##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR522820_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6807 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.78639635669164 30.0 22.0 31.0 10.0 34.0 2 25.909064198619067 30.0 22.0 31.0 10.0 34.0 3 25.444101660055825 30.0 18.0 31.0 8.0 34.0 4 27.8282650213016 33.0 22.0 35.0 10.0 37.0 5 27.688555898339946 33.0 22.0 35.0 7.0 37.0 6 27.966945791097398 33.0 23.0 35.0 8.0 37.0 7 27.749522550315852 33.0 20.0 35.0 8.0 37.0 8 27.7589246364037 33.0 21.0 36.0 8.0 37.0 9 28.65535478184222 33.0 22.0 38.0 8.0 39.0 10-11 28.598060819744383 33.0 21.5 37.5 8.0 39.0 12-13 28.534302923461144 33.5 20.5 37.0 8.0 39.0 14-15 29.03973850448068 33.5 20.0 39.0 8.0 41.0 16-17 28.849786983987073 33.5 20.0 38.5 8.0 41.0 18-19 28.90149845747025 34.0 20.0 38.5 8.0 41.0 20-21 28.83252534156016 34.0 18.5 39.0 8.0 41.0 22-23 28.736007051564567 33.5 18.5 39.0 8.0 41.0 24-25 28.653518436903187 33.0 18.0 38.5 8.0 41.0 26-27 28.444101660055825 33.5 18.0 38.5 8.0 41.0 28-29 28.109593065961512 33.0 17.0 38.0 8.0 40.0 30-31 28.119362421037167 33.0 17.5 38.0 8.0 40.0 32-33 27.87836051123843 33.0 16.5 38.0 8.0 40.0 34-35 27.738063757896285 32.0 16.5 38.0 8.0 40.0 36-37 27.525708829146467 33.0 15.5 38.0 8.0 40.0 38-39 27.26773909211106 32.0 15.5 38.0 8.0 40.0 40-41 27.172175701483766 32.0 15.0 38.0 8.0 40.0 42-43 26.900029381519026 31.5 14.5 38.0 8.0 40.0 44-45 26.878287057440872 32.0 14.5 38.0 8.0 40.0 46-47 26.613486117232263 30.5 13.5 38.0 7.5 40.0 48-49 26.565300426032024 31.0 13.0 37.5 7.0 40.0 50-51 25.8269428529455 30.0 13.0 36.5 7.5 39.0 52-53 26.222271191420596 30.5 13.5 37.0 7.0 39.0 54-55 26.793521375055093 31.0 14.0 37.0 7.0 40.0 56-57 26.875201997943293 31.5 14.5 37.5 7.0 40.0 58-59 26.683046863522847 31.0 13.5 38.0 7.0 40.0 60-61 26.489349199353605 31.0 13.0 37.0 7.0 40.0 62-63 26.34971353018951 31.0 13.0 37.0 7.0 40.0 64-65 26.01579256647569 30.0 13.0 36.5 7.0 40.0 66-67 25.798148964301454 30.0 13.0 37.0 7.0 39.0 68-69 25.577787571617453 30.0 13.0 36.0 7.0 39.0 70-71 25.15586895842515 29.0 11.5 35.5 7.0 38.5 72-73 25.02585573674159 29.0 11.5 35.0 7.0 38.0 74-75 24.695754370500957 29.0 11.0 35.0 7.0 37.0 76-77 24.570809460849127 29.0 11.0 35.0 7.0 37.0 78-79 24.364404289701778 28.0 11.0 35.0 7.0 37.0 80-81 24.095343029234613 28.0 10.5 35.0 7.0 36.0 82-83 23.88497135301895 28.5 10.0 35.0 7.0 36.0 84-85 23.602321140002935 28.0 10.0 34.0 7.0 35.5 86-87 23.54855296018804 27.0 10.0 34.0 7.0 36.0 88-89 23.134200088144556 27.0 10.0 34.0 7.0 35.0 90-91 23.055971793741737 26.5 10.0 34.0 7.0 35.0 92-93 22.9031144410166 27.0 10.0 34.0 7.0 35.0 94-95 22.670339356544734 26.0 10.0 34.0 7.0 35.0 96-97 22.43793154106067 25.5 10.0 34.0 7.0 35.0 98-99 22.449243425885115 25.5 10.0 34.0 7.0 35.0 100 21.518289995592774 24.0 9.0 33.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.6435981842231797 1101 2 0.12775184884560176 1101 3 -1.423261634199136 1101 4 -1.8183606902356857 1101 5 -2.9787743506493634 1101 6 0.1979117815055318 1101 7 -1.1323626893939398 1101 8 -1.0525586970899496 1101 9 -2.2609747023809597 1101 10-11 -1.370181727994229 1101 12-13 -2.3011275327681666 1101 14-15 -2.011909647065881 1101 16-17 -3.0187469937469764 1101 18-19 -3.3831176346801435 1101 20-21 -2.7641333348364583 1101 22-23 -2.050697450697445 1101 24-25 -3.694155468374216 1101 26-27 -0.43685647396585026 1101 28-29 -2.3554546581890285 1101 30-31 -2.6018403905122582 1101 32-33 -4.144645299723422 1101 34-35 -2.7215187590187604 1101 36-37 0.9396122685185126 1101 38-39 3.085801091269854 1101 40-41 1.0815960948773409 1101 42-43 -2.9132649034992717 1101 44-45 -2.5682180811087 1101 46-47 0.31425057870369955 1101 48-49 0.12341532888407514 1101 50-51 -0.4251893939393945 1101 52-53 -0.30403551887926383 1101 54-55 0.8206033549783562 1101 56-57 0.713569474506965 1101 58-59 -1.4165054563492134 1101 60-61 -0.8275167974386584 