##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR522822_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2209210 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.18329040697806 29.0 27.0 30.0 21.0 30.0 2 31.55293204358119 34.0 34.0 34.0 26.0 35.0 3 30.607579179887832 34.0 33.0 34.0 14.0 34.0 4 30.978841305262968 34.0 32.0 35.0 20.0 35.0 5 31.244986669442923 35.0 32.0 35.0 13.0 36.0 6 31.594065299360405 35.0 33.0 36.0 22.0 36.0 7 31.755196201357045 35.0 32.0 36.0 15.0 36.0 8 32.17876028082437 35.0 33.0 36.0 22.0 38.0 9 32.25211636738925 36.0 33.0 37.0 21.0 38.0 10-11 32.33417624399672 36.0 33.0 37.0 21.5 37.0 12-13 32.596543787145634 36.0 34.0 36.5 22.5 38.5 14-15 32.56439768061887 36.0 33.5 37.0 21.0 38.0 16-17 32.61790821153263 36.0 33.0 37.0 18.5 38.0 18-19 32.65575499839309 36.0 33.5 37.5 22.0 37.5 20-21 32.873295205073305 36.5 34.0 37.0 21.0 38.0 22-23 32.890926168177764 36.5 33.5 38.0 20.0 38.0 24-25 32.67161360848448 36.5 33.0 37.0 20.0 38.0 26-27 32.840902856677275 37.0 33.5 37.5 21.5 38.0 28-29 32.83146034102688 37.0 33.0 37.5 16.0 38.0 30-31 32.89007767482494 37.0 33.0 38.0 18.0 38.5 32-33 32.82064968925543 37.5 32.5 37.5 13.0 38.0 34-35 32.449513627043146 36.5 32.5 37.5 12.5 37.5 36-37 32.22392257865934 36.5 32.5 37.0 12.0 37.5 38-39 32.30560449210351 36.5 33.0 37.5 12.5 38.0 40-41 32.39950819523722 36.5 32.5 37.5 12.0 39.0 42-43 31.80945428456326 36.0 32.0 37.5 11.5 38.0 44-45 31.709849448445375 36.0 31.5 37.5 11.5 38.0 46-47 31.276802341108358 36.0 30.0 37.0 10.5 38.0 48-49 31.094792708705825 36.0 29.5 38.0 11.0 38.0 50-51 30.97355615808366 36.0 31.0 37.0 10.5 38.5 52-53 31.002201239357056 36.0 30.5 37.0 11.0 38.5 54-55 31.09354520394168 36.5 29.5 38.0 11.0 38.5 56-57 30.85364406280978 36.0 29.5 37.5 11.0 38.0 58-59 31.047238379330167 36.5 30.0 37.5 11.0 38.5 60-61 30.700730804224136 36.5 28.5 37.0 11.0 38.5 62-63 30.546955246445563 36.0 27.5 37.0 11.0 38.0 64-65 30.407249424002245 36.0 27.0 37.0 11.0 38.0 66-67 30.596283286785777 36.0 25.5 38.0 11.0 38.0 68-69 30.482309513355453 36.0 24.5 37.5 10.5 38.0 70-71 29.98142842916699 35.5 24.5 37.5 10.0 38.0 72-73 30.095277271060695 36.0 23.5 37.0 10.0 38.0 74-75 29.68984342819379 35.5 23.0 37.0 9.0 38.0 76-77 29.40619791690242 35.0 23.0 37.0 9.5 38.0 78-79 29.252054580596685 35.5 23.0 37.0 9.0 37.5 80-81 29.064893559236108 35.5 22.5 37.0 9.0 37.5 82-83 29.07181028512455 35.5 21.5 37.0 9.5 38.0 84-85 28.6145117485436 35.5 22.5 37.0 9.5 37.5 86-87 28.611957215475215 35.0 22.0 37.0 9.0 38.0 88-89 28.289226918219633 34.5 22.0 37.0 8.0 37.5 90-91 28.069045722226498 35.0 20.5 37.0 8.5 38.0 92-93 27.97365053571186 34.0 20.5 37.0 8.0 37.5 94-95 27.703203407552927 34.0 19.0 37.0 8.0 38.0 96-97 27.53914295155282 34.0 19.5 37.0 10.0 37.5 98-99 27.581341293946704 33.5 19.5 37.0 7.0 37.5 100 26.676475753776238 33.0 18.0 36.0 10.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.028647892701268773 1101 2 0.009265816132593585 1101 3 -0.08321251223893711 1101 4 -0.05636102575180146 1101 5 0.17173057450364837 1101 6 0.06252716432295458 1101 7 0.12408754907198727 1101 8 -0.24812038933005098 1101 9 -0.09807096748641442 1101 10-11 0.06753492355708346 1101 12-13 -0.11544360907942064 1101 14-15 -0.07780070114416304 1101 16-17 -0.02300508765277698 1101 18-19 -0.171070389265779 1101 20-21 -0.1521899365341497 1101 22-23 -0.1346730917832062 1101 24-25 -0.11988361768050737 1101 26-27 -0.12459486264002351 1101 28-29 0.10539857936652197 1101 30-31 -0.14241171697486976 1101 32-33 -0.026702855997854158 1101 34-35 -0.8035775692330844 1101 36-37 -0.15752360161292955 1101 38-39 -0.2836961232451358 1101 40-41 -0.0948109511979851 1101 42-43 -0.03576612304224014 1101 44-45 -0.16520674777365585 1101 46-47 0.06727205441643491 1101 48-49 0.04542630449989815 1101 50-51 -0.12267745831938726 1101 52-53 -0.03654306618530612 1101 54-55 -0.1584462652028904 1101 56-57 -0.1628159314880726 1101 58-59 -0.6491698665328904 1101 60-61 -0.1001900235782891 1101 62-63 -0.11087223760462805 1101 64-65 -0.023665168736197728 1101 66-67 -0.004544435682525716 1101 68-69 -0.3837494642641346 1101 70-71 -0.17740775332061176 1101 72-73 -0.12742688140263425 1101 74-75 -0.5317197832112122 1101 76-77 -0.9451578294646374 1101 78-79 -0.29105987422632396 1101 80-81 -0.6015092146524559 1101 82-83 -1.4369431078000545 1101 84-85 -1.010816583829719 1101 86-87 -0.6875957502953263 1101 88-89 -1.103295177890029 1101 90-91 -1.1437922164856253 1101 92-93 -1.0597486992426894 1101 94-95 -1.5903674072728187 1101 96-97 -0.9764819129955598 1101 98-99 -1.3061397918119937 1101 100 -1.9531724128125525 1102 1 0.1624419459881814 1102 2 0.23130836456411785 1102 3 0.07665591341316613 1102 4 0.08073916510790724 1102 5 0.2531353858461678 1102 6 -0.06237897884279775 1102 7 0.23909695223340677 1102 8 -0.015007278736291596 1102 9 -1.2975487249897135 1102 10-11 0.16415677664404882 1102 12-13 0.06594547206741197 1102 14-15 0.005423569499093617 1102 16-17 0.045131128850641744 1102 18-19 -0.04973255360088302 1102 20-21 0.011993456573925698 1102 22-23 0.08660846473713235 1102 24-25 -0.024433489392023944 1102 26-27 0.05863115580275746 1102 28-29 0.13540270417338718 1102 30-31 0.003646995106628026 1102 32-33 -0.10574883710764027 1102 34-35 -0.07897029978531833 1102 36-37 -0.04353281601200365 1102 38-39 -0.17105568755366818 1102 40-41 -0.14301386373110603 1102 42-43 0.12014808097490359 1102 44-45 0.015638767722332858 1102 46-47 -0.18429327483404023 1102 48-49 0.034392795687487876 1102 50-51 0.247548776850536 1102 52-53 0.3921271380275897 1102 54-55 0.2893434675236044 1102 56-57 0.1625449891128916 1102 58-59 0.27212567238361274 1102 60-61 0.3717029132142926 1102 62-63 0.2980740490205136 1102 64-65 0.16222764221481256 1102 66-67 0.17283868896354448 1102 68-69 0.2164581743188876 1102 70-71 -0.03047968575767257 1102 72-73 -0.1729623023077167 1102 74-75 -0.12842448199544165 1102 76-77 -0.3478749585990606 1102 78-79 -0.3689103604641524 1102 80-81 -0.6089041544232856 1102 82-83 -0.6201862546781349 1102 84-85 -0.5594172955336845 1102 86-87 -0.6100053366261342 1102 88-89 -0.7349978142708231 1102 90-91 -0.7767756403549733 1102 92-93 -1.094314580797814 1102 94-95 -0.92407686706294 1102 96-97 -0.9579866984706449 1102 98-99 -1.5889491348491127 1102 100 -1.0795977433214432 1103 1 0.1793541851246765 1103 2 0.1814075230569081 1103 3 -0.0135677987307119 1103 4 0.4501474640258145 1103 5 0.3858869984014035 1103 6 0.155101411657796 1103 7 0.09113131270353492 1103 8 0.07917123911732915 1103 9 0.0829579976372159 1103 10-11 0.029672349921256114 1103 12-13 0.06969978968533042 1103 14-15 0.12590402174052429 1103 16-17 0.10459637181439518 1103 18-19 0.026163553537784878 1103 20-21 -0.1077006481263183 1103 22-23 0.001309092933155398 1103 24-25 0.1880068815143332 1103 26-27 0.1479687852125693 1103 28-29 -0.36311076652312124 1103 30-31 0.029587304611389698 1103 32-33 0.005360577826721169 1103 34-35 -0.14332053178837612 1103 36-37 0.06439898139935707 1103 38-39 -0.09261497605502456 1103 40-41 -0.04812430858398642 1103 42-43 -0.06745245990193993 1103 44-45 -0.024443585051219685 1103 46-47 -0.004407963521149583 1103 48-49 0.017614893749573923 1103 50-51 0.2777251181701459 1103 52-53 -0.0049912612157818614 1103 54-55 -0.21628478616744573 1103 56-57 0.10368053195390559 1103 58-59 0.03848071745781567 1103 60-61 -0.08475860426684534 1103 62-63 0.010392973671592642 1103 64-65 -0.022271948328121738 1103 66-67 0.06367852035000254 1103 68-69 -0.03830214572931112 1103 70-71 -0.12620308139497283 1103 72-73 -0.19929442864167157 1103 74-75 -0.002345968009109356 1103 76-77 -1.043603578460747 1103 78-79 -0.9275180164632211 1103 80-81 -0.3487778096095475 1103 82-83 -0.239626611336611 1103 84-85 -0.72142829625761 1103 86-87 -0.538395807369465 1103 88-89 -0.5966898659187265 1103 90-91 -0.6626236235668976 1103 92-93 -0.660197138876601 1103 94-95 -0.729548801104734 1103 96-97 -1.2689562805937555 1103 98-99 -0.8687460414298016 1103 100 -0.9478624798746615 1104 1 -0.027258185267498902 1104 2 0.04159701487430567 1104 3 -0.052735037797862816 1104 4 0.11413442419764053 1104 5 0.26276528970418767 1104 6 0.2390489034533907 1104 7 0.27946110260529977 1104 8 0.060480891424916194 