##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR522825_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7596611 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2523075092301 33.0 31.0 34.0 30.0 34.0 2 32.55777293321982 34.0 31.0 34.0 30.0 34.0 3 32.583209144182845 34.0 31.0 34.0 30.0 34.0 4 35.43974517057672 37.0 35.0 37.0 32.0 37.0 5 35.645128334200606 37.0 35.0 37.0 34.0 37.0 6 35.77766519833647 37.0 35.0 37.0 34.0 37.0 7 35.757655091197904 37.0 35.0 37.0 33.0 37.0 8 35.761766266562816 37.0 35.0 37.0 33.0 37.0 9 37.07452086200017 39.0 36.0 39.0 35.0 39.0 10-11 36.94355812085152 38.5 36.0 39.0 34.0 39.0 12-13 37.01634004952998 38.5 36.0 39.0 34.0 39.0 14-15 38.09520054403207 39.0 37.0 41.0 33.5 41.0 16-17 38.117183965323484 39.0 37.0 41.0 33.5 41.0 18-19 37.98062135865585 39.0 36.5 41.0 32.5 41.0 20-21 37.986812869580916 39.0 37.0 41.0 33.5 41.0 22-23 37.8283494442456 39.0 36.5 41.0 33.5 41.0 24-25 37.82311592629924 39.0 36.5 41.0 33.0 41.0 26-27 37.59307268728121 39.0 36.0 41.0 33.5 41.0 28-29 37.48735291302924 39.0 36.5 41.0 33.0 41.0 30-31 37.21025165037409 38.0 36.0 40.5 32.5 41.0 32-33 37.098539269682234 38.0 36.5 40.0 31.5 41.0 34-35 37.03007275217857 38.0 36.0 40.0 31.0 41.0 36-37 36.930918208132546 38.0 36.0 40.0 32.0 41.0 38-39 36.92430506182296 38.0 36.0 40.0 32.0 41.0 40-41 36.93218199536609 38.0 36.0 40.0 32.0 41.0 42-43 36.89416892611719 38.0 36.0 40.0 32.0 41.0 44-45 36.91602228941301 38.0 35.5 41.0 31.0 41.0 46-47 36.70147155093238 38.0 35.0 40.5 31.0 41.0 48-49 36.537136691611565 38.0 35.5 40.0 31.0 41.0 50-51 36.41089835980807 38.0 35.5 40.0 30.0 41.0 52-53 36.050180073719716 38.0 35.0 40.0 30.0 41.0 54-55 36.09397848593274 37.0 36.0 40.0 29.5 41.0 56-57 35.96349319453109 37.5 35.0 40.0 29.0 41.0 58-59 35.70359466872794 36.5 35.0 40.0 28.5 41.0 60-61 35.471483665545065 37.0 35.0 40.0 28.0 41.0 62-63 35.27325388913556 36.5 34.5 39.5 28.0 41.0 64-65 34.97024699566688 37.0 34.0 39.0 27.5 41.0 66-67 34.70637992388974 36.5 34.0 39.0 28.0 40.5 68-69 34.504318767934805 36.0 34.0 38.0 26.5 40.0 70-71 34.1169197027464 36.0 33.5 37.5 26.0 40.0 72-73 33.83096488684231 35.5 33.0 37.0 26.0 39.5 74-75 33.56665709222178 35.5 33.0 37.0 25.0 39.0 76-77 32.05217589791027 34.0 30.5 36.5 24.5 37.5 78-79 33.01578414374515 35.0 32.5 36.0 26.0 38.0 80-81 33.05094765284151 35.0 33.0 36.0 26.0 37.5 82-83 32.88145608614157 35.0 33.0 36.0 25.5 37.5 84-85 32.80978044025158 35.0 33.0 36.5 25.0 37.0 86-87 32.487559518316786 35.0 33.0 36.0 25.0 36.5 88-89 32.474791390529276 35.0 32.5 35.5 25.0 37.0 90-91 32.36320781727537 35.0 32.0 36.0 24.5 37.0 92-93 32.11983738538146 35.0 32.0 35.0 24.0 36.5 94-95 32.01355420199876 35.0 32.0 35.0 24.5 36.0 96-97 31.76407492762233 35.0 31.5 35.0 24.0 36.5 98-99 31.672135179753184 34.5 32.0 35.0 23.5 36.0 100 30.496055280440185 34.0 29.0 35.0 20.