1101 62-63 -1.7913271479677668 1101 64-65 -0.6142458814333835 1101 66-67 1.1192813552188632 1101 68-69 -1.1080821233164926 1101 70-71 -0.4387827305796037 1101 72-73 -0.7512932524651319 1101 74-75 1.9516667794011475 1101 76-77 1.1163912187349574 1101 78-79 -2.2457465277777686 1101 80-81 -1.3509866146584883 1101 82-83 -1.9028510551948052 1101 84-85 -5.035851446007694 1101 86-87 -2.742127750721508 1101 88-89 0.17721805104617872 1101 90-91 -1.6364773103054269 1101 92-93 -0.32949096620972185 1101 94-95 -1.294802564333807 1101 96-97 0.7566141323953772 1101 98-99 -3.5574276996151966 1101 100 0.9305781024530937 1102 1 1.1064018157768203 1102 2 1.044418515512266 1102 3 -2.839928300865804 1102 4 -0.73502735690235 1102 5 3.604558982683969 1102 6 -0.8854215518278039 1102 7 0.3676373106060602 1102 8 1.030774636243386 1102 9 -1.7609747023809597 1102 10-11 1.2964849386724353 1102 12-13 -0.3427941994348309 1102 14-15 -0.5952429803992167 1102 16-17 -0.18541366041364782 1102 18-19 -1.7164509680134756 1102 20-21 0.3608666651635417 1102 22-23 2.574302549302555 1102 24-25 1.4725111982924517 1102 26-27 0.9798101927008176 1102 28-29 -2.5221213248556964 1102 30-31 1.2731596094877382 1102 32-33 1.6470213669432425 1102 34-35 -2.2215187590187604 1102 36-37 -0.6020543981481552 1102 38-39 -2.4558655753968175 1102 40-41 -2.835070571789327 1102 42-43 1.8367350965007248 1102 44-45 3.0984485855579678 1102 46-47 -0.2690827546296326 1102 48-49 3.040081995550743 1102 50-51 -1.6751893939393945 1102 52-53 2.445964481120736 1102 54-55 -0.9293966450216402 1102 56-57 -4.828097192159699 1102 58-59 1.0001612103174544 1102 60-61 -0.07751679743865836 1102 62-63 0.7086728520322332 1102 64-65 0.8024207852332808 1102 66-67 0.4109480218855275 1102 68-69 -2.5664154566498247 1102 70-71 -1.7304493972462716 1102 72-73 -1.4596265857984676 1102 74-75 -1.9649998872655239 1102 76-77 1.199724552068293 1102 78-79 3.587586805555567 1102 80-81 -1.6843199479918205 1102 82-83 -2.0695177218614695 1102 84-85 0.6724818873256382 1102 86-87 1.9245389159451562 1102 88-89 0.13555138437951442 1102 90-91 -0.5114773103054304 1102 92-93 1.6288423671236139 1102 94-95 1.4968641023328573 1102 96-97 -1.0350525342712942 1102 98-99 -0.30742769961519656 1102 100 1.1805781024530937 1103 1 -3.1793124699374644 1103 2 1.0682280393217916 1103 3 0.23150027056276912 1103 4 1.3840202621452669 1103 5 -3.8716314935065057 1103 6 -5.147326313732563 1103 7 -3.894267451298699 1103 8 -5.778749173280424 1103 9 -2.5466889880952444 1103 10-11 -3.9535150613275647 1103 12-13 -1.8785084851491156 1103 14-15 -4.261909647065881 1103 16-17 -1.0306517556517427 1103 18-19 -2.0021652537277603 1103 20-21 -1.3891333348364583 1103 22-23 -3.3661736411736385 1103 24-25 -3.729869754088501 1103 26-27 -0.8535231406325146 1103 28-29 -0.7245022772366454 1103 30-31 -4.881602295274163 1103 32-33 -0.6625024425805641 1103 34-35 -0.3048520923520961 1103 36-37 -2.0068163029100603 1103 38-39 1.5917534722222335 1103 40-41 -3.9660229527417066 1103 42-43 -1.4965982368326074 1103 44-45 1.907972395081778 1103 46-47 -1.4476541832010632 1103 48-49 -1.3289656234968774 1103 50-51 -1.3537608225108215 1103 52-53 -4.31594028078403 1103 54-55 -4.73892045454545 1103 56-57 -2.9828590969216044 1103 58-59 2.131113591269834 1103 60-61 -2.2322787022005635 1103 62-63 -2.755612862253482 1103 64-65 -2.62615064333815 1103 66-67 -0.5533376924001878 1103 68-69 -1.2926059328403028 1103 70-71 -0.8733065401034175 1103 72-73 -3.1977218238937013 1103 74-75 1.8326191603535271 1103 76-77 2.544962647306388 1103 78-79 -0.7100322420634839 1103 80-81 0.8156800520081795 1103 82-83 -3.1171367694805205 1103 84-85 -2.458470493626745 1103 86-87 -1.242127750721508 1103 88-89 1.3736466224747481 1103 90-91 -0.8090963579244779 1103 92-93 -1.2640147757335285 1103 94-95 0.008768864237623575 1103 96-97 -1.8088620580808161 1103 98-99 0.5259056337181391 1103 