1104 9 0.09690712486275999 1104 10-11 0.08098734463336399 1104 12-13 0.07456650214403737 1104 14-15 0.04900752265091768 1104 16-17 0.09105850070808685 1104 18-19 -0.10949416187559535 1104 20-21 0.09721687168964621 1104 22-23 0.1897971924545203 1104 24-25 0.14475968082528112 1104 26-27 0.12241333868567494 1104 28-29 0.14065192168760632 1104 30-31 0.04098003676067208 1104 32-33 -0.5483285160360509 1104 34-35 -0.004683062997834497 1104 36-37 0.18282242692318817 1104 38-39 -0.011325603078866209 1104 40-41 0.22581369771786797 1104 42-43 0.2539721086171234 1104 44-45 0.09160259949069527 1104 46-47 -0.09483557393992825 1104 48-49 -0.15919340115115688 1104 50-51 -0.06278306443105919 1104 52-53 -0.3090753659269083 1104 54-55 -0.22930684266609802 1104 56-57 -0.1474895636642337 1104 58-59 -0.08465014964714612 1104 60-61 -0.3404652735502012 1104 62-63 -0.20084864474989317 1104 64-65 -0.24181032096628385 1104 66-67 -0.23179755330976093 1104 68-69 -0.2615115134720938 1104 70-71 -0.2676324471752345 1104 72-73 -0.0493794709993054 1104 74-75 -0.30776741830839427 1104 76-77 -0.4240118908060637 1104 78-79 -0.3945055544869689 1104 80-81 -0.37032732497713994 1104 82-83 -0.4186552749722807 1104 84-85 -0.4436758131297971 1104 86-87 -0.6749454862800484 1104 88-89 -0.4136928860127078 1104 90-91 -0.3855706683449682 1104 92-93 -0.4712844817390405 1104 94-95 -0.7259297036440131 1104 96-97 -0.5321801491488003 1104 98-99 -0.8060780010019748 1104 100 -0.6129207556953737 1105 1 -0.08210389393984485 1105 2 -0.008828167178780433 1105 3 0.0851349944484916 1105 4 0.035242759132614765 1105 5 0.06386935359894963 1105 6 0.03199656185559263 1105 7 0.04416180946245518 1105 8 -0.1210472867628809 1105 9 -0.20166421218637254 1105 10-11 -0.05037340877812113 1105 12-13 -0.07058394524742795 1105 14-15 -0.1517856095164234 1105 16-17 -0.029492890583270537 1105 18-19 -0.007525852993971682 1105 20-21 -0.04306309499624206 1105 22-23 0.021800737417891014 1105 24-25 -0.10668243590225046 1105 26-27 0.004326764700579133 1105 28-29 -0.1440744114108199 1105 30-31 -0.08911990558028293 1105 32-33 -0.036594260158040015 1105 34-35 -0.003765216456308451 1105 36-37 -0.01615337763400504 1105 38-39 -0.17573908248177617 1105 40-41 -0.09769752444028512 1105 42-43 -0.21916134003429733 1105 44-45 -0.24044130680268339 1105 46-47 -0.22603253295929804 1105 48-49 -0.30416446485372006 1105 50-51 -0.16526539300218346 1105 52-53 -0.015514599702296294 1105 54-55 -0.017191823553197594 1105 56-57 -0.03263467225542982 1105 58-59 0.1242201602168187 1105 60-61 -0.16174829402983804 1105 62-63 6.261454352589624E-4 1105 64-65 0.1594425886798554 1105 66-67 0.17279616399008546 1105 68-69 -0.07231895362414065 1105 70-71 0.007829999703705681 1105 72-73 -1.1622543585332323E-4 1105 74-75 -0.039239645869415085 1105 76-77 -0.0585526472072857 1105 78-79 -0.22830784803555915 1105 80-81 -0.1847826605741254 1105 82-83 -0.2501063608842564 1105 84-85 -0.19583674592498213 1105 86-87 -0.33914883050453426 1105 88-89 -0.1685864303354272 1105 90-91 -0.9283592304992787 1105 92-93 -0.4091897500671422 1105 94-95 -0.7731358026894348 1105 96-97 -0.6597872988701141 1105 98-99 -0.5937870150323405 1105 100 -0.75338800749455 1106 1 0.06688895905534764 1106 2 -0.07266042356618385 1106 3 0.1432225545413992 1106 4 -0.09111566130167503 1106 5 -0.0806085133053891 1106 6 -0.08646580242896107 1106 7 0.029255476750304155 1106 8 -0.13249405727549401 1106 9 -0.1345053238376508 1106 10-11 -0.1778705982530866 1106 12-13 -0.2649420905372182 1106 14-15 -0.19391056900435188 1106 16-17 -0.21784026810176726 1106 18-19 -0.2187828075664342 1106 20-21 -0.16293895081377485 1106 22-23 -0.16577501171784803 1106 24-25 -0.15649202916104343 1106 26-27 -0.20008217260023287 1106 28-29 -0.22823402222483935 1106 30-31 -0.4610928941558825 1106 32-33 -0.15559467540677474 1106 34-35 -0.36527300827827247 1106 36-37 -0.34546673176354403 1106 38-39 -0.32315934965889426 1106 40-41 -0.34075329099622564 1106 42-43 -0.17956585307918616 1106 44-45 -0.1839518635736006 1106 46-47 0.011364836456785099 1106 48-49 -0.14559409369561394 1106 50-51 -0.3669678284529496 1106 52-53 -0.13111516585851035 1106 54-55 -0.1552415639383291 1106 56-57 -0.1435668550459006 1106 58-59 -0.21616754875795507 1106 60-61 -0.10933369010286853 1106 62-63 -0.12342751786890815 1106 64-65 -0.0153602525668326 1106 66-67 -0.0941870454438245 1106 68-69 0.08751678680150832 1106 70-71 0.1059377905128649 1106 72-73 -0.21096890328384887 1106 74-75 0.03258825884618943 1106 76-77 -0.19152065448595224 1106 78-79 -0.2101331194664482 1106 80-81 -0.04152901786359564 1106 82-83 -0.14251642408309095 1106 84-85 -0.237750283034039 1106 86-87 -0.37087358135528703 1106 88-89 -0.41389167192242127 1106 90-91 -0.3057884778524915 1106 92-93 -0.32919380971441825 1106 94-95 -0.32629713695454754 1106 96-97 -0.2821495831433296 1106 98-99 -0.32980085496914313 1106 100 -0.38951574543545675 1107 1 -0.19637361480981497 1107 2 0.08176267272712678 1107 3 -0.12280618572183144 1107 4 -0.10917023758424094 1107 5 -0.19124341795217958 1107 6 0.0067201363301023775 1107 7 -0.16834180702621993 1107 8 -0.28750891424567726 1107 9 0.023529191338283795 1107 10-11 -0.2092303467757901 1107 12-13 -0.20161593387592802 1107 14-15 -0.23398493779296103 1107 16-17 -0.30389318241817875 1107 18-19 -0.08621498591722343 1107 20-21 -0.4261228330995692 1107 22-23 -0.06336411157011668 1107 24-25 -0.0750321849493858 1107 26-27 -0.1514891780393981 1107 28-29 -0.25627172703278944 1107 30-31 -0.2793119883003854 1107 32-33 -0.21825205856552543 1107 34-35 -0.23739730320171049 1107 36-37 -0.81089531758143 1107 38-39 -0.5372829143244857 1107 40-41 -0.31717876272600876 1107 42-43 -0.546539731671718 1107 44-45 -0.5568916131035486 1107 46-47 -0.20615127957866974 1107 48-49 -0.17073741729217318 1107 50-51 -0.5461206454016434 1107 52-53 -0.3857059276769341 1107 54-55 -0.288024056216063 1107 56-57 -0.2633056409712289 1107 58-59 -0.1058702834477181 1107 60-61 -0.27902001497528417 1107 62-63 6.92185663194067E-4 1107 64-65 -0.07081955957730557 1107 66-67 -0.19924366141212957 1107 68-69 -0.19894700248132935 1107 70-71 -0.16015872943073006 1107 72-73 -0.19537076334294312 1107 74-75 -0.35238353809176104 1107 76-77 -0.24461186956787984 1107 78-79 -0.26268650383389414 1107 80-81 -0.051138623692228435 1107 82-83 -0.3374096553481962 1107 84-85 -0.2434517502647644 1107 86-87 -0.19727743913127327 1107 88-89 -0.24269362934922967 1107 90-91 -0.2357947312099995 1107 92-93 -0.1411198857955327 1107 94-95 -0.08931188480574193 1107 96-97 -0.4104439274221363 1107 98-99 -0.1899529080849085 1107 100 -0.2637607259619159 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-0.030550805231303713 2316 72-73 -0.5393847822781872 2316 74-75 -0.44799181984107506 2316 76-77 0.08270289351807847 2316 78-79 -0.1196637547221826 2316 80-81 -0.24335930646353887 2316 82-83 0.34395920205643904 2316 84-85 0.3881470936572562 2316 86-87 0.5299494262735358 2316 88-89 0.644601245024127 2316 90-91 0.5421496548842875 2316 92-93 0.7675028394795191 2316 94-95 0.8488183899862456 2316 96-97 0.7876699969113687 2316 98-99 1.0393931853101037 2316 100 0.8144391593241629 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 129.0 1 46.0 2 41.0 3 59.0 4 61.0 5 65.0 6 94.0 7 271.0 8 356.0 9 382.0 10 463.0 11 521.0 12 803.0 13 2738.0 14 9889.0 15 23578.0 16 40296.0 17 56060.0 18 68222.0 19 73062.0 20 71261.0 21 69400.0 22 62996.0 23 48796.0 24 36600.0 25 31688.0 26 29299.0 27 30802.0 28 34603.0 29 40728.0 30 49400.0 31 63926.0 32 85130.0 33 116687.0 34 171263.0 35 299103.0 36 558442.0 37 122536.0 38 9414.