39.0	36.5	41.0	33.0	41.0
32-33	37.34748653050683	39.0	36.5	41.0	33.0	41.0
34-35	37.24932848345137	39.0	36.0	41.0	33.0	41.0
36-37	37.136042835417	38.5	36.0	41.0	33.0	41.0
38-39	36.89607523670753	38.0	35.5	41.0	32.0	41.0
40-41	36.855282770172124	38.0	35.5	41.0	32.0	41.0
42-43	36.80358978497122	38.0	36.0	40.0	32.0	41.0
44-45	36.698594741786835	38.0	36.0	40.0	32.0	41.0
46-47	36.406298413858494	38.0	35.5	40.0	30.5	41.0
48-49	36.521593115666974	38.0	36.0	40.0	30.5	41.0
50-51	35.50540905674912	37.5	34.0	39.0	29.5	40.0
52-53	35.64782828816692	37.5	35.5	39.5	30.0	40.0
54-55	36.22190098979664	38.0	36.0	40.0	31.0	41.0
56-57	36.363680001516464	38.0	35.0	40.5	31.0	41.0
58-59	36.35769549079188	38.0	35.0	41.0	31.0	41.0
60-61	36.083448198150464	37.5	35.0	40.0	30.5	41.0
62-63	35.88608644038769	37.5	35.0	40.0	30.5	41.0
64-65	35.645589460879336	37.0	35.0	40.0	30.0	41.0
66-67	35.400827750690404	37.0	35.0	39.0	29.0	41.0
68-69	35.052413043132	37.0	35.0	39.0	29.0	41.0
70-71	34.66020992782177	36.0	34.5	38.5	29.0	41.0
72-73	34.39946424793898	36.0	34.0	38.0	28.0	40.0
74-75	34.132782565804675	36.0	34.0	37.0	28.0	39.0
76-77	33.7841725737964	35.5	34.0	37.0	28.0	39.0
78-79	33.48679128100676	35.0	34.0	37.0	27.0	39.0
80-81	33.25515680610735	35.0	34.0	36.0	27.0	38.5
82-83	33.0282477936543	35.0	33.5	36.0	26.0	38.0
84-85	32.739310792667936	35.0	33.0	36.0	25.5	37.0
86-87	32.65191511056707	35.0	33.0	36.0	25.0	37.0
88-89	32.34821270958852	35.0	33.0	36.0	24.5	37.0
90-91	32.252646147077954	35.0	33.0	35.5	25.0	37.0
92-93	32.02201073610324	35.0	32.0	35.0	24.5	36.0
94-95	31.933545629755166	35.0	32.0	35.0	24.0	36.0
96-97	31.679613711956556	35.0	32.0	35.0	23.0	36.0
98-99	31.630274276252923	35.0	32.0	35.0	22.0	36.5
100	30.393300117644564	34.0	30.0	35.0	18.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.05617858774844109
1101	2	0.02975051077150681
1101	3	0.016377100129510325
1101	4	0.02144497610979812
1101	5	0.052791286089096445
1101	6	0.022700483449149544
1101	7	0.13965416737337222
1101	8	0.09390381680956494
1101	9	-0.016869389032862614
1101	10-11	0.021132246540908284
1101	12-13	0.009512270939502798
1101	14-15	0.04752188475758601
1101	16-17	-0.05121432283526417
1101	18-19	-0.04308367776386035
1101	20-21	0.036657212953222995
1101	22-23	-0.012389575048061374
1101	24-25	-0.06096350381240967
1101	26-27	-0.1359136502561995
1101	28-29	-0.1562087820497382
1101	30-31	-0.09945851647721327
1101	32-33	-0.06265076817410176
1101	34-35	-0.2104228946532416
1101	36-37	-0.11669090570386942
1101	38-39	-0.2078907339674103
1101	40-41	-0.17913299028343488
1101	42-43	-0.15956686629266414
1101	44-45	-0.2194233418655287
1101	46-47	-0.31859514672581923
1101	48-49	-0.2841020718510876
1101	50-51	-0.3906918562015633
1101	52-53	-0.29697142010960675
1101	54-55	-0.24169506308550837
1101	56-57	-0.12896053648146477
1101	58-59	-0.15956293261454135
1101	60-61	-0.08180667728171898
1101	62-63	-0.12077213955436861
1101	64-65	-0.09976321444884206
1101	66-67	-0.4114489964578709
1101	68-69	-0.19524905342987608
1101	70-71	-0.150945958897438
1101	72-73	-0.19536306296727446
1101	74-75	-0.24440792785603804
1101	76-77	-0.1957730950774561
1101	78-79	-0.46796715903442276
1101	80-81	-0.1944869433269787
1101	82-83	-0.3105151490685003