100 1.7520066738816666 1104 1 -1.6435981842231797 1104 2 0.2110851821789339 1104 3 0.16007169913419617 1104 4 1.3483059764309822 1104 5 -6.228774350649363 1104 6 -0.21875488516113606 1104 7 0.28430397727272805 1104 8 3.114107969576718 1104 9 0.9890252976190403 1104 10-11 2.9214849386724353 1104 12-13 -2.092794199434831 1104 14-15 -0.38690964706588105 1104 16-17 -0.6020803270803121 1104 18-19 -0.46645096801347563 1104 20-21 6.360866665163542 1104 22-23 -1.2590307840307808 1104 24-25 -0.6941554683742162 1104 26-27 2.1464768593674854 1104 28-29 1.4362120084776393 1104 30-31 -0.5601737238455939 1104 32-33 -0.39464529972342177 1104 34-35 -2.179852092352096 1104 36-37 -1.9353877314814874 1104 38-39 -2.497532242063482 1104 40-41 2.4982627615440087 1104 42-43 0.003401763167392602 1104 44-45 -7.484884747775364 1104 46-47 -0.6440827546296326 1104 48-49 2.4567486622174073 1104 50-51 -0.17518939393939448 1104 52-53 1.1126311477874005 1104 54-55 0.6539366883116919 1104 56-57 1.213569474506965 1104 58-59 1.6668278769841187 1104 60-61 -1.285850130771994 1104 62-63 -2.291327147967767 1104 64-65 3.9274207852332808 1104 66-67 4.4109480218855275 1104 68-69 1.1002512100168396 1104 70-71 2.1445506027537284 1104 72-73 0.6237067475348681 1104 74-75 1.8683334460678118 1104 76-77 0.9913912187349574 1104 78-79 1.2542534722222314 1104 80-81 -0.6843199479918205 1104 82-83 2.0971489448051948 1104 84-85 3.5058152206589703 1104 86-87 -0.11712775072150805 1104 88-89 -0.5727819489538213 1104 90-91 1.6551893563612374 1104 92-93 1.1288423671236139 1104 94-95 6.205197435666193 1104 96-97 4.6732807990620415 1104 98-99 -1.7240943662818609 1104 100 1.1805781024530937 1105 1 -0.32216961279460676 1105 2 -5.503200532106781 1105 3 -2.768499729437231 1105 4 -1.330265452140445 1105 5 -3.585917207792221 1105 6 -1.290183456589709 1105 7 -1.894267451298699 1105 8 -3.778749173280424 1105 9 -4.689546130952387 1105 10-11 -2.8106579184704223 1105 12-13 -6.807079913720546 1105 14-15 -4.761909647065881 1105 16-17 -3.5306517556517427 1105 18-19 -5.716450968013476 1105 20-21 -4.103419049122174 1105 22-23 -6.151887926887927 1105 24-25 -2.8727268969456468 1105 26-27 -3.8535231406325146 1105 28-29 -5.438787991522361 1105 30-31 -5.595888009559879 1105 32-33 -8.019645299723422 1105 34-35 -4.019137806637811 1105 36-37 -3.4353877314814874 1105 38-39 -6.051103670634912 1105 40-41 -7.394594381313134 1105 42-43 -1.5680268082611803 1105 44-45 -3.663456176346795 1105 46-47 -3.5190827546296326 1105 48-49 -3.614679909211162 1105 50-51 -5.63947510822511 1105 52-53 -0.8159402807840301 1105 54-55 -4.1674918831168775 1105 56-57 -7.69714481120732 1105 58-59 -1.7260292658730272 1105 60-61 -2.8037072736291364 1105 62-63 -1.7556128622534821 1105 64-65 -1.4118649290524345 1105 66-67 -7.053337692400188 1105 68-69 -5.292605932840303 1105 70-71 -3.659020825817702 1105 72-73 -2.2691503953222742 1105 74-75 -6.5959522682179035 1105 76-77 -6.883608781265043 1105 78-79 -8.56717509920634 1105 80-81 -3.612891376563251 1105 82-83 -3.259993912337663 1105 84-85 -2.887041922198172 1105 86-87 -4.670699179292939 1105 88-89 -5.840639091810967 1105 90-91 -5.52338207221019 1105 92-93 -5.478300490019244 1105 94-95 -6.205516850048092 1105 96-97 -10.02314777236653 1105 98-99 -8.759808651996147 1105 100 -8.819421897546906 1106 1 5.706401815776822 1106 2 5.6110851821789325 1106 3 -0.33992830086580383 1106 4 2.6983059764309836 1106 5 -2.528774350649364 1106 6 6.281245114838864 1106 7 7.334303977272725 1106 8 7.364107969576718 1106 9 5.139025297619039 1106 10-11 5.246484938672438 1106 12-13 6.107205800565172 1106 14-15 2.6380903529341175 1106 16-17 3.897919672919688 1106 18-19 3.2835490319865244 1106 20-21 -1.5891333348364611 1106 22-23 3.490969215969219 1106 24-25 1.1558445316257853 1106 26-27 2.246476859367487 1106 28-29 0.7612120084776386 1106 30-31 3.189826276154406 1106 32-33 -0.31964529972341893 