FastQCFastQC Report
Mon 6 Jun 2016
ERR522823_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522823_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6126385
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157960.25783557513933586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127090.20744696913432636No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT114350.18665167141797323No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77560.12659994433911678No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT61510.10040178669802828RNA PCR Primer, Index 27 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG99500.052.4362372
ACCTGGG110450.046.3425183
GTACCTG143900.036.768131
CCTGGGG100700.034.2597354
TATAACG5900.034.25692
TAACGCA8300.026.0493684
GTACATG250350.025.9996991
GTATCAA267700.025.9133451
CTGGGGG59350.025.1839495
TACATGG256450.024.853562
ATAACGC8900.024.2932323
CATGGGG151100.023.8898934
ACATGGG254250.023.8476833
ATGGGGG89500.022.7395735
TCAACGC303950.022.4688534
GGTATCA195550.022.463061
TGGGGGG87200.022.3669366
CAACGCA306150.022.292045
ATCAACG310850.021.9701083
AACGCAG316200.021.6705826
Produced by FastQC (version 0.11.5)