1101	84-85	-0.5676783665059801
1101	86-87	-0.8610940505974263
1101	88-89	-0.3345555800172306
1101	90-91	-0.43803602126683927
1101	92-93	-0.3074217904519685
1101	94-95	-0.5406647296208824
1101	96-97	-0.5125886035563916
1101	98-99	-0.48042111000815524
1101	100	-0.4994929348159616
1102	1	-0.026154351117824604
1102	2	-0.02649805067834876
1102	3	0.05543001174005724
1102	4	0.019958256476108716
1102	5	0.026624759151296473
1102	6	0.026893917790545174
1102	7	0.12415419567761177
1102	8	0.03229137704196461
1102	9	-0.046035680425575265
1102	10-11	0.050960270998764656
1102	12-13	-0.04246651312509897
1102	14-15	-0.01457829912291686
1102	16-17	-0.08265875114852861
1102	18-19	-0.06553334960639745
1102	20-21	-0.10181945337139808
1102	22-23	-0.12309132015781188
1102	24-25	-0.033149636256254666
1102	26-27	-0.12794505332969663
1102	28-29	-0.34991356340580637
1102	30-31	-0.15368530352429843
1102	32-33	-0.11894710703879241
1102	34-35	-0.14686788008962282
1102	36-37	-0.20019094655457792
1102	38-39	-0.2738256761767133
1102	40-41	-0.6587525902033136
1102	42-43	-0.06413529826292574
1102	44-45	-0.16007440146739071
1102	46-47	-0.17930645105260368
1102	48-49	-0.4625849576413046
1102	50-51	-0.3077849348952739
1102	52-53	-0.4022862476949385
1102	54-55	-0.17866528268909576
1102	56-57	-0.009984430192538696
1102	58-59	-0.1442237312662087
1102	60-61	-0.5464650756737441
1102	62-63	-0.13994819031022843
1102	64-65	-0.13695958421668308
1102	66-67	-0.14668077162389181
1102	68-69	-0.3487687347375328
1102	70-71	-0.07960522344414045
1102	72-73	-0.29233329153263554
1102	74-75	-0.13324849789007231
1102	76-77	-0.25855939549467166
1102	78-79	-0.1668438875537035
1102	80-81	-0.23316637334073675
1102	82-83	-0.23945129484297212
1102	84-85	-0.2109259222963331
1102	86-87	-0.2091364984500288
1102	88-89	-0.17455084745798644
1102	90-91	-0.24461418900092724
1102	92-93	-0.22538050105698204
1102	94-95	-0.37612493273333314
1102	96-97	-0.3611252741318829
1102	98-99	-0.4671109084864149
1102	100	-0.6726342533516281
1103	1	-0.08695256914753458
1103	2	-0.07354502254287354
1103	3	-0.0670994179131057
1103	4	-0.0515765275354525
1103	5	0.03599911555924962
1103	6	-0.028106810363198065
1103	7	0.10665540432551524
1103	8	-0.052146298545771685
1103	9	-0.0440599699463462
1103	10-11	-0.03512956596501482
1103	12-13	0.023828183336291886
1103	14-15	-0.0659262166559671
1103	16-17	-0.013279278564674257
1103	18-19	-0.14849882857057395
1103	20-21	-0.14502588264697636
1103	22-23	-0.21614519367559382
1103	24-25	-0.2049150374536879
1103	26-27	-0.26327429069061026
1103	28-29	-0.351276382351422
1103	30-31	-0.24409105669192854
1103	32-33	-0.3872623291773394
1103	34-35	-0.40008627233270744
1103	36-37	-0.46980852341099677
1103	38-39	-0.3220992587626128
1103	40-41	-0.3081466000319253
1103	42-43	-0.22471062448508405
1103	44-45	-0.2703763417033471
1103	46-47	-0.28369776745816466
1103	48-49	-0.23712465733635923
1103	50-51	-0.3401543246143959
1103	52-53	-0.1956897950824299
1103	54-55	-0.15095453074876275
1103	56-57	-0.03985162135303
1103	58-59	0.06394611915249726
1103	60-61	-0.02774203832638733
1103	62-63	-0.03880221493037084
1103	64-65	-0.05157170710516823
1103	66-67	-0.10662375734964513
1103	68-69	-0.08879512035152715
1103	70-71	-0.09466502718569814
