FastQCFastQC Report
Mon 6 Jun 2016
ERR522824_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522824_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6841463
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT580190.8480496057641472No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254230.3716018050525158No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192660.2816064341793561No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT163280.2386624030561884No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC131450.1921372665466436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120090.17553263095919688No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC110330.16126667644040463No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA91520.13377255712703556No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA85080.12435936582570133No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC77370.1130898464261226No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG54950.031.3158782
GTACATG223850.028.1057281
TACATGG230100.026.7060812
GTACCTG68500.025.8789481
ACATGGG231600.025.7825553
CATGGGG159500.024.877364
TATCACG5900.023.9067442
ATGGGGG106050.022.8316975
ACCTGGG74400.022.4976863
GAGTACT221100.020.47441312-13
AGAGTAC320850.019.59838110-11
AGTACTT230500.018.50816712-13
TGGGGGG111950.018.5073346
ATAACGC5950.018.1748833
GTACTTT245850.018.1458814-15
TATAACG5950.017.3842892
CCTGGGG69800.016.0977444
TAACGCA7500.015.6711654
GAGTACA170200.015.41595651
ACTTTTT296250.015.2638416-17
Produced by FastQC (version 0.11.5)