FastQCFastQC Report
Mon 6 Jun 2016
ERR522821_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522821_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13498548
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT344970.255560820319341No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280600.20787420987798097No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154530.11447897951690804No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA254600.037.314771
GTATCAA391800.033.8150671
TCAACGC463400.028.0318814
GTACATG450550.027.7250841
CAACGCA472000.027.5688025
ATCAACG473900.027.430743
TATCAAC486950.026.869762
AACGCAG490050.026.5905936
TACATGG463100.026.5169222
ACATGGG466700.025.445433
CATGGGG321550.023.6800694
TATAACG11800.023.515872
ACGCAGA557350.023.209037
TACCTGG129850.023.1808342
CGCAGAG579800.022.3257588
ATGGGGG186400.020.4861765
GAGTACT318200.019.97651712-13
GCAGAGT644650.019.8163369
ACCTGGG155000.018.9338553
AGAGTAC526150.018.67200910-11
Produced by FastQC (version 0.11.5)