FastQCFastQC Report
Mon 6 Jun 2016
ERR522822_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522822_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2209210
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT1571997.115620515931035No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1423146.441850254163254No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT931534.216575155824933No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT883323.998352352198297RNA PCR Primer, Index 26 (100% over 22bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT601692.7235527632049465No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT505462.287967191892124RNA PCR Primer, Index 26 (100% over 24bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC438181.9834239388740773No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC400341.8121409915761741No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC380791.723647819808891No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA380061.7203434711955858No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT361411.6359241538830622No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT357501.6182255195296056No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG343851.556438726965748No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC315271.4270712155023741No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA273991.240217091177389No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC257051.1635380973289093No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC249011.1271449975330547No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA246781.11705089149515No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT202650.9172962280634254RNA PCR Primer, Index 26 (100% over 27bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG201310.9112307114307829No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC185660.840390908967459No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT185470.8395308730270097No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG150620.6817821755288089No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC142390.644529039792505No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG134770.6100370720755384RNA PCR Primer, Index 26 (96% over 27bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG126270.5715617800028064No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT122070.5525504592139271No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100240.4537368561612522No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94510.42779998279928116No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC92390.41820379230584687RNA PCR Primer, Index 26 (95% over 22bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG74410.33681723330964464No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT73500.33269811380538744No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT72360.32753789816269163RNA PCR Primer, Index 26 (100% over 21bp)
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG67340.30481484331503117No Hit
CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC64900.29377017123768223No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA64420.2915974488618103No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT56270.25470643352148503No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53930.24411441193910943No Hit
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGC53260.24108165362278824RNA PCR Primer, Index 26 (96% over 28bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCCTGTC50270.22754740382308608No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49520.22415252511078623No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCCTGTC49450.22383566976430488No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA48690.22039552600250767No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA48640.22016920075502103No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA48610.22003340560652904No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA48570.21985234540853968No Hit
CTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC47750.21614061134975854RNA PCR Primer, Index 26 (95% over 22bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT42740.19346282155159536No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC40380.1827802698702251No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40220.1820560290782678No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG36210.1639047442298378RNA PCR Primer, Index 26 (100% over 29bp)
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT35810.16209414224994453No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCCTGTCTCTTATACACATCT32020.14493868849045588No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT31230.14136274958016667No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC31150.14100062918418801No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCCTGTCTCTTATACACATCT30690.13891843690731076No Hit
GCTTATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT29070.13158549888874302No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA28920.13090652314628307No Hit
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC28470.12886959591890315No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG25940.11741753839607824No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG25650.11610485196065562TruSeq Adapter, Index 1 (96% over 28bp)
GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24240.10972247998153185No Hit
AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG23360.10573915562576669No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT23220.10510544493280403No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG22850.10343063810140277No Hit
GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT22800.10320431285391611No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGCTGTCTCTTATACA22190.10044314483457888No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCA7050.069.383511
CTTTACA10100.065.661341
CTATACA8200.060.2264751
ACCCATG9450.051.72483
TACCCAT10000.048.409942
GAGATGT4000.045.858161
GATGTGT4150.044.1686253
ATGTGTA4400.041.659044
CTTAATA2100.040.314861
TGTATAA4550.040.283847
TGTGTAT4650.039.4193085
GTGTATA4650.039.4175226
CTTAACA3700.039.406871
TCTATAC1750.037.627211
GCTTATA4050.037.162671
TAAGGCG5200.036.60406518-19
TATAACG651.4139217E-436.15382
ACTAAGG5300.035.91342516-17
GCGAATC5250.035.3602622-23
TTACACA18800.035.2499583
Produced by FastQC (version 0.11.5)