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.35076049930913 16.32515578510077 14.114917883459524 16.209165832130576 2 15.147203082555643 26.30593314064341 30.195540112987185 28.351323663813755 3 33.20732183584133 22.085840651031212 30.681413515548616 14.025423997578839 4 12.945967722201024 14.901768146179077 41.41979174502587 30.73247238659403 5 10.371565359839945 40.12656650405915 33.28232305952885 16.219545076572057 6 25.846629968798357 33.55893523031597 14.379119903459722 26.215314897425955 7 21.653712897859016 34.13245230493137 22.85490525758893 21.358929539620686 8 24.572570789778116 18.56142995487681 20.610635158039873 36.2553640973052 9 20.08373962087077 23.56893481100609 27.991671331952773 28.35565423617037 10-11 26.952408811214205 21.012894780097742 24.26790188248303 27.766794526205018 12-13 35.25739387134484 21.118612361508195 23.4205203689628 20.20347339818417 14-15 26.320312091366745 24.058061782914088 32.59503953333927 17.0265865923799 16-17 18.813471928500437 27.10334425038615 31.288508879135023 22.79467494197839 18-19 18.315146143301938 32.92155031990538 27.918467099215842 20.844836437576838 20-21 23.974675322910695 23.48932417706349 24.609717168368295 27.92628333165752 22-23 20.107506281805318 24.324943703792272 28.766660144821206 26.80088986958121 24-25 28.23343834293758 24.436471604835393 24.69214946024822 22.63794059197881 26-27 24.259233636948903 18.887835396776403 35.99904076108679 20.853890205187906 28-29 18.730390132205834 32.782037904561086 27.80683782050585 20.680734142727232 30-31 21.638097325585424 23.480961911595845 27.219676242564244 27.66126452025448 32-33 22.229336685483265 24.756377432037002 30.743175624701696 22.271110257778034 34-35 26.192141909138698 22.33731313951872 28.528744126401072 22.94180082494151 36-37 22.302651939380233 20.560742585588258 35.52737119567173 21.60923427935977 38-39 21.19562338270951 25.04089778158117 32.319299270337396 21.44417956537193 40-41 20.153142491256425 27.262999666490895 30.02546488067597 22.55839296157672 42-43 18.733750822083845 27.115495759587354 25.392312904587545 28.758440513741256 44-45 21.942098906158662 21.074513340597374 28.598185831859123 28.38520192138484 46-47 25.541263705584623 26.07339318619662 29.441538128735377 18.943804979483385 48-49 22.371701920260495 24.03239979439777 30.311558963733948 23.28433932160778 50-51 14.231706397197181 25.9672063712611 36.15406169254121 23.647025539000506 52-53 19.803090665718432 28.91210650407793 25.930517775970902 25.354285054232733 54-55 17.230643800637516 22.63893150948631 29.40070092796521 30.729723761910964 56-57 23.063024874229452 20.501940932168253 27.902074577173874 28.532959616428418 58-59 27.77366478752066 20.53274921324911 26.686567502094228 25.007018497136002 60-61 21.32254563581809 22.878444391017588 32.59751420226231 23.201495770902014 62-63 18.634046424781978 23.352588515393542 31.572016546613245 26.44134851321123 64-65 18.661965294364013 28.898950680868886 27.044904519882728 25.394179504884374 66-67 21.961984231908367 23.270761648954487 24.173325384607896 30.593928734529253 68-69 20.371646695862484 22.816664530792142 27.220852897717574 29.590835875627803 70-71 26.100187064518014 23.12205291874535 25.313642124882662 25.464117891853977 72-73 23.790112427318903 18.955769411021038 32.38533015704687 24.868788004613194 74-75 21.151503719301807 27.686714068356167 26.91934836301581 24.242433849326215 76-77 23.017143157318902 22.023473119623517 26.785119109077506 28.17426461398008 78-79 24.133134055887634 22.570706215482243 28.403080439327987 24.893079289302133 80-81 27.24948331322424 20.572531957614707 27.249687045140202 24.928297684020855 82-83 24.285369886828587 19.419463129027186 31.37072211715092 24.924444866993312 84-85 24.404349732470944 22.774795926613486 29.06340949069992 23.757444850215645 86-87 25.273163586217013 22.91608707098507 27.209829972942266 24.600919369855653 88-89 24.86396576632128 22.942101638930126 24.530280661294565 27.66365193345403 90-91 27.826997614130217 18.629638257407255 25.54492527828922 27.998438850173308 92-93 29.62887659974302 20.73622423854779 26.74137511391359 22.893524047795598 94-95 27.84654451665254 19.587336173357386 27.307428981286268 25.258690328703803 96-97 24.749526945861053 20.092835884286362 30.300640111640327 24.85699705821226 98-99 27.82945459139295 22.423455653656728 24.95719928222483 24.789890472725496 100 27.81972080479293 16.202812962576395 25.766351403424803 30.211114829205872 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5976.0 1 3381.5 2 841.5 3 825.0 4 584.0 5 503.0 6 867.0 7 1559.0 8 2089.5 9 1998.0 10 1351.0 11 790.5 12 687.5 13 721.0 14 760.0 15 812.5 16 898.5 17 1058.0 18 1273.0 19 