FastQCFastQC Report
Mon 6 Jun 2016
ERR522823_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522823_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6126385
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164020.26772721596830756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123530.20163603821829676No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77590.1266489128580721No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG103350.051.3288542
ACCTGGG108800.047.4609453
GTACCTG146050.036.8688621
CCTGGGG103500.033.5786674
GTACATG248100.027.2007561
TATAACG5350.026.3712312
TACATGG252800.026.1187862
GTATCAA261750.025.746331
ACATGGG255200.025.0807443
CATGGGG151350.024.3919544
CTGGGGG64250.024.0790625
TAACGCA6950.023.6833214
ATGGGGG96150.023.5729835
GGTATCA190150.023.322291
CCGTATC26500.022.8743394
TCAACGC300850.022.2909664
TATCACG6150.022.1761342
CAACGCA304900.022.008145
ATCAACG306300.021.7870453
CGTATCA19700.021.70599694
Produced by FastQC (version 0.11.5)