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.14777701004963006 1101 2 -0.21105238817392546 1101 3 -0.21323942342329616 1101 4 -0.12925979875033278 1101 5 -0.16399986984790615 1101 6 -0.13237762895284533 1101 7 -0.17043087910674615 1101 8 -0.15897491543741893 1101 9 -0.2357307593086233 1101 10-11 -0.22667942316305556 1101 12-13 -0.17272058920129751 1101 14-15 -0.2135580710190652 1101 16-17 -0.22803735076850273 1101 18-19 -0.40650542714035964 1101 20-21 -0.2645153744611335 1101 22-23 -0.36485880030262763 1101 24-25 -0.3597913485442703 1101 26-27 -0.41171776764488044 1101 28-29 -0.2630721762884818 1101 30-31 -0.2280831017520697 1101 32-33 -0.2549205891828308 1101 34-35 -0.2743254740988377 1101 36-37 -0.34627151642736465 1101 38-39 -0.3952424514704376 1101 40-41 -0.21946604742023368 1101 42-43 -0.2861193966538096 1101 44-45 -0.4044409782220555 1101 46-47 -0.4059412705292189 1101 48-49 -0.4528646411024724 1101 50-51 -0.4612872099490488 1101 52-53 -0.4601931504929482 1101 54-55 -0.5502616824666688 1101 56-57 -0.5174638410866237 1101 58-59 -0.5540263825563159 1101 60-61 -0.5285338486537796 1101 62-63 -0.4622650258503356 1101 64-65 -0.5067344756995382 1101 66-67 -0.6531438658803239 1101 68-69 -0.7666163631602672 1101 70-71 -0.6749954628441017 1101 72-73 -0.5006544033358935 1101 74-75 -0.6746539110529639 1101 76-77 -0.6444382715642192 1101 78-79 -0.4389455285829058 1101 80-81 -0.3334464437999145 1101 82-83 -0.6908965283420869 1101 84-85 -0.619620934442338 1101 86-87 -0.6046678845932796 1101 88-89 -0.5876105240141634 1101 90-91 -0.6084277268072853 1101 92-93 -0.5192141837350377 1101 94-95 -0.9487868613339927 1101 96-97 -0.6576915269399279 1101 98-99 -0.6782587829030149 1101 100 -0.6138839295597442 1102 1 -0.06163976418850581 1102 2 -0.13665132166403282 1102 3 -0.13065073816066075 1102 4 -0.135734898707625 1102 5 -0.10361500531470114 1102 6 -0.18324420184956125 1102 7 -0.19762517123851353 1102 8 -0.1345485767474912 1102 9 -0.3511686110299337 1102 10-11 -0.21492287446459812 1102 12-13 -0.11058588385147061 1102 14-15 -0.3344412971174009 1102 16-17 -0.31584684348923986 1102 18-19 -0.4244230592506071 1102 20-21 -0.29321283480120997 1102 22-23 -0.23787488936107337 1102 24-25 -0.21746134689680474 1102 26-27 -0.25257030268202385 1102 28-29 -0.32356092262554625 1102 30-31 -0.25381184642224497 1102 32-33 -0.23785494096112103 1102 34-35 -0.19203928577837814 1102 36-37 -0.2716491316387035 1102 38-39 -0.2347866433573813 1102 40-41 -0.2436410832561009 1102 42-43 -0.23642905554062565 1102 44-45 -0.25564323273352585 1102 46-47 -0.30545446366767237 1102 48-49 -0.36328385533781926 1102 50-51 -0.2781363643093471 1102 52-53 -0.2547907253793298 1102 54-55 -0.3198620541708479 1102 56-57 -0.4161625403908502 1102 58-59 -0.41910699588164846 1102 60-61 0.02494965325784193 1102 62-63 -0.31189098040910324 1102 64-65 -0.30727698740471965 1102 66-67 -0.9468268738791537 1102 68-69 -0.3993401918537174 1102 70-71 -0.38018334701447287 1102 72-73 -0.3882502587581129 1102 74-75 -0.4221937195908083 1102 76-77 -0.35259599162523614 1102 78-79 -0.35267366952877666 1102 80-81 -0.4103071976726369 1102 82-83 -0.2668623005199535 1102 84-85 -0.36866910154462573 1102 86-87 -0.3149774553560576 1102 88-89 -0.5049652862741958 1102 90-91 -0.3244576114589037 1102 92-93 -0.5355006437463672 1102 94-95 -1.3223309104301357 1102 96-97 -0.5530523231569866 1102 98-99 -0.7130696425917797 1102 100 -0.6554975164333854 1103 1 -0.09217372625764142 1103 2 -0.14627661189323504 1103 3 -0.11627118426660843 1103 4 -0.1285325432366875 1103 5 -0.15900909656264872 1103 6 -0.09084749481435495 1103 7 -0.18833105180667076 1103 8 -0.17959366243370312 1103 9 -0.10745891892345583 1103 10-11 -0.07686403374865591 1103 12-13 -0.16969440502560218 1103 14-15 -0.18639126118629434 1103 16-17 -0.3079517142174808 1103 18-19 -0.24228056682127175 1103 20-21 -0.17974404425639534 1103 22-23 -0.22430352609246285 1103 24-25 -0.25301746881437026 1103 26-27 -0.2973286198512497 1103 28-29 -0.27531414330066184 1103 30-31 -0.2650305436786269 1103 32-33 -0.2600089867146451 1103 34-35 -0.23431010207128367 1103 36-37 -0.3129639619239484 1103 38-39 -0.2795873982652637 1103 40-41 -0.30722443022904855 1103 42-43 -0.31552990808987147 1103 44-45 -0.28257686291153306 1103 46-47 -0.335806059877811 1103 48-49 -0.33998871099730366 1103 50-51 -0.21218075138270365 1103 52-53 -0.11953909651050765 1103 54-55 -0.23711018273569806 1103 56-57 -0.39141531778895455 1103 58-59 -0.3186205608055914 1103 60-61 -0.4481988998917643 1103 62-63 -0.2224410859177297 1103 64-65 -0.19546875301364963 1103 66-67 -0.09455005918560033 1103 68-69 -0.335757254160292 1103 70-71 -0.3459144455676366 1103 72-73 -0.3702100815520879 1103 74-75 -0.31974916395977004 1103 76-77 -0.3898357530614831 1103 78-79 -0.2482037532651873 1103 80-81 -0.2410786018910187 1103 82-83 -0.009775416444249174 1103 84-85 -0.17770866366718963 1103 86-87 -0.21842604277236433 1103 88-89 -0.27748039842024497 1103 90-91 -0.23251718742276495 1103 92-93 -0.20555075447557414 1103 94-95 -0.8145370732920227 1103 96-97 -0.18836557133131748 1103 98-99 -0.29106963327840063 1103 100 -0.29214798084558424 1104 1 -0.04665132466072208 1104 2 -0.19031524829577506 1104 3 -0.16389078544994362 1104 4 -0.08704856342968981 1104 5 -0.1273510531500932 1104 6 -0.0415826579927554 1104 7 -0.1485043157247503 1104 8 -0.0765480539315675 1104 9 -0.11597622934706209 1104 10-11 -0.16648000235797866 1104 12-13 -0.17942580967284272 1104 14-15 -0.19250918979643217 1104 16-17 -0.2631810316292871 1104 18-19 -0.2960442844636333 1104 20-21 -0.17935046004942734 1104 22-23 -0.9034865367446585 1104 24-25 -0.26151497866930384 1104 26-27 -0.14907569428528689 1104 28-29 -0.21763732230623134 1104 30-31 -0.07437725521295846 1104 32-33 -0.18063393609544676 1104 34-35 -0.15888564813902661 1104 36-37 -0.32644834080821283 1104 38-39 -0.3442952095768348 1104 40-41 -0.19667114868719437 1104 42-43 -0.28145172420314424 1104 44-45 -0.2637247414786046 1104 46-47 -0.27590384658302014 1104 48-49 -0.2931012198724332 1104 50-51 -0.3481415477896803 1104 52-53 -0.2227784312216201 1104 54-55 -0.2314064053748197 1104 56-57 -0.5430730469473204 1104 58-59 -0.4437207452957921 1104 60-61 -0.45484550368544774 1104 62-63 -0.456484446352718 1104 64-65 -0.5301780129603699 1104 66-67 -0.382124448644646 1104 68-69 -0.5307906200346864 1104 70-71 -0.38319968285126293 1104 72-73 -0.4362205786638853 1104 74-75 -0.848528971160988 1104 76-77 -0.5518537146044338 1104 78-79 -0.3301150012400953 1104 80-81 -0.22590849201191077 1104 82-83 -0.21983179313435386 1104 84-85 -0.1982587796729831 1104 86-87 -0.226506846465945 1104 88-89 -0.14897572970163964 1104 90-91 -0.3190208898736202 1104 92-93 -0.24614530551237124 1104 94-95 -0.24800039203595858 1104 96-97 -0.6363740045851323 1104 98-99 -0.4368655637633303 1104 100 -0.40752423380640934 1105 1 -0.035849765708029224 1105 2 -0.13763911928080574 1105 3 -0.10886453730299195 1105 4 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-0.21793298493450663 1105 64-65 -0.2945894911761542 1105 66-67 -0.20598729454542308 1105 68-69 -0.04081057962499557 1105 70-71 -0.07633378474154995 1105 72-73 -0.14872295962887705 1105 74-75 -0.21975203321395043 1105 76-77 -0.24576704794712967 1105 78-79 -0.1225699973554697 1105 80-81 0.013939368602137847 1105 82-83 -0.05297609882588006 1105 84-85 -0.13247727736789727 1105 86-87 -0.21707168861026105 1105 88-89 -0.2768330542486055 1105 90-91 -0.10528561111586754 1105 92-93 -0.06855642452611477 1105 94-95 -0.04234895983577758 1105 96-97 -0.04632420716838581 1105 98-99 -0.17394776855797645 1105 100 -0.2587943140880995 1106 1 -0.04716781363175215 1106 2 -0.1552779562283746 1106 3 -0.17484710953687 1106 4 -0.09302268193533081 1106 5 -0.14723544417578438 1106 6 -0.09369932347277654 1106 7 -0.10223520741550374 1106 8 -0.12575080439369657 1106 9 -0.1669689910811698 1106 10-11 -0.13876006570632882 1106 12-13 -0.0837193427813574 1106 14-15 -0.09687749598588624 1106 16-17 -0.16664438717296548 1106 18-19 -0.22770419509935635 1106 20-21 -0.09626934145499888 1106 22-23 -0.14649754763508582 1106 24-25 -0.1964254415471558 1106 26-27 -0.15613575537570057 1106 28-29 -0.12286866350418535 1106 30-31 -0.12427492567720577 1106 32-33 -0.13476408509033178 1106 34-35 -0.08106182911844684 1106 36-37 -0.17435729707590042 1106 38-39 -0.14111305800808083 1106 40-41 -0.20417955869060478 1106 42-43 -0.1609660392438954 1106 44-45 -0.1557694883398213 1106 46-47 -0.15937703565103334 1106 48-49 -0.0838225963153505 1106 50-51 -0.20084885817001918 1106 52-53 -0.07506737250465534 1106 54-55 -0.1870029863329421 1106 56-57 -0.11748237942414619 1106 58-59 -0.15271784091856944 1106 60-61 -0.190801258581196 1106 62-63 -0.1476583198364878 1106 64-65 -0.22292244691303864 1106 66-67 -0.13124502563547225 1106 68-69 0.2249570526791871 1106 70-71 -0.14314600477399608 1106 72-73 -0.1679049925730638 1106 74-75 -0.22552956054987305 1106 76-77 -0.2590157251665204 1106 78-79 -0.21444089119358978 1106 80-81 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0.10441817843570078 2316 90-91 0.08240645252315915 2316 92-93 0.1491006961140826 2316 94-95 0.23820430033853057 2316 96-97 0.3453610896712682 2316 98-99 0.2494513495520252 2316 100 0.029758388373135602 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 0.0 10 1.0 11 1.0 12 24.0 13 128.0 14 467.0 15 1366.0 16 3154.0 17 6260.0 18 10811.0 19 16806.0 20 24401.0 21 34628.0 22 46046.0 23 54514.0 24 59786.0 25 64967.0 26 73653.0 27 87665.0 28 106568.0 29 131704.0 30 163465.0 31 206294.0 32 265046.0 33 360935.0 34 563842.0 35 1116443.0 36 1275287.0 37 1326808.0 38 1340954.0 39 254470.0 40 115.