1103	72-73	-0.0718186067107851
1103	74-75	-0.07758644679285709
1103	76-77	-0.189180932466968
1103	78-79	-0.19013630336409193
1103	80-81	-0.20397023688120441
1103	82-83	-0.19911495184389594
1103	84-85	-0.16761559968838924
1103	86-87	-0.31558048807477945
1103	88-89	-0.2208445267233472
1103	90-91	-0.2574540151506284
1103	92-93	-0.2638795064349857
1103	94-95	-0.441829720979932
1103	96-97	-0.3189020797849018
1103	98-99	-0.2889123335576862
1103	100	-0.23433461439437053
1104	1	0.02619132937748958
1104	2	0.012369006198355237
1104	3	0.04613007016682502
1104	4	0.04558000860671996
1104	5	0.010105592542430486
1104	6	0.009690175811407187
1104	7	0.13344736217764108
1104	8	0.029807488715384522
1104	9	0.004470329650310134
1104	10-11	-0.06024958934757052
1104	12-13	6.0474921255604386E-5
1104	14-15	0.0401501563090747
1104	16-17	-0.03795423626171868
1104	18-19	-0.0703100875060585
1104	20-21	-0.0588568098066915
1104	22-23	-0.056771630015241215
1104	24-25	0.004501972486707473
1104	26-27	-0.022300347983495783
1104	28-29	-0.14750413130269635
1104	30-31	-0.07804342393294661
1104	32-33	-0.07550090635702134
1104	34-35	-0.14008462185950776
1104	36-37	-0.14556750475733082
1104	38-39	-0.20979656504215427
1104	40-41	-0.044175829759140584
1104	42-43	-0.09614272169233118
1104	44-45	0.013029964177377451
1104	46-47	-0.04762012214690259
1104	48-49	-0.11249738707341805
1104	50-51	-0.1269485077949568
1104	52-53	-0.12465493560642926
1104	54-55	-0.13059743835113125
1104	56-57	-0.6018154570834255
1104	58-59	-0.019839667820470197
1104	60-61	-0.022690849385263334
1104	62-63	-0.06817571982775661
1104	64-65	-0.0797880923184664
1104	66-67	-0.07576227165293403
1104	68-69	-0.20252735125732357
1104	70-71	-0.13954352706395667
1104	72-73	-0.14817119633135434
1104	74-75	-0.07996928555056826
1104	76-77	-0.18053051112363505
1104	78-79	-0.10433737341237048
1104	80-81	-0.05674540793894778
1104	82-83	-0.05388269883810182
1104	84-85	-0.23772168474805255
1104	86-87	-0.2173206770207443
1104	88-89	-0.19750419122761542
1104	90-91	-0.3491347998356673
1104	92-93	-0.516159190617195
1104	94-95	-0.2657386649351281
1104	96-97	-0.27436842916555193
1104	98-99	-0.2853057557832237
1104	100	-0.3664484796723251
1105	1	0.020644927939784452
1105	2	0.008829530005968422
1105	3	0.07839661594223912
1105	4	0.10319936126299467
1105	5	0.057064074311306
1105	6	-0.00412136585356393
1105	7	0.12364700597915146
1105	8	0.025835540327392437
1105	9	0.008218073878438759
1105	10-11	-0.007023799102185535
1105	12-13	0.004279010070568745
1105	14-15	-0.017076627486119378
1105	16-17	-0.04327962076438041
1105	18-19	-0.0018191289786244624
1105	20-21	-0.006890630619757587
1105	22-23	0.00557141491818669
1105	24-25	0.030885414432376024
1105	26-27	-0.04423988917365307
1105	28-29	-0.09869903989650908
1105	30-31	-0.01082860681644604
1105	32-33	-0.0645277864748337
1105	34-35	-0.1302962042489213
1105	36-37	-0.08211584579240849
1105	38-39	-0.05662619289350346
1105	40-41	-0.07076887225262851
1105	42-43	-0.08711453823365645
1105	44-45	-0.09358875654404386
1105	46-47	-0.048653008076698256
1105	48-49	-0.0648875680008274
1105	50-51	-0.13255407096803395
1105	52-53	-0.09034798972157887
1105	54-55	-0.009801356383199789
1105	56-57	-0.013255075037086783