1537.5 20 1889.5 21 2389.0 22 2993.0 23 3707.0 24 4730.0 25 6087.0 26 7602.0 27 9471.5 28 11486.0 29 13465.0 30 15245.5 31 16684.5 32 17898.5 33 18571.5 34 18789.0 35 18382.5 36 17181.0 37 15898.5 38 14901.0 39 13817.0 40 12979.0 41 12665.0 42 12641.5 43 12939.5 44 14295.5 45 21009.0 46 58542.5 47 162960.0 48 317507.5 49 352277.5 50 263888.0 51 172637.0 52 106562.0 53 79209.0 54 53958.5 55 33913.0 56 25133.0 57 21391.0 58 19630.0 59 18747.5 60 18021.0 61 17270.0 62 16448.0 63 15446.0 64 14338.5 65 13296.5 66 12367.0 67 11252.5 68 9932.5 69 8860.0 70 7909.0 71 6789.0 72 5742.5 73 4813.5 74 4014.5 75 3260.5 76 2562.5 77 2013.5 78 1527.5 79 1141.5 80 851.0 81 614.0 82 430.5 83 280.0 84 170.0 85 103.0 86 66.0 87 42.0 88 22.0 89 10.5 90 7.5 91 3.0 92 0.5 93 0.5 94 1.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01801548969993799 2 0.05305063801087266 3 0.09071115919265257 4 0.11280050334735042 5 0.11157834701092245 6 0.07396309087863988 7 0.06730912860253213 8 0.06681121305806148 9 0.06400477998922692 10-11 0.0360762444493733 12-13 0.041259092616817775 14-15 0.0457403325170536 16-17 0.05415963172355728 18-19 0.05845981142580379 20-21 0.05993092553446708 22-23 0.05640025167367521 24-25 0.05241692731791002 26-27 0.0547480773670226 28-29 0.05151162632796339 30-31 0.04727934419996289 32-33 0.0396521833596625 34-35 0.036709955142335944 36-37 0.029716504994998214 38-39 0.02964860742075221 40-41 0.039584285785416506 42-43 0.06364265959324826 44-45 0.06932342330516338 46-47 0.08385350419380683 48-49 0.09213700825181852 50-51 0.08421562458978549 52-53 0.07219775394824395 54-55 0.06438953290995424 56-57 0.050764753011257414 58-59 0.034401437617972035 60-61 0.02638952385694434 62-63 0.02446575925330774 64-65 0.028743306430805582 66-67 0.04026326152787648 68-69 0.04087433969609046 70-71 0.03987850860714916 72-73 0.03444670266746937 74-75 0.054385956971043946 76-77 0.04445027860637966 78-79 0.025529487916495036 80-81 0.01923764603636594 82-83 0.017766531927702663 84-85 0.01740441153172401 86-87 0.018762363016643958 88-89 0.017698634353456668 90-91 0.02632162628269834 92-93 0.03836212944898855 94-95 0.035216208508924006 96-97 0.03118761910366149 98-99 0.032545570588581436 100 0.035804654152389315 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2209210.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.47328179966217 #Duplication Level Percentage of deduplicated Percentage of total 1 91.95364876778511 28.940831001756596 2 3.7077389831508443 2.333894277125988 3 1.2763796316260563 1.205155674885476 4 0.6944187127393933 0.8742254333202233 5 0.4336109506406853 0.6823579820466846 6 0.28873401492911926 0.5452444210207221 7 0.21937676024002375 0.4833154616731835 8 0.17299515714089822 0.4355780264537864 9 0.13789616300169258 0.3906040317519977 >10 0.8964513899639641 5.439259572349304 >50 0.1047507480171683 2.311957366344385 >100 0.08214193987258571 5.58756861955311 >500 0.015714107166700055 3.5256868911309565 >1k 0.01142844157578186 7.295918403268612 >5k 0.0012856996772754592 2.472737731477367 >10k+ 0.003428532472734558 37.47566510584157 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 145234 6.574024198695461 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 93032 4.211098084835756 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 60416 2.7347332304307876 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 43408 1.9648652685801713 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 39933 1.8075692215769439 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 38900 1.7608104254462003 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 38098 1.7245078557493403 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 38092 1.7242362654523562 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 35060 1.586992635376447 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 33446 1.5139348454877535 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 31382 1.420507783325261 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 27475 1.2436572349391863 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 