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.0821477950628 18.904111249017237 13.460270193270102 23.55347076264986 2 15.917026402449515 21.27748343420505 38.441451989244854 24.36403817410058 3 20.146839162884607 25.038349337619103 29.848876031693607 24.965935467802684 4 13.102649819556364 16.671925629446505 37.922808430926914 32.30261612007021 5 13.690542714519069 37.77016824217226 34.46034934797881 14.07893969532985 6 31.31148583300852 36.849540061978 17.264298465346254 14.574675639667234 7 27.30043699749796 32.591757034814606 21.84818993627553 18.259616031411902 8 25.794198658660967 33.979689302618496 20.101327069468557 20.124784969251984 9 25.764647295825622 16.44165612358254 20.572368690390327 37.22132789020151 10-11 25.092111877089295 25.61360802711264 28.195208793612984 21.099071302185074 12-13 26.346850805172657 23.413716438030406 27.915710454959424 22.323722301837513 14-15 22.952456716222393 25.140187008168812 26.531837434744787 25.375518840864007 16-17 22.344700386092203 28.061173949613515 26.827000560718638 22.76712510357564 18-19 22.12270708609405 27.72579772743398 28.24509508253088 21.90640010394109 20-21 23.292822712363183 26.119711303822417 28.07844463963494 22.509021344179462 22-23 22.75419829843363 26.10855877617807 28.16287173222511 22.974371193163197 24-25 23.166120346407478 26.19888067150495 28.092513726349992 22.542485255737578 26-27 22.866231707553077 25.892174566937726 28.845499905673577 22.396093819835617 28-29 22.318126208938548 27.179031711725994 28.221116497409238 22.281725581926224 30-31 22.90231199806587 26.166229506060183 28.04456872623517 22.886889769638774 32-33 22.849654330067903 26.39291676625056 28.210248517299757 22.547180386381783 34-35 23.431801935934853 26.22631586568777 28.08432493059148 22.25755726778589 36-37 23.144422350747423 25.999386554350824 28.562187394481754 22.29400370042 38-39 22.700536823722 26.642152118199025 28.338347632901538 22.318963425177433 40-41 22.90883636343292 26.692104079255728 27.935702628441216 22.463356928870134 42-43 22.52298432958423 26.830604437844897 27.47450792998863 23.171903302582248 44-45 22.942333200113527 26.229387130966654 27.648856020982382 23.179423647937437 46-47 23.402506200202307 26.717495698184873 27.501080678624707 22.378917422988113 48-49 23.15449590399968 26.67683828943217 27.502765895415155 22.665899911152994 50-51 22.348972966141194 26.97483826290276 28.009962267926685 22.66622650302936 52-53 22.962229977156472 27.325479750985515 26.919510786169187 22.79277948568883 54-55 22.557958252431465 26.645522425145245 27.37243306676608 23.424086255657205 56-57 23.12845420189116 26.581907388506043 27.208772197608766 23.08086621199403 58-59 23.720093701848302 26.495948428440446 27.04641058601831 22.737547283692944 60-61 23.00122687111008 26.769682593173755 27.897851132617923 22.331239403098245 62-63 22.547862478733784 26.89540497035762 27.658757468515997 22.8979750823926 64-65 22.41261437762663 27.37557709194039 27.277743870071376 22.93406466036161 66-67 22.79228197942477 26.69640448879112 26.938907362770053 23.57240616901405 68-69 22.457989401830687 26.841196615062408 27.22514494980975 23.475669033297155 70-71 23.097792783939834 26.605178933739026 27.044613347869696 23.25241493445145 72-73 22.808265717107176 26.32099210089247 27.667735006660095 23.203007175340257 74-75 22.26482273329671 27.56006889276717 26.98077566368134 23.194332710254784 76-77 22.552677515922394 26.614526698805456 26.913826877052006 23.918968908220144 78-79 22.58801964937999 26.719175943463537 27.18674124963864 23.506063157517826 80-81 22.958524538803193 