FastQCFastQC Report
Mon 6 Jun 2016
ERR522819_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522819_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5692124
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269240.4730044531707321No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219670.38591921047398126No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137750.242001052682619No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135640.23829417630395963No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT130350.22900063315556723No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61850.10865891185785834No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA59790.10503987615167905No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG107750.055.129912
GTACCTG124650.048.4623151
ACCTGGG121200.048.4298553
CCTGGGG105400.037.8810774
TATAACG5800.031.625532
CTGGGGG67050.027.281235
TATCACG5400.026.1293542
ATAACGC7250.025.3004253
GTACCCG10900.023.7392831
ATGGGGG87750.023.4178715
CATGGGG147250.023.2185084
TGGGGGG106050.022.5994076
TAACGCA8400.022.394224
GTACATG282350.021.7780631
GTACACG11150.021.5192281
TACATGG289500.020.990132
GTACCGG9900.020.4345321
ACATGGG292500.020.1477413
GAGTACT215950.019.55043812-13
TACCCGG12350.019.4224682
Produced by FastQC (version 0.11.5)