FastQCFastQC Report
Mon 6 Jun 2016
ERR522821_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522821_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13498548
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325340.24101851547292347No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285620.21159312838684574No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT170590.12637655546359505No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154920.11476789948074416No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA259200.039.1048431
GTATCAA391350.032.774651
TCAACGC467150.026.9646074
CAACGCA474300.026.5190735
ATCAACG479250.026.2937163
GTACATG447400.026.2191581
TATCAAC492250.025.7158032
AACGCAG492300.025.671296
TACATGG455050.025.3905562
ACATGGG459400.024.2683933
TACCTGG125200.022.7519052
ACGCAGA564750.022.3115087
CATGGGG324050.022.0468864
CGCAGAG588800.021.3676958
GAGTACT339250.019.77554912-13
GCAGAGT656050.018.9695999
ATGGGGG184700.018.6281435
GTACTTT357200.018.43970314-15
AGAGTAC543550.018.35653910-11
ACCTGGG149450.018.2095813
Produced by FastQC (version 0.11.5)