FastQCFastQC Report
Mon 6 Jun 2016
ERR522824_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522824_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6841463
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT593030.8668175213400994No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT300710.43954049009692814No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230330.3366677565894897No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185500.2711408363971273No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT161470.23601677009727307No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC138490.20242746324872327No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC119290.17436329042486964No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113910.16649947533151901No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA99310.145159010580047No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG93000.13593583711554094No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA89010.13010375120058384No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC81290.11881961504432603No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG58550.030.1860142
GTACATG233350.026.1206131
GTACCTG72350.025.091981
ATAACGC6250.024.8132423
TACATGG241750.024.7323552
ACATGGG237800.024.0902583
TATAACG6850.024.0171972
CATGGGG161300.023.4245514
ACCTGGG74450.023.1028733
TAACGCA7350.023.017854
ATGGGGG99650.021.8820045
GAGTACT219850.020.02549612-13
AGAGTAC326850.019.32359710-11
TATCACG6350.019.2461762
AGTACTT230900.018.45635212-13
GTACTTT240250.018.22406614-15
TGGGGGG101200.017.7390066
CCGTACG2403.4162658E-617.628849
GTATAAC21450.017.5407811
GTATTAG17850.016.8627321
Produced by FastQC (version 0.11.5)