1106 34-35 0.6951479076479039 1106 36-37 0.5646122685185126 1106 38-39 0.8774677579365182 1106 40-41 2.4482627615440116 1106 42-43 -0.7965982368326081 1106 44-45 -0.634884747775363 1106 46-47 1.780917245370368 1106 48-49 -0.7432513377825956 1106 50-51 5.0748106060606055 1106 52-53 5.312631147787403 1106 54-55 3.3039366883116905 1106 56-57 2.588569474506965 1106 58-59 -2.8831721230158784 1106 60-61 4.7391498692280045 1106 62-63 0.15867285203223247 1106 64-65 -1.3975792147667185 1106 66-67 1.8609480218855303 1106 68-69 2.050251210016839 1106 70-71 2.669550602753727 1106 72-73 0.8737067475348681 1106 74-75 3.8183334460678147 1106 76-77 6.31639121873496 1106 78-79 3.804253472222232 1106 80-81 1.715680052008178 1106 82-83 1.1971489448051962 1106 84-85 2.2558152206589703 1106 86-87 -0.5421277507215088 1106 88-89 7.602218051046179 1106 90-91 0.2051893563612346 1106 92-93 2.378842367123614 1106 94-95 1.9801974356661916 1106 96-97 0.9482807990620401 1106 98-99 2.9259056337181377 1106 100 5.980578102453094 1107 1 2.9397351491101524 1107 2 3.211085182178934 1107 3 4.493405032467528 1107 4 3.76497264309765 1107 5 3.9378923160173045 1107 6 6.6145784481722 1107 7 5.534303977272728 1107 8 5.364107969576718 1107 9 5.23902529761904 1107 10-11 7.379818272005771 1107 12-13 6.990539133898498 1107 14-15 7.8214236862674476 1107 16-17 8.481253006253024 1107 18-19 5.950215698653189 1107 20-21 5.69419999849687 1107 22-23 8.24096921596922 1107 24-25 8.222511198292455 1107 26-27 6.813143526034157 1107 28-29 10.144545341810968 1107 30-31 8.606492942821077 1107 32-33 7.730354700276578 1107 34-35 7.2784812409812325 1107 36-37 7.064612268518513 1107 38-39 8.627467757936518 1107 40-41 6.081596094877344 1107 42-43 6.503401763167393 1107 44-45 5.9317819188913 1107 46-47 6.730917245370367 1107 48-49 6.623415328884079 1107 50-51 5.408143939393938 1107 52-53 7.3626311477874005 1107 54-55 5.7372700216450205 1107 56-57 8.171902807840294 1107 58-59 6.416827876984119 1107 60-61 6.422483202561342 1107 62-63 8.542006185365562 1107 64-65 8.30242078523328 1107 66-67 9.827614688552199 1107 68-69 7.35025121001684 1107 70-71 7.9362172694204 1107 72-73 4.707040080868204 1107 74-75 7.868333446067812 1107 76-77 8.699724552068293 1107 78-79 8.504253472222231 1107 80-81 7.0656800520081795 1107 82-83 5.9304822781385305 1107 84-85 6.672481887325638 1107 86-87 8.67453891594516 1107 88-89 6.802218051046179 1107 90-91 7.405189356361237 1107 92-93 7.712175700456946 1107 94-95 7.163530768999529 1107 96-97 6.2982807990620415 1107 98-99 6.275905633718139 1107 100 3.3472447691197615 1108 1 -2.8935981842231797 1108 2 3.044418515512266 1108 3 -6.839928300865804 1108 4 -2.23502735690235 1108 5 -1.0621076839826955 1108 6 -1.885421551827804 1108 7 -3.965696022727272 1108 8 -4.30255869708995 1108 9 -1.5943080357142918 1108 10-11 -3.4535150613275647 1108 12-13 -7.176127532768167 1108 14-15 -1.3452429803992167 1108 16-17 -0.7687469937469764 1108 18-19 -0.13311763468013993 1108 20-21 -1.3058000015031261 1108 22-23 1.8243025493025549 1108 24-25 -3.527488801707552 1108 26-27 -5.18685647396585 1108 28-29 -1.355454658189025 1108 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2.5482807990620415 2312 98-99 6.025905633718139 2312 100 0.380578102453093 2313 1 -2.2935981842231783 2313 2 -4.988914817821065 2313 3 -0.5399283008658031 2313 4 -13.101694023569017 2313 5 -4.528774350649364 2313 6 -8.718754885161136 2313 7 -12.265696022727273 2313 8 -0.23589203042328322 2313 9 -5.860974702380961 2313 10-11 -0.2535150613275654 2313 12-13 -12.29279419943483 2313 14-15 -9.761909647065881 2313 16-17 -7.7020803270803135 2313 18-19 -3.916450968013475 2313 20-21 -5.48913333483646 2313 22-23 -10.70903078403078 2313 24-25 -9.444155468374216 2313 26-27 -9.553523140632514 2313 28-29 -12.63878799152236 2313 30-31 -9.910173723845595 2313 32-33 -7.019645299723422 2313 34-35 -8.904852092352098 2313 36-37 -12.335387731481488 2313 38-39 -10.322532242063485 2313 40-41 -9.851737238455993 2313 42-43 -11.996598236832607 2313 44-45 -10.534884747775365 2313 46-47 -5.81908275462963 2313 48-49 -13.543251337782593 2313 50-51 -10.525189393939394 2313 52-53 -7.087368852212602 2313 54-55 -11.196063311688308 2313 56-57 -4.611430525493038 2313 58-59 -8.28317212301588 2313 60-61 -13.460850130771995 2313 62-63 -9.341327147967768 2313 64-65 -8.897579214766719 2313 66-67 -11.739051978114473 2313 68-69 -4.449748789983158 2313 70-71 -11.830449397246271 2313 72-73 -4.826293252465131 2313 74-75 -10.081666553932187 2313 76-77 -7.983608781265044 2313 78-79 -7.895746527777767 2313 80-81 -10.78431994799182 2313 82-83 -9.502851055194805 2313 84-85 -5.044184779341027 2313 86-87 -8.742127750721508 2313 88-89 -7.097781948953822 2313 90-91 -10.794810643638762 2313 92-93 -11.521157632876385 2313 94-95 -7.119802564333806 2313 96-97 -9.651719200937958 2313 98-99 -5.17409436628186 2313 100 -6.619421897546907 2314 1 1.7730684824434881 2314 2 2.544418515512266 2314 3 -0.7843727453102467 2314 4 -6.123916245791239 2314 5 -2.7287743506493634 2314 6 -1.8298659962722468 2314 7 -0.9101404671717148 2314 8 -1.969225363756614 2314 9 -1.5943080357142918 2314 10-11 -1.2312928391053433 2314 12-13 0.018316911676283354 2314 14-15 -3.5396874248436596 2314 16-17 0.8979196729196879 2314 18-19 -2.0497843013468113 2314 20-21 -2.3891333348364583 2314 22-23 -1.9534752284752237 2314 24-25 -2.6663776905964376 2314 26-27 -2.075745362854736 2314 28-29 0.33898978625541787 2314 30-31 -1.365729279401151 2314 32-33 1.1470213669432425 2314 34-35 -4.0270743145743175 2314 36-37 -1.6576099537037088 2314 38-39 -2.7336433531745925 2314 40-41 -3.7517372384559913 2314 42-43 -3.107709347943718 2314 44-45 -4.179329192219807 2314 46-47 -3.407971643518522 2314 48-49 -7.209918004449257 2314 50-51 -6.591856060606062 2314 52-53 -3.165146629990378 2314 54-55 -3.9849522005771973 2314 56-57 -5.078097192159699 2314 58-59 -5.194283234126992 2314 60-61 -4.771961241883105 2314 62-63 -5.096882703523324 2314 64-65 -3.530912548100055 2314 66-67 -4.39460753367003 2314 68-69 -1.9830821233164961 2314 70-71 -3.6748938416907144 2314 72-73 -1.8485154746873533 2314 74-75 -2.7149998872655203 2314 76-77 -1.050275447931707 2314 78-79 -1.162413194444433 2314 80-81 -4.239875503547378 2314 82-83 -1.1250732774170267 2314 84-85 -7.021962557118808 2314 86-87 -3.0199055284992866 2314 88-89 -4.864448615620489 2314 90-91 -1.9836995325276519 2314 92-93 -2.232268743987497 2314 94-95 -2.2531358976671427 2314 96-97 -5.340608089826848 2314 98-99 -2.1407610329485287 2314 100 -1.7083107864357956 2315 1 2.6064018157768203 2315 2 -0.2889148178210661 2315 3 -3.339928300865804 2315 4 4.348305976430982 2315 5 7.271225649350637 2315 6 6.531245114838864 2315 7 0.534303977272728 2315 8 3.114107969576718 2315 9 -6.51097470238096 2315 10-11 0.2964849386724353 2315 12-13 -1.217794199434831 2315 14-15 -5.011909647065881 2315 16-17 1.0229196729196879 2315 18-19 0.5335490319865244 2315 20-21 -0.014133334836458289 2315 22-23 1.8659692159692192 2315 24-25 -0.06915546837421616 2315 26-27 2.5214768593674854 2315 28-29 -4.438787991522361 2315 30-31 0.31482627615440606 2315 32-33 0.8553547002765782 2315 34-35 5.320147907647904 2315 36-37 -1.6853877314814874 2315 38-39 -1.6225322420634818 2315 40-41 3.4982627615440087 2315 42-43 -1.2465982368326074 2315 44-45 1.0151152522246356 2315 46-47 -6.769082754629633 2315 48-49 -4.668251337782593 2315 50-51 1.8248106060606055 2315 52-53 -0.7623688522125995 2315 54-55 -3.596063311688308 2315 56-57 -2.286430525493035 2315 58-59 -2.0831721230158813 2315 60-61 -4.410850130771994 2315 62-63 -2.416327147967767 2315 64-65 -3.0725792147667192 2315 66-67 -10.089051978114473 2315 68-69 -1.6497487899831604 2315 70-71 -1.6054493972462716 2315 72-73 2.123706747534868 2315 74-75 