1105	58-59	-0.04134245116808444
1105	60-61	-0.11325397006803684
1105	62-63	-0.08121884319572104
1105	64-65	-0.004054201390047751
1105	66-67	-0.06404263889182005
1105	68-69	0.006501647914824105
1105	70-71	-0.08528422064657093
1105	72-73	-0.02106813664234153
1105	74-75	-0.023442430732472985
1105	76-77	0.014639651208383953
1105	78-79	0.05296960239997617
1105	80-81	-0.030403183832966363
1105	82-83	0.014353686859031711
1105	84-85	-0.031062918437434917
1105	86-87	0.05679979028187887
1105	88-89	0.05753155196820359
1105	90-91	0.02822931517481919
1105	92-93	-0.11187718827795123
1105	94-95	-0.11020853995233537
1105	96-97	-0.11402040870407504
1105	98-99	-0.12468974733610239
1105	100	-0.07621375478942838
1106	1	0.06921908842453917
1106	2	0.029165702435328456
1106	3	0.03957065299543672
1106	4	0.016768889915645957
1106	5	0.01898812418611584
1106	6	0.011737472359584444
1106	7	0.1163196301368501
1106	8	-0.036835714122887
1106	9	-0.04153519517817017
1106	10-11	0.005638534775997073
1106	12-13	-0.01906818585133152
1106	14-15	0.024014807748159228
1106	16-17	-0.07555779331391932
1106	18-19	-0.02643366349196441
1106	20-21	-0.03848277862167038
1106	22-23	-0.047445264981654134
1106	24-25	0.003971249211396355
1106	26-27	-0.09102344238839777
1106	28-29	-0.102591272573342
1106	30-31	-0.15416914384216795
1106	32-33	-0.13778867450895405
1106	34-35	-0.1882929618334046
1106	36-37	-0.18618441787624107
1106	38-39	-0.17388403971948208
1106	40-41	-0.11302840837258543
1106	42-43	-0.10402092498542004
1106	44-45	-0.1524257882027058
1106	46-47	-0.23735609562002224
1106	48-49	-0.33896926752654366
1106	50-51	-0.31894502995535134
1106	52-53	-0.2608463157461145
1106	54-55	-0.22648862542912696
1106	56-57	-0.21564689888614907
1106	58-59	-0.12451086878419204
1106	60-61	-0.15866765621075984
1106	62-63	-0.14514049451256028
1106	64-65	-0.10319384028436218
1106	66-67	-0.10563497263181176
1106	68-69	-0.1251090153522867
1106	70-71	-0.1426223265436377
1106	72-73	-0.09591538215648399
1106	74-75	-0.12888754900815513
1106	76-77	-0.18979547771184713
1106	78-79	-0.19012000550517172
1106	80-81	-0.042793536501669394
1106	82-83	-0.046946086176852475
1106	84-85	-0.06409765874820295
1106	86-87	-0.1013099659465766
1106	88-89	-0.08662783805022656
1106	90-91	-0.015111936773948287
1106	92-93	-0.10899163111970012
1106	94-95	-0.1247807384256916
1106	96-97	-0.05260891785410493
1106	98-99	-0.037946368539163444
1106	100	-0.004422885144435895
1107	1	-0.09897014888358768
1107	2	-0.0917861489366274
1107	3	-0.03731975036266988
1107	4	0.047711317216005966
1107	5	0.027181741562166906
1107	6	0.030670029737287052
1107	7	0.16898452303383493
1107	8	-0.01419978034389402
1107	9	-0.03017390815902843
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2316	70-71	0.3115952800171016
2316	72-73	0.3079773511138697
2316	74-75	0.3689403220322234
2316	76-77	-1.0136150708086404
2316	78-79	0.2962218934873917
2316	80-81	-0.6581324909296171
2316	82-83	0.36403089907782515
2316	84-85	-1.1409802270933724
2316	86-87	0.3776455762930482
2316	88-89	0.5047786491912163
2316	90-91	-0.2810039389620691
2316	92-93	0.4807621995900675
2316	94-95	0.47525121105901036
2316	96-97	0.6399017212128051
2316	98-99	0.5968226286487344
2316	100	0.5892569845800786
