FastQCFastQC Report
Mon 6 Jun 2016
ERR522825_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522825_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7596611
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT540870.7119885433122743No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221980.2922092496246023No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183130.2410680236226391No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT154230.2030247435336626No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC120820.15904460554844785No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104450.13749552267451895No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA92490.12175166004946153No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG91330.1202246633400078No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA83400.10978579790382843No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG263800.025.868741
TACATGG266400.025.1773092
ACATGGG270500.023.8190253
CATGGGG177600.021.5924034
GAGTACT216400.019.98501212-13
GTACTTT228200.018.88763414-15
AGAGTAC333500.018.83834310-11
AGTACTT229750.017.53473312-13
ATGGGGG92200.017.2791585
GAGTACA208900.016.2547911
AGTACAT200950.015.7181272
ACTTTTT281800.015.52661516-17
TACTTTT262300.015.3211614-15
CATGGGA101100.015.2467224
GGACCGT10300.015.05672556
GTATAAT32400.014.6608451
GTGTAGC45400.014.6064921
GTATAAG27550.014.3397081
ATACCGT8400.013.4272096
ATGGGGC73200.013.4180375
Produced by FastQC (version 0.11.5)