FastQCFastQC Report
Mon 6 Jun 2016
ERR522819_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522819_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5692124
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241820.4248326283826565No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213450.37499183081745935No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT139470.24502277181593374No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134500.23629140897141382No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121690.21378662868201748No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT69480.12206339847831846No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59730.10493446734470296No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA59610.10472364973075077No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG105800.055.929092
ACCTGGG118400.049.256163
GTACCTG125750.047.5977061
CCTGGGG105250.037.7736324
TATAACG6400.034.5156142
ATAACGC7900.029.7429053
TATCACG5400.029.592582
CTGGGGG62600.029.577565
TAACGCA8500.026.537674
TACCGTA5000.023.4968977
TGGGGGG93950.023.2592986
CATGGGG148150.021.8870834
GTACATG280450.021.2583431
GTACCGG9200.020.9537431
ATGGGGG90100.020.6021615
ACCGTAT6200.020.4700728
TACATGG287650.020.3097442
GAGTACT227600.020.29208612-13
ACATGGG288050.019.479463
GTACACG11650.019.3722881
Produced by FastQC (version 0.11.5)