0.24333344606781182 2315 76-77 -2.5086087812650426 2315 78-79 -1.8707465277777686 2315 80-81 -1.8093199479918205 2315 82-83 -0.40285105519480524 2315 84-85 -0.6191847793410297 2315 86-87 -1.242127750721508 2315 88-89 2.9272180510461787 2315 90-91 -2.4698106436387626 2315 92-93 -2.496157632876386 2315 94-95 -2.669802564333807 2315 96-97 -0.5767192009379585 2315 98-99 -3.099094366281861 2315 100 -1.5694218975469063 2316 1 -0.8935981842231797 2316 2 -5.588914817821067 2316 3 -9.339928300865804 2316 4 -4.901694023569018 2316 5 -10.328774350649365 2316 6 -4.118754885161135 2316 7 -6.465696022727272 2316 8 -6.03589203042328 2316 9 -6.06097470238096 2316 10-11 -7.253515061327562 2316 12-13 -3.3927941994348316 2316 14-15 -6.16190964706588 2316 16-17 -4.402080327080313 2316 18-19 -4.516450968013476 2316 20-21 -5.389133334836458 2316 22-23 -3.309030784030778 2316 24-25 -0.24415546837421687 2316 26-27 -5.053523140632514 2316 28-29 -6.738787991522358 2316 30-31 -7.2101737238455925 2316 32-33 -7.819645299723419 2316 34-35 -4.4048520923520975 2316 36-37 -7.435387731481487 2316 38-39 -2.622532242063482 2316 40-41 -4.65173723845599 2316 42-43 -7.796598236832608 2316 44-45 -0.8348847477753658 2316 46-47 -9.119082754629634 2316 48-49 -9.943251337782591 2316 50-51 -2.2251893939393916 2316 52-53 -3.1873688522126002 2316 54-55 -7.096063311688308 2316 56-57 -5.911430525493035 2316 58-59 -10.283172123015884 2316 60-61 -11.560850130771994 2316 62-63 -10.641327147967768 2316 64-65 -8.49757921476672 2316 66-67 -7.8390519781144725 2316 68-69 -7.64974878998316 2316 70-71 -2.930449397246271 2316 72-73 -2.626293252465132 2316 74-75 -2.4816665539321896 2316 76-77 -4.783608781265041 2316 78-79 -6.995746527777769 2316 80-81 -10.18431994799182 2316 82-83 -11.502851055194805 2316 84-85 -9.84418477934103 2316 86-87 -2.942127750721511 2316 88-89 -5.59778194895382 2316 90-91 -4.894810643638763 2316 92-93 -5.721157632876388 2316 94-95 -8.219802564333808 2316 96-97 -5.851719200937957 2316 98-99 -12.87409436628186 2316 100 -10.419421897546906 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 11.0 1 2.0 2 2.0 3 0.0 4 1.0 5 1.0 6 1.0 7 16.0 8 61.0 9 145.0 10 170.0 11 265.0 12 231.0 13 230.0 14 209.0 15 160.0 16 191.0 17 170.0 18 190.0 19 169.0 20 165.0 21 172.0 22 132.0 23 129.0 24 127.0 25 136.0 26 123.0 27 139.0 28 131.0 29 149.0 30 207.0 31 222.0 32 223.0 33 286.0 34 369.0 35 435.0 36 456.0 37 511.0 38 400.0 39 70.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.791053560918186 17.67216009417304 13.18422601530312 24.352560329605648 2 16.973354924186662 22.037391432356838 36.44928602973649 24.539967613720005 3 21.27377266696152 24.576146247972876 28.674627745835174 25.47545333923043 4 14.871039056742816 16.38909358879882 35.71112748710391 33.02873986735445 5 15.317706029780334 36.561993218339964 31.800088456435205 16.320212295444495 6 30.108951707891634 34.849823321554766 18.227326266195522 16.813898704358067 7 27.460223924572773 31.437831467295226 21.022392457277547 20.07955215085445 8 25.489618612869975 32.18966278898542 20.39463996465911 21.926078633485496 9 27.161584916777137 16.18795109736338 19.67889232582118 36.9715716600383 10-11 25.4860088365243 24.432989690721648 27.65095729013255 22.430044182621504 12-13 26.95876288659794 22.81296023564065 26.54639175257732 23.681885125184092 14-15 24.099845372211178 24.23238347691628 24.740446211619176 26.927324939253367 16-17 23.2960400412189 25.96790814073311 25.489474459001915 25.24657735904608 18-19 22.857773851590103 26.479681978798585 26.98763250883392 23.674911660777383 20-21 24.884012077472565 24.736725826644083 26.018116208851904 24.361145887031448 22-23 23.93017603299698 25.093908816380644 26.154526036679677 24.821389113942697 24-25 24.668630338733433 24.8379970544919 26.296023564064804 24.197349042709867 26-27 24.791988807893382 24.468006774169794 27.288123113172812 