26242 1.1878454289089764 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 25451 1.1520407747565873 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 23984 1.0856369471440017 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 20351 0.921189022320196 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 18530 0.838761367185555 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 17703 0.8013271712512617 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 15388 0.6965385816649391 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 14026 0.6348875842495734 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 12560 0.5685290216864852 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 12177 0.5511925077290072 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12060 0.5458964969378194 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10561 0.4780441877413193 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 8070 0.36528894944346624 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 6988 0.3163121658873534 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 6942 0.3142299736104761 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 6246 0.28272549916033335 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5987 0.27100185134052446 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 5433 0.24592501391900273 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5182 0.2345634864951725 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCCTGTC 5132 0.23230023402030592 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 5017 0.22709475332811277 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCCTGTC 4965 0.2247409707542515 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4957 0.22437885035827285 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 4703 0.21288152778595062 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 4475 0.20256109650055903 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 4257 0.19269331571014073 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4130 0.18694465442397962 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4124 0.18667306412699564 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 3408 0.1542632886869062 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCCTGTCTCTTATACACATCT 3222 0.1458439894804025 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 3138 0.14204172532262663 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 3135 0.14190593017413464 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 3118 0.14113642433268 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCCTGTCTCTTATACACATCT 3094 0.14005006314474405 No Hit GCTTATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 2842 0.12864327067141648 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 2805 0.1269684638400152 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2574 0.11651223740613159 No Hit GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2448 0.11080884116946782 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 2343 0.106056010972248 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 2341 0.10596548087325333 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2335 0.10569389057626935 No Hit GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT 2312 0.10465279443783071 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCCTGTCTCTTATACA 2252 0.10193689146799083 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 9.053009899466324E-5 4.526504949733162E-5 5 4.526504949733162E-5 0.0 0.0 1.3579514849199487E-4 4.526504949733162E-5 6 4.526504949733162E-5 0.0 0.0 1.810601979893265E-4 4.526504949733162E-5 7 4.526504949733162E-5 0.0 0.0 3.62120395978653E-4 4.526504949733162E-5 8 4.526504949733162E-5 0.0 0.0 4.073854454759846E-4 4.526504949733162E-5 9 4.526504949733162E-5 0.0 0.0 5.884456434653111E-4 4.526504949733162E-5 10-11 4.526504949733162E-5 0.0 0.0 8.147708909519692E-4 4.526504949733162E-5 12-13 4.526504949733162E-5 0.0 0.0 0.0011768912869306223 4.526504949733162E-5 14-15 4.526504949733162E-5 0.0 