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	16210.0
1	1320.0
2	891.0
3	753.0
4	645.0
5	690.0
6	784.0
7	1163.0
8	2043.0
9	2666.0
10	3361.0
11	4340.0
12	5480.0
13	6639.0
14	7833.0
15	9238.0
16	11020.0
17	14033.0
18	17813.0
19	23347.0
20	29790.0
21	38942.0
22	47752.0
23	53024.0
24	54263.0
25	54238.0
26	56370.0
27	61917.0
28	72796.0
29	88586.0
30	112063.0
31	148245.0
32	205716.0
33	301175.0
34	496620.0
35	982459.0
36	1334758.0
37	1374135.0
38	1549050.0
39	403943.0
40	500.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.2443523345583	18.176601451179124	12.96909570881881	23.60995050544377
2	15.70382120044013	20.77389181495319	39.107584450962435	24.414702533644245
3	20.14261608112883	24.835658643383844	29.728868297740956	25.292856977746368
4	12.827519309195981	16.150988693177286	38.23297412073749	32.788517876889244
5	13.469827927831773	37.50183611983232	34.6338169417588	14.394519010577106
6	31.176322890982235	36.43460679271626	17.653305283590452	14.73576503271106
7	27.279835578947537	32.38761212116731	22.105539598343007	18.227012701542147
8	25.68317034555381	33.59925126663632	20.46143109572359	20.256147292086283
9	25.937924339660807	16.40894895538832	20.766968436919527	36.88615826803135
10-11	25.01831425673885	25.422742679990083	28.362884646418845	21.196058416852217
12-13	26.3138411615234	23.363192572122422	27.992255806577347	22.330710459776828
14-15	23.038091997161455	24.997815386857365	26.662054998922873	25.302037617058303
16-17	22.362253645238056	27.899720925640402	26.902462039590997	22.83556338953055
18-19	22.112500839985113	27.607416108095816	28.333202852345206	21.94688019957386
20-21	23.30066170186726	26.029919502335346	28.108611841399096	22.5608069543983
22-23	22.721771022272236	26.025810554283414	28.21904693571533	23.03337148772902
24-25	23.137969805224404	26.09927918856582	28.13304643545983	22.629704570749947
26-27	22.80488478607494	25.806237417468726	28.926008825205347	22.462868971250987
28-29	22.25152357613873	27.09329593487894	28.279396666027797	22.375783822954535
30-31	22.846866271907963	26.088624589832687	28.114197101181787	22.950312037077563
32-33	22.7716182944905	26.320823331021632	28.269849926708023	22.637708447779847
34-35	23.358347333295924	26.170798443446202	28.09791502325259	22.37293920000528
36-37	23.088878612365693	25.949961694094753	28.554763251021026	22.40639644251853
38-39	22.66258691247654	26.584567192256202	28.32802237751379	22.42482351775346
40-41	22.819672429929593	26.6719261473296	27.998222597536742	22.510178825204065
42-43	22.453361652072573	26.754989761722133	27.509751235957562	23.281897350247732
44-45	22.862937360662585	26.20684397344524	27.684577214588284	23.245641451303893
46-47	23.334880629776055	26.645223672790635	27.52194797757001	22.497947719863298
48-49	23.074728510526253	26.572602739545136	27.57876292957539	22.77390582035322
50-51	22.24505133712067	26.897285619558943	28.126519245247543	22.731143798072846
52-53	22.86257833630339	27.27677645162283	27.025194273239983	22.835450938833795
54-55	22.469980140184912	26.567049295317975	27.508333475311797	23.454637089185322
56-57	23.012996337346607	26.503380468868766	27.318268347244	23.16535484654062
58-59	23.598196486274134	26.476619764736053	27.13513879717021	22.790044951819603