23.451881304764008 28-29 23.655913978494624 25.018412137280894 26.99955810870526 24.326115775519224 30-31 24.441988950276244 24.913443830570902 26.7182320441989 23.92633517495396 32-33 24.48258083523606 25.2412167636444 26.397584149664876 23.878618251454668 34-35 24.615271334953242 25.138060525734485 26.654885501803992 23.59178263750828 36-37 23.875101259297445 25.252227704543778 26.673540025038662 24.199131011120112 38-39 24.177038073495837 25.517342956035055 26.42315339863024 23.882465571838868 40-41 24.21719590363221 25.27075812274368 25.8896338318721 24.622412141752008 42-43 24.00942701428782 25.349830608337015 25.70334364413021 24.937398733244954 44-45 24.493852609879994 24.95766767282633 25.966281381138188 24.58219833615549 46-47 25.21540614183666 25.451064143162235 24.71463288901981 24.618896825981295 48-49 23.96316758747698 25.43646408839779 25.930018416206263 24.67034990791897 50-51 23.379970544918997 25.67746686303387 26.730486008836525 24.212076583210603 52-53 23.620016213427668 26.405777876040975 25.24872871987619 24.72547719065517 54-55 22.780520150298386 25.138141899359024 26.191704118470494 25.8896338318721 56-57 23.34733583904488 25.071854963519787 26.951138624806543 24.629670572628786 58-59 24.368324125230203 24.95027624309392 25.43646408839779 25.244935543278086 60-61 24.167648791985858 25.567177371832646 26.39216263995286 23.87301119622864 62-63 23.476979742173114 25.377532228360955 26.813996316758747 24.33149171270718 64-65 23.18573638841818 26.56008251676122 25.30022839460694 24.953952700213662 66-67 23.775502688370036 25.528467260808718 24.953966266480077 25.742063784341163 68-69 22.59966104192764 25.3629061970378 25.85660599808415 26.18082676295041 70-71 23.750552852720038 24.708830900781365 26.116762494471473 25.423853752027124 72-73 23.47954294139329 25.175082934021383 26.14817545152967 25.19719867305566 74-75 22.982236308690204 25.724183680990638 26.01901673177563 25.27456327854353 76-77 23.526377836722663 24.84526967285588 25.707338638373123 25.921013852048336 78-79 22.843462246777165 25.620626151012893 25.517495395948437 26.018416206261506 80-81 23.26849395814913 25.677866195107573 25.095785440613028 25.95785440613027 82-83 23.903912755139636 24.906049664726254 25.738707538132783 25.451330042001324 84-85 22.97546238302262 25.66502100066318 25.20079581460467 26.158720801709528 86-87 23.46479911537044 26.28086988573535 25.602653888684113 24.651677110210098 88-89 23.28100818041123 26.074139582872725 25.300316898813474 25.344535337902574 90-91 24.23751289229409 25.077353764549876 25.01105053779284 25.674082805363195 92-93 23.949889462048638 25.32792925571113 25.077376565954314 25.644804716285925 94-95 23.72144436256448 25.13633014001474 25.65217391304348 25.490051584377305 96-97 23.266268700714864 25.03500626427887 26.38366865649643 25.315056378509837 98-99 23.835838491010904 25.346301208370175 25.198938992042443 25.618921308576482 100 23.728813559322035 24.64259395725866 25.217391304347824 26.41120117907148 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 10.0 1 6.5 2 3.0 3 2.5 4 1.5 5 1.5 6 2.5 7 2.5 8 1.5 9 2.0 10 4.0 11 3.5 12 3.5 13 5.0 14 5.0 15 4.0 16 5.0 17 5.5 18 6.5 19 8.5 20 5.0 21 4.5 22 9.5 23 14.5 24 16.5 25 15.0 26 18.0 27 26.5 28 29.0 29 31.5 30 44.5 31 59.0 32 76.0 33 86.0 34 90.0 35 101.5 36 110.0 37 130.0 38 141.0 39 141.0 40 148.5 41 152.0 42 161.0 43 178.5 44 201.5 45 225.0 46 238.0 47 254.0 48 282.5 49 288.0 50 280.0 51 267.0 52 254.5 53 244.0 54 243.5 55 243.0 56 234.5 57 225.0 58 199.5 59 177.5 60 155.5 61 139.0 62 123.0 63 106.5 64 92.5 65 76.5 66 67.0 67 56.5 68 43.5 69 35.5 70 31.0 71 33.0 72 29.5 73 16.5 74 14.5 75 15.0 76 11.5 77 8.5 78 5.5 79 5.5 80 8.0 81 4.5 82 0.5 83 1.