0.0 0.0014484815839146121 9.053009899466324E-5 16-17 4.526504949733162E-5 0.0 0.0 0.0020142947026312572 1.3579514849199487E-4 18-19 4.526504949733162E-5 0.0 0.0 0.00362120395978653 1.3579514849199487E-4 20-21 4.526504949733162E-5 0.0 0.0 0.014801671185627443 1.3579514849199487E-4 22-23 4.526504949733162E-5 4.526504949733162E-5 0.0 0.05427279434730062 2.036927227379923E-4 24-25 4.526504949733162E-5 4.526504949733162E-5 0.0 0.12124243507860274 2.2632524748665813E-4 26-27 4.526504949733162E-5 4.526504949733162E-5 0.0 0.22286247120011227 2.2632524748665813E-4 28-29 4.526504949733162E-5 4.526504949733162E-5 0.0 0.5709733343593411 2.2632524748665813E-4 30-31 4.526504949733162E-5 4.526504949733162E-5 0.0 1.1756464980694457 2.7159029698398975E-4 32-33 4.526504949733162E-5 4.526504949733162E-5 0.0 2.37913100157975 2.7159029698398975E-4 34-35 4.526504949733162E-5 4.526504949733162E-5 0.0 3.8434553528184283 2.7159029698398975E-4 36-37 4.526504949733162E-5 4.526504949733162E-5 0.0 5.325840458806542 2.7159029698398975E-4 38-39 4.526504949733162E-5 4.526504949733162E-5 0.0 7.121346544692447 2.7159029698398975E-4 40-41 4.526504949733162E-5 4.526504949733162E-5 0.0 8.76406045600011 2.7159029698398975E-4 42-43 4.526504949733162E-5 4.526504949733162E-5 0.0 10.332268095835163 2.7159029698398975E-4 44-45 4.526504949733162E-5 4.526504949733162E-5 0.0 12.051638368466556 2.7159029698398975E-4 46-47 4.526504949733162E-5 4.526504949733162E-5 0.0 14.033772253429959 2.7159029698398975E-4 48-49 4.526504949733162E-5 4.526504949733162E-5 0.0 15.29635027905903 2.7159029698398975E-4 50-51 4.526504949733162E-5 4.526504949733162E-5 0.0 16.543017639789788 2.7159029698398975E-4 52-53 4.526504949733162E-5 4.526504949733162E-5 0.0 17.63906554831818 2.7159029698398975E-4 54-55 4.526504949733162E-5 4.526504949733162E-5 0.0 18.86545416687413 2.7159029698398975E-4 56-57 4.526504949733162E-5 4.526504949733162E-5 0.0 20.653491519592976 2.7159029698398975E-4 58-59 4.526504949733162E-5 4.526504949733162E-5 0.0 21.74026009297441 2.7159029698398975E-4 60-61 4.526504949733162E-5 4.526504949733162E-5 0.0 22.848823787688815 3.168553464813214E-4 62-63 4.526504949733162E-5 4.526504949733162E-5 0.0 23.83784248668076 3.168553464813214E-4 64-65 4.526504949733162E-5 4.526504949733162E-5 0.0 24.770506199048526 3.168553464813214E-4 66-67 4.526504949733162E-5 4.526504949733162E-5 0.0 25.933003200239 3.62120395978653E-4 68-69 4.526504949733162E-5 4.526504949733162E-5 0.0 27.237360866554106 3.62120395978653E-4 70-71 4.526504949733162E-5 4.526504949733162E-5 0.0 28.372427247749194 3.62120395978653E-4 72-73 4.526504949733162E-5 4.526504949733162E-5 0.0 29.210645434340783 3.847529207273188E-4 74-75 4.526504949733162E-5 4.526504949733162E-5 0.0 30.32124605628256 4.073854454759846E-4 76-77 4.526504949733162E-5 4.526504949733162E-5 0.0 31.09521050511269 4.073854454759846E-4 78-79 4.526504949733162E-5 4.526504949733162E-5 0.0 32.575966069318895 4.073854454759846E-4 80-81 4.526504949733162E-5 4.526504949733162E-5 0.0 33.811452962823815 4.073854454759846E-4 82-83 4.526504949733162E-5 4.526504949733162E-5 0.0 34.64356036773326 4.073854454759846E-4 84-85 4.526504949733162E-5 4.526504949733162E-5 0.0 35.583240162773116 4.073854454759846E-4 86-87 4.526504949733162E-5 4.526504949733162E-5 0.0 36.37085202402669 4.073854454759846E-4 88 4.526504949733162E-5 4.526504949733162E-5 0.0 37.118834334445346 4.073854454759846E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCCA 915 0.0 49.85966 1 ACCCATG 1090 0.0 42.71862 3 TATAACG 85 3.9357292E-7 38.733578 2 TACCCAT 1280 0.0 36.37758 2 GTATCAT 2960 0.0 30.161116 94 ATCATTA 1610 0.0 30.060545 94 ATAACGC 110 2.9535659E-6 29.930494 3 CATTAAA 915 0.0 29.784641 94 TCATTAA 1165 0.0 27.426378 94 TTATGTA 1735 0.0 24.940025 3 GATATAG 95 0.0012997087 24.753983 1 TATGTAC 1770 0.0 23.914864 4 GTATAAC 160 2.0279676E-6 23.516285 1 GCTTATG 1955 0.0 22.614126 1 CGTATCA 3805 0.0 22.35164 94 GCTTATA 360 0.0 22.209824 1 CTTATGT 1985 0.0 22.03591 2 TATCATT 2070 0.0 21.791462 94 TACCTGG 700 0.0 21.50109 2 ATATGGG 400 0.0 21.165136 3 >>END_MODULE