26.65907768536729 26.928305518038474 23.454092257791046 82-83 22.677793610454245 26.43189971954683 27.49617897452305 23.39412769547587 84-85 22.44486025991546 26.892654601202082 27.188491461064974 23.473993677817486 86-87 22.35114083782108 26.990295671358673 26.97282735325362 23.685736137566625 88-89 22.076113594607477 26.931974636524558 26.684488691180373 24.30742307768759 90-91 22.49344065410285 26.323369384752016 26.850104786961698 24.333085174183434 92-93 22.797590898305593 26.658768404185686 26.896690397654478 23.646950299854243 94-95 22.493586597779636 26.476359399056843 27.03708452421823 23.992969478945295 96-97 21.69854805482208 26.49783988327728 27.685701683987336 24.117910377913304 98-99 22.13086683665232 26.7510658000334 26.726982415048145 24.391084948266137 100 22.044909236500327 25.72910472841113 26.876195187564562 25.349790847523984 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 958.0 1 733.0 2 801.0 3 1124.5 4 1004.0 5 993.5 6 1591.5 7 3043.5 8 5035.5 9 6300.5 10 5934.0 11 5254.0 12 5432.5 13 5869.5 14 6491.5 15 7051.5 16 7542.0 17 8336.0 18 9192.5 19 10091.5 20 11155.0 21 12570.5 22 14663.5 23 17972.5 24 22505.5 25 28121.5 26 35257.5 27 43682.0 28 53427.0 29 64193.0 30 76152.0 31 88527.5 32 101184.0 33 113935.5 34 126023.5 35 137675.5 36 153773.0 37 169677.5 38 181760.5 39 193249.0 40 204521.5 41 216220.5 42 227059.0 43 238777.0 44 250064.0 45 261559.5 46 275767.0 47 319586.0 48 397685.5 49 393825.0 50 321638.5 51 289353.0 52 278911.0 53 271675.0 54 263440.5 55 248132.0 56 226584.5 57 204009.5 58 182862.0 59 160380.0 60 136967.0 61 114246.0 62 92614.0 63 72018.0 64 54115.0 65 40863.0 66 30021.5 67 22734.0 68 17176.5 69 12235.5 70 9075.0 71 6748.0 72 5041.5 73 3844.0 74 2865.5 75 2119.5 76 1458.0 77 870.5 78 543.5 79 373.0 80 255.0 81 180.5 82 131.0 83 87.0 84 58.5 85 36.0 86 23.0 87 17.0 88 13.0 89 8.5 90 5.0 91 3.0 92 2.0 93 1.0 94 1.5 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05910530366764864 2 0.013032127089303375 3 0.0 4 7.371708252535243E-4 5 2.6327529473340152E-5 6 5.792056484134833E-4 7 0.0 8 3.027665889434118E-4 9 5.2655058946680304E-5 10-11 8.556447078835548E-5 12-13 1.3821952973503578E-4 14-15 0.012551649676414919 16-17 0.0014414322386653731 18-19 0.0 20-21 5.726237660451483E-4 22-23 0.0010662649436702762 24-25 0.0023892232997056186 26-27 0.00853011954936221 28-29 0.007740293665162005 30-31 0.005693328248609807 32-33 0.005522199307033097 34-35 0.0036990178910042914 36-37 0.0023168225936539334 38-39 0.002231258122865578 40-41 0.002481369652862309 42-43 0.0017244531805037798 44-45 0.0018824183573438206 46-47 0.009076415785934017 48-49 0.012762269912201639 50-51 0.012518740264573242 52-53 0.007727129900425335 54-55 0.002849955065489071 56-57 0.0020601291812888667 58-59 0.0023168225936539334 60-61 6.713520015701738E-4 62-63 7.240070605168542E-4 64-65 1.250557649983657E-4 66-67 0.0 68-69 2.1062023578672122E-4 70-71 3.685854126267621E-4 72-73 0.003244868007589174 74-75 0.006029004249394895 76-77 0.005147032012037999 78-79 0.003146139772064148 80-81 9.346272963035754E-4 82-83 0.002046965416552197 84-85 3.356760007850869E-4 86-87 1.3163764736670076E-5 88-89 2.3694776526006134E-4 90-91 9.872823552502556E-5 92-93 4.6073176578345266E-5 94-95 2.501115299967314E-4 96-97 0.0019284915339221663 98-99 0.0011518294144586317 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7596611.