60-61	22.95603477933413	26.65417621831221	28.012382088356397	22.377406913997262
62-63	22.47924912539273	26.797504480339988	27.81286111804458	22.9103852762227
64-65	22.354812899850103	27.29704550282354	27.40996610665064	22.938175490675714
66-67	22.741711409515613	26.63290326235351	27.02840227325936	23.59698305487152
68-69	22.38736284034996	26.761971489769763	27.379944718501275	23.470720951378997
70-71	23.015760848891123	26.493109416672656	27.186678434513574	23.30445129992265
72-73	22.746573117299054	26.220687752171408	27.87810165337196	23.154637477157582
74-75	22.22241519822021	27.42507378525333	27.240050159377997	23.112460857148463
76-77	22.4910384207284	26.534871421393298	27.092328201457754	23.881761956420544
78-79	22.58490586221793	26.623812192222562	27.40576349670958	23.385518448849925
80-81	22.966669783812712	26.564074257815452	27.125404898779927	23.34385105959191
82-83	22.673558686241023	26.3135116442844	27.73325702147169	23.279672648002887
84-85	22.47420843560927	26.78209095740181	27.426240309445284	23.31746029754364
86-87	22.391578472989668	26.913682927534772	27.261715933479337	23.433022665996226
88-89	22.146968068039495	26.824085617123792	26.95867541242805	24.070270902408662
90-91	22.580265960190303	26.214621120549857	27.135379549762646	24.069733369497186
92-93	22.900810586458288	26.521899779492802	27.231529386037078	23.34576024801183
94-95	22.62554146618387	26.295805656707323	27.37923003378222	23.69942284332659
96-97	21.842984545133188	26.333800605851494	28.03461400877021	23.78860084024511
98-99	22.335088539139207	26.59396338820146	27.109667914497976	23.96128015816136
100	22.30192428113581	25.54453483548625	27.27323048306511	24.88031040031283
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10445.0
1	8049.5
2	4395.5
3	2589.0
4	1777.5
5	1672.5
6	2309.0
7	3837.5
8	6191.0
9	7635.5
10	7088.0
11	6393.5
12	6527.5
13	6968.5
14	7473.5
15	7889.0
16	8329.5
17	8894.0
18	9548.0
19	10205.0
20	11262.5
21	12847.5
22	15023.5
23	18370.0
24	22788.5
25	28434.0
26	35759.0
27	44081.0
28	53270.5
29	64232.5
30	76175.5
31	88094.0
32	100887.5
33	113618.5
34	126007.5
35	137172.0
36	152836.5
37	169424.5
38	181117.0
39	192741.0
40	204104.5
41	215343.0
42	226416.5
43	238503.0
44	249159.0
45	259944.5
46	275710.0
47	317710.0
48	396246.0
49	393653.0
50	317690.0
51	283838.5
52	275277.5
53	268820.0
54	260338.0
55	245976.0
56	224928.5
57	202019.0
58	180534.0
59	159221.5
60	136434.5
61	114194.0
62	93635.0
63	73236.5
64	54615.5
65	41054.5
66	30432.0
67	23086.0
68	17291.0
69	12436.0
70	9545.0
71	7288.0
72	5526.0
73	4223.0
74	3159.5
75	2372.5
76	1665.5
77	1046.5
78	683.0
79	480.5
80	365.5
81	263.5
82	196.0
83	162.0
84	132.5
85	91.0
86	71.0
87	66.0
88	49.5
89	34.5
90	34.0
91	33.0
92	28.5
93	25.5
94	22.0
95	16.5
96	15.0
97	12.0
98	8.0
99	5.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18907115291279228
2	0.16423112885469587
3	0.3414154022102751
4	0.33708452361191066
5	0.34619384880968634
6	0.2829814505441966
7	0.30125275599869467
8	0.29248568868407243
9	0.3038065263576087
10-11	0.2794469797124007
12-13	0.288332520909653
14-15	0.2751095192316679
16-17	0.2697781945133165
18-19	0.2905506152677819
20-21	0.29426279692352286
22-23	0.2854759839617956
24-25	0.28989900891331677