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16159835463493463 2 0.205670633171735 3 0.35257822829440283 4 0.32319670926986926 5 0.35257822829440283 6 0.22036139268400176 7 0.2791244307330689 8 0.23505215219626852 9 0.2644336712208021 10-11 0.24974291170853533 12-13 0.24974291170853533 14-15 0.24239753195240193 16-17 0.205670633171735 18-19 0.22036139268400176 20-21 0.2570882914646687 22-23 0.2717790509769355 24-25 0.24974291170853533 26-27 0.24239753195240193 28-29 0.2644336712208021 30-31 0.2864698104892023 32-33 0.2717790509769355 34-35 0.24239753195240193 36-37 0.2570882914646687 38-39 0.2570882914646687 40-41 0.30116057000146906 42-43 0.2644336712208021 44-45 0.22770677244013515 46-47 0.2570882914646687 48-49 0.2864698104892023 50-51 0.24974291170853533 52-53 0.3305420890260027 54-55 0.30116057000146906 56-57 0.3305420890260027 58-59 0.2864698104892023 60-61 0.2791244307330689 62-63 0.2864698104892023 64-65 0.30116057000146906 66-67 0.2717790509769355 68-69 0.31585132951373585 70-71 0.35257822829440283 72-73 0.3599236080505362 74-75 0.3452328485382694 76-77 0.3085059497576025 78-79 0.2864698104892023 80-81 0.3085059497576025 82-83 0.31585132951373585 84-85 0.31585132951373585 86-87 0.3599236080505362 88-89 0.3305420890260027 90-91 0.2938151902453357 92-93 0.32319670926986926 94-95 0.32319670926986926 96-97 0.3305420890260027 98-99 0.3085059497576025 100 0.32319670926986926 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6807.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.70824151608637 #Duplication Level Percentage of deduplicated Percentage of total 1 99.27830401443391 97.00308505949758 2 0.40595399188092013 0.7933010136624064 3 0.07517666516313336 0.22036139268400176 4 0.030070666065253347 0.11752607609813426 5 0.04510599909788002 0.22036139268400176 6 0.04510599909788002 0.2644336712208021 7 0.04510599909788002 0.3085059497576025 8 0.015035333032626674 0.11752607609813426 9 0.0 0.0 >10 0.060141332130506694 0.9548993682973409 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 32 0.47010430439253703 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.1909798736594682 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 10 0.14690759512266785 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 10 0.14690759512266785 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.11752607609813426 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 7 0.1028353165858675 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7 0.1028353165858675 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7 0.1028353165858675 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.03672689878066696 0.0 32-33 0.0 0.0 0.0 0.058763038049067136 0.0 34-35 0.0 0.0 0.0 0.08814455707360071 0.0 36-37 0.0 0.0 0.0 0.12487145585426768 0.0 38-39 0.0 0.0 0.0 0.16159835463493463 0.0 40-41 0.0 0.0 0.0 0.24974291170853535 0.0 42-43 0.0 0.0 0.0 0.3085059497576025 0.0 44-45 0.0 0.0 0.0 0.35992360805053625 0.0 46-47 0.0 0.0 0.0 0.46275892463640367 0.0 48-49 0.0 0.0 0.0 0.5729396209784046 0.0 50-51 0.0 0.0 0.0 0.6610841780520054 0.0 52-53 0.0 0.0 0.0 0.8373732921992068 0.0 54-55 0.0 0.0 0.0 1.0356985456148082 0.0 56-57 0.0 0.0 0.0 1.25605993829881 0.0 58-59 0.0 0.0 0.0 1.4690759512266784 0.0 60-61 0.0 0.0 0.0 1.6600558248861466 0.0 62-63 0.0 0.0 0.0 1.7922726604965478 0.0 64-65 0.0 0.0 0.0 1.9759071543998825 0.0 66-67 0.0 0.0 0.0 2.1889231673277507 0.0 68-69 0.0 0.0 0.0 2.4092845600117525 0.0 70-71 0.0 0.0 0.0 2.6590274717202877 0.0 72-73 0.0 0.0 0.0 2.9087703834288234 0.0 74-75 0.0 0.0 0.0 3.2099309534302924 0.0 76-77 0.0 0.0 0.0 3.400910827089761 0.0 78-79 0.0 0.0 0.0 3.6359629792860293 0.0 80-81 0.0 0.0 0.0 3.9297781695313647 0.0 82-83 0.0 0.0 0.0 4.275011018069634 0.0 84-85 0.0 0.0 0.0 4.730424562949905 0.0 86-87 0.0 0.0 0.0 5.097693550756574 0.0 88 0.0 0.0 0.0 5.362127221977376 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACAT 15 6.6084263E-4 94.0 7 GTACATG 20 0.0020714246 70.49999 8 TACATGG 20 0.0020714246 70.49999 9 GAGTACA 25 0.005015639 56.399998 6 >>END_MODULE