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.452665162317594 #Duplication Level Percentage of deduplicated Percentage of total 1 74.41819521349642 43.49941846798493 2 15.012251163710472 17.55012181209962 3 4.896385476458617 8.586203422832114 4 2.114901449377072 4.944865050869527 5 1.0440176741002427 3.051280776386155 6 0.6125601223972011 2.148346303576313 7 0.41087093126449137 1.6811550679093048 8 0.27020198007991025 1.2635220694244962 9 0.1960119855016628 1.0311680660696774 >10 0.9674833231478034 9.872748597777266 >50 0.04253227141754979 1.6842001864654461 >100 0.013272869450792413 1.277800294633248 >500 6.291786366867577E-4 0.25787344754755087 >1k 4.289756007439695E-4 0.48885766039563183 >5k 8.579512013956048E-5 0.44669861852852916 >10k+ 1.7159024027912096E-4 2.2157401575001674 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 54087 0.7119885433122743 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22198 0.2922092496246023 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18313 0.2410680236226391 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 15423 0.2030247435336626 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 12082 0.15904460554844785 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10445 0.13749552267451895 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 9249 0.12175166004946153 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 9133 0.1202246633400078 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 8340 0.10978579790382843 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3163764736670076E-4 2 0.0 0.0 0.0 0.0 1.3163764736670076E-4 3 0.0 0.0 0.0 0.0 1.3163764736670076E-4 4 0.0 0.0 0.0 0.0 1.3163764736670076E-4 5 0.0 0.0 0.0 0.0 1.3163764736670076E-4 6 0.0 0.0 0.0 0.0 1.71128941576711E-4 7 0.0 0.0 0.0 0.0 1.71128941576711E-4 8 0.0 0.0 0.0 0.0 1.9745647105005114E-4 9 0.0 0.0 0.0 0.0 2.237840005233913E-4 10-11 0.0 0.0 0.0 1.3163764736670076E-5 2.237840005233913E-4 12-13 0.0 1.3163764736670076E-5 0.0 2.6327529473340152E-5 4.0807670683677233E-4 14-15 0.0 1.3163764736670076E-5 0.0 3.949129421001023E-5 4.804774128884578E-4 16-17 0.0 1.3163764736670076E-5 0.0 1.0531011789336061E-4 5.792056484134833E-4 18-19 0.0 1.3163764736670076E-5 0.0 1.382195297350358E-4 6.713520015701738E-4 20-21 0.0 1.3163764736670076E-5 0.0 1.9745647105005114E-4 7.10843295780184E-4 22-23 0.0 1.3163764736670076E-5 1.3163764736670076E-5 3.949129421001023E-4 7.700802370951994E-4 24-25 0.0 1.3163764736670076E-5 1.3163764736670076E-5 5.923694131501534E-4 8.55644707883555E-4 26-27 0.0 1.3163764736670076E-5 1.3163764736670076E-5 9.807004728819206E-4 8.7539035498856E-4 28-29 0.0 1.3163764736670076E-5 1.3163764736670076E-5 0.0027117355357540356 8.951360020935652E-4 30-31 0.0 1.3163764736670076E-5 1.3163764736670076E-5 0.008582774608308889 9.017178844619002E-4 32-33 0.0 1.3163764736670076E-5 1.3163764736670076E-5 0.02325379040732769 9.280454139352403E-4 34-35 0.0 1.3163764736670076E-5 1.3163764736670076E-5 0.046718201050442096 9.609548257769156E-4 36-37 0.0 