26-27	0.2813952168934279
28-29	0.3079333666025547
30-31	0.2910574202101437
32-33	0.29107716585724874
34-35	0.2920512844477623
36-37	0.29899517034635575
38-39	0.3042870037704971
40-41	0.3276131948838765
42-43	0.30049583952633613
44-45	0.28563394913863566
46-47	0.2910376745630387
48-49	0.3121326075535525
50-51	0.30209523694184154
52-53	0.328172654885185
54-55	0.32962066900621867
56-57	0.333359178191433
58-59	0.32109713133922485
60-61	0.30395790965208036
62-63	0.3243683268762873
64-65	0.32220288757710513
66-67	0.3096117466064802
68-69	0.3195701346297711
70-71	0.3382955899676843
72-73	0.3421459911531603
74-75	0.3310094461859374
76-77	0.31600933626850186
78-79	0.3262507452336312
80-81	0.3367685932582305
82-83	0.34123769138633003
84-85	0.3341687497227382
86-87	0.33554436313772024
88-89	0.3253358635844326
90-91	0.33588003913850534
92-93	0.31683865344691203
94-95	0.337209579376909
96-97	0.3480367758728201
98-99	0.33711085114138395
100	0.3558297245969288
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7596611.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.39920958389524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.25383402624645	43.36365215706539
2	15.095217609640796	17.63097553799838
3	4.938009985566364	8.651276402233728
4	2.0974584195838473	4.899596553551311
5	1.092890801376033	3.191197948093509
6	0.6295147650983001	2.2057898821879323
7	0.40395779165072965	1.6513571016360913
8	0.27296557478114203	1.2752779048665874
9	0.1885287729356238	0.9908938190935882
>10	0.9702579545028343	9.907175621070921
>50	0.042336584125861394	1.670540087958609
>100	0.013592269880965223	1.2709433217848436
>500	6.890215586142206E-4	0.2765029650758456
>1k	5.167544210320916E-4	0.5655534174230816
>5k	8.612573675784272E-5	0.41434462250363474
>10k+	1.4354289459640453E-4	2.0349226574564483
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	51580	0.6789869851174425	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24445	0.3217882289879	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19082	0.25119095870513836	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	15476	0.20372242306470606	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10792	0.14206334903814347	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	8620	0.11347165203009606	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	8608	0.11331368685325602	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.898258842002046E-5
2	0.0	0.0	0.0	0.0	7.898258842002046E-5
3	0.0	0.0	0.0	0.0	7.898258842002046E-5
4	0.0	0.0	0.0	1.3163764736670076E-5	7.898258842002046E-5
5	0.0	0.0	0.0	1.3163764736670076E-5	7.898258842002046E-5
6	0.0	0.0	0.0	1.3163764736670076E-5	1.0531011789336061E-4
7	0.0	0.0	0.0	1.3163764736670076E-5	1.0531011789336061E-4
8	0.0	0.0	0.0	2.6327529473340152E-5	1.0531011789336061E-4
9	0.0	0.0	0.0	2.6327529473340152E-5	1.3163764736670076E-4
10-11	0.0	0.0	0.0	3.949129421001023E-5	1.5138329447170586E-4
12-13	0.0	0.0	0.0	5.2655058946680304E-5	2.5669341236506647E-4
14-15	0.0	0.0	0.0	6.581882368335038E-5	3.55421647890092E-4
16-17	0.0	0.0	0.0	1.382195297350358E-4	4.6073176578345263E-4
18-19	0.0	0.0	0.0	1.8429270631338106E-4	5.133868247301329E-4
20-21	0.0	0.0	0.0	2.501115299967314E-4	5.528781189401432E-4
22-23	0.0	0.0	0.0	4.475680010467826E-4	6.450244720968337E-4
24-25	0.0	0.0	0.0	6.516063544651687E-4	7.10843295780184E-4
26-27	0.0	0.0	0.0	0.001059683061301941	7.569164723585293E-4
28-29	0.0	0.0	0.0	0.002797300006542391	7.832440018318695E-4
30-31	0.0	0.0	0.0	0.00871441225567559	7.964077665685396E-4
32-33	0.0	0.0	0.0	0.023576302643376106	8.622265902518899E-4
34-35	0.0	0.0	0.0	0.04708678646306886	9.872823552502558E-4
36-37	0.0	0.0	0.0	0.08299095478233649	9.872823552502558E-4
38-39	0.0	0.0	0.0	0.13336210054720454	0.0010004461199869257
40-41	0.0	0.0	0.0	0.18659636514229833	0.0010004461199869257
42-43	0.0	0.0	0.0	0.24225934433130775	0.0010333555318286008
44-45	0.0	0.0	0.0	0.3150681270898299	0.0010991743555119513
46-47	0.0	0.0	0.0	0.40696436871652375	0.0011518294144586315
48-49	0.0	0.0	0.0	0.5005850635237213	0.0013032127089303374
50-51	0.0	0.0	0.0	0.6009850971703039	0.001375613414982023
52-53	0.0	6.581882368335038E-6	0.0	0.7031359115268638	0.0014611778857703784
54-55	0.0	1.3163764736670076E-5	0.0	0.8206422574487492	0.0015796517684004091
56-57	0.0	1.3163764736670076E-5	0.0	0.9687675201481292	0.001605979297873749
58-59	0.0	1.3163764736670076E-5	0.0	1.1282202024034138	0.0016323068273470893
60-61	0.0	1.3163764736670076E-5	0.0	1.3021925171632456	0.0016323068273470893
62-63	0.0	1.3163764736670076E-5	0.0	1.4793043898127731	0.0016783800039254348
64-65	0.0	1.3163764736670076E-5	0.0	1.6707186928486926	0.00171128941576711
66-67	0.0	1.3163764736670076E-5	0.0	1.893758940664462	0.0017310350628721148
68-69	1.3163764736670076E-5	1.3163764736670076E-5	0.0	2.151525199855567	0.00173761694524045
70-71	1.3163764736670076E-5	1.3163764736670076E-5	0.0	2.4302481725074507	0.0017705263570821253
72-73	1.3163764736670076E-5	1.3163764736670076E-5	0.0	2.709359476219067	0.0018692545926071507
74-75	1.3163764736670076E-5	1.3163764736670076E-5	0.0	3.0029772486704926	0.001908745886817161
76-77	1.3163764736670076E-5	1.3163764736670076E-5	0.0	3.323416191772884	0.001908745886817161
78-79	1.3163764736670076E-5	1.3163764736670076E-5	0.0	3.700077574065593	0.001935073416290501
80-81	1.3163764736670076E-5	1.3163764736670076E-5	0.0	4.108904615492356	0.0020272197694471915
82-83	1.3163764736670076E-5	1.3163764736670076E-5	0.0	4.518324026332269	0.0022510037699705826
84-85	1.3163764736670076E-5	1.9745647105005114E-5	0.0	4.931416127533712	0.0022707494170755883
86-87	1.3163764736670076E-5	2.6327529473340152E-5	0.0	5.366880046905127	0.0023563138878639435
88	1.3163764736670076E-5	2.6327529473340152E-5	0.0	5.721827799264698	0.002448460241020634
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	25530	0.0	26.188889	1
TACATGG	26055	0.0	25.245802	2
ACATGGG	26530	0.0	23.997393	3
CATGGGG	17480	0.0	22.136526	4
GAGTACT	21090	0.0	21.43044	12-13
AGAGTAC	32480	0.0	19.663916	10-11
GTACTTT	22810	0.0	19.639585	14-15
GTATAGC	2305	0.0	18.766582	1
AGTACTT	22570	0.0	18.318241	12-13
ATGGGGG	9415	0.0	16.979578	5
GAGTACA	20315	0.0	16.317009	1
ACTTTTT	27710	0.0	16.149149	16-17
TACTTTT	26410	0.0	15.565984	14-15
AGTACAT	19595	0.0	15.47665	2
CATGGGA	9970	0.0	14.430366	4
ATGGGGA	7920	0.0	14.069915	5
GTGTAGC	4330	0.0	13.79063	1
TACCTGG	5230	0.0	13.485036	2
TAACCCG	455	4.816684E-8	13.433842	5
TATACTG	3660	0.0	13.360434	5
>>END_MODULE