1.3163764736670076E-5 1.3163764736670076E-5 0.08230643901602966 9.872823552502558E-4 38-39 0.0 1.3163764736670076E-5 1.3163764736670076E-5 0.13224318054458759 9.938642376185907E-4 40-41 0.0 1.3163764736670076E-5 1.3163764736670076E-5 0.18517467855073796 0.0010267736494602659 42-43 0.0 1.3163764736670076E-5 1.3163764736670076E-5 0.240633619386329 0.001053101178933606 44-45 0.0 1.3163764736670076E-5 1.3163764736670076E-5 0.31307381673222445 0.0010925924731436162 46-47 0.0 1.3163764736670076E-5 1.3163764736670076E-5 0.404180232474718 0.0011320837673536265 48-49 0.0 1.3163764736670076E-5 1.3163764736670076E-5 0.4968794637503487 0.0011715750615636368 50-51 0.0 1.3163764736670076E-5 1.3163764736670076E-5 0.5964699258656261 0.0011847388263003069 52-53 0.0 1.3163764736670076E-5 1.3163764736670076E-5 0.6979493882206158 0.0012637214147203273 54-55 0.0 1.3163764736670076E-5 1.3163764736670076E-5 0.8146395807288276 0.001382195297350358 56-57 0.0 1.3163764736670076E-5 1.3163764736670076E-5 0.9615801046019075 0.0014545960034020432 58-59 0.0 1.9745647105005114E-5 1.3163764736670076E-5 1.119755901677735 0.0015006691799803887 60-61 0.0 2.6327529473340152E-5 1.3163764736670076E-5 1.2926685333762649 0.0015467423565587337 62-63 0.0 2.6327529473340152E-5 1.3163764736670076E-5 1.4684508657873887 0.0016191430626104192 64-65 0.0 2.6327529473340152E-5 1.3163764736670076E-5 1.6582789351725395 0.0016586343568204295 66-67 0.0 2.6327529473340152E-5 1.3163764736670076E-5 1.8797724406317502 0.0016849618862937697 68-69 0.0 2.6327529473340152E-5 1.3163764736670076E-5 2.135511480053408 0.00171128941576711 70-71 0.0 2.6327529473340152E-5 1.3163764736670076E-5 2.412286215524265 0.0017968538865554655 72-73 0.0 2.6327529473340152E-5 1.3163764736670076E-5 2.689139933583541 0.0019548190633955063 74-75 0.0 2.6327529473340152E-5 1.3163764736670076E-5 2.980486956617892 0.0020206378870788566 76-77 0.0 2.6327529473340152E-5 1.3163764736670076E-5 3.298984244421624 0.0020601291812888667 78-79 0.0 2.6327529473340152E-5 1.3163764736670076E-5 3.674730745065135 0.002073292946025537 80-81 0.0 2.6327529473340152E-5 1.3163764736670076E-5 4.082037371664812 0.002099620475498877 82-83 0.0 2.6327529473340152E-5 1.3163764736670076E-5 4.489804730030273 0.0021588574168138926 84-85 0.0 2.6327529473340152E-5 1.3163764736670076E-5 4.901547545346208 0.0021654392991822275 86-87 0.0 2.6327529473340152E-5 1.3163764736670076E-5 5.3357411614205335 0.002231258122865578 88 0.0 2.6327529473340152E-5 1.3163764736670076E-5 5.68956341189512 0.002303658828917263 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 26380 0.0 25.86874 1 TACATGG 26640 0.0 25.177309 2 ACATGGG 27050 0.0 23.819025 3 CATGGGG 17760 0.0 21.592403 4 GAGTACT 21640 0.0 19.985012 12-13 GTACTTT 22820 0.0 18.887634 14-15 AGAGTAC 33350 0.0 18.838343 10-11 AGTACTT 22975 0.0 17.534733 12-13 ATGGGGG 9220 0.0 17.279158 5 GAGTACA 20890 0.0 16.254791 1 AGTACAT 20095 0.0 15.718127 2 ACTTTTT 28180 0.0 15.526615 16-17 TACTTTT 26230 0.0 15.32116 14-15 CATGGGA 10110 0.0 15.246722 4 GGACCGT 1030 0.0 15.0567255 6 GTATAAT 3240 0.0 14.660845 1 GTGTAGC 4540 0.0 14.606492 1 GTATAAG 2755 0.0 14.339708 1 ATACCGT 840 0.0 13.427209 6 ATGGGGC 7320 0.0 13.418037 5 >>END_MODULE