##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR522818_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 41310 Sequences flagged as poor quality 0 Sequence length 100 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.30159767610748 31.0 26.0 34.0 11.0 34.0 2 27.433309126119585 31.0 25.0 34.0 10.0 35.0 3 27.162188332122973 31.0 23.0 34.0 10.0 36.0 4 29.182159283466472 34.0 26.0 37.0 10.0 37.0 5 28.976397966594046 34.0 24.0 37.0 10.0 37.0 6 29.40336480271121 35.0 25.0 37.0 10.0 37.0 7 29.31692084241104 34.0 25.0 37.0 10.0 37.0 8 29.340280803679498 35.0 25.0 37.0 10.0 37.0 9 30.324134592108447 35.0 25.0 39.0 10.0 39.0 10-11 30.1639070442992 34.5 25.0 39.0 10.0 39.0 12-13 30.22023723069475 35.5 25.0 39.0 10.0 39.0 14-15 30.677426773178407 36.0 25.0 40.0 9.5 41.0 16-17 30.634192689421447 36.5 24.5 39.5 10.0 41.0 18-19 30.493524570321956 35.5 23.5 40.0 8.5 41.0 20-21 30.655252965383685 36.0 24.5 40.0 8.0 41.0 22-23 30.586625514403295 36.0 24.0 40.0 8.0 41.0 24-25 30.528346647300893 36.0 24.0 40.0 8.0 41.0 26-27 30.318736383442264 36.5 23.0 40.0 8.0 41.0 28-29 30.118615347373517 35.5 22.0 39.0 8.0 41.0 30-31 29.902457032195592 36.0 21.0 39.0 8.0 41.0 32-33 29.696901476640036 35.5 20.5 39.0 8.0 41.0 34-35 29.684773662551443 35.0 21.0 39.0 8.0 41.0 36-37 29.656572258533043 35.0 21.5 39.0 8.0 41.0 38-39 29.523214717985958 35.5 20.0 39.0 8.0 41.0 40-41 29.446320503510044 35.5 20.0 39.0 8.0 41.0 42-43 29.244274993948196 36.0 19.5 38.5 8.0 41.0 44-45 29.122440087145968 35.0 19.5 38.5 8.0 40.0 46-47 28.85807310578552 35.0 19.5 38.0 8.0 40.0 48-49 28.719535221496006 34.5 19.5 38.0 8.0 40.0 50-51 28.107697893972404 34.0 18.5 37.5 8.0 39.0 52-53 28.460723795691116 34.5 20.0 37.5 8.0 39.5 54-55 29.228746066327766 35.0 20.0 38.5 8.0 40.5 56-57 29.289869281045753 35.5 19.5 39.0 8.0 41.0 58-59 29.265541031227308 35.0 19.5 39.0 8.0 41.0 60-61 28.965734688937303 35.0 18.0 39.0 7.5 41.0 62-63 28.647288792060035 34.5 18.5 38.5 7.0 40.0 64-65 28.417550229968533 34.5 18.0 38.0 7.0 40.0 66-67 28.17196804647785 34.0 18.0 37.0 7.0 40.0 68-69 27.925623335754054 34.0 18.0 37.0 7.0 39.5 70-71 27.63461631566207 34.0 18.0 37.0 7.0 39.0 72-73 27.2261316872428 33.0 16.5 36.0 7.0 39.0 74-75 26.881820382473975 33.0 17.0 36.0 7.0 37.5 76-77 26.593512466715083 32.5 18.0 35.5 7.0 37.5 78-79 26.2879690147664 32.5 17.0 35.5 7.0 37.0 80-81 26.041624304042607 32.0 16.5 35.0 7.0 37.0 82-83 25.753159041394333 32.0 15.5 35.0 7.0 36.5 84-85 25.492725732268216 32.0 13.5 35.0 7.0 36.0 86-87 25.195763737593804 31.0 12.5 35.0 7.0 36.5 88-89 24.996562575647545 31.0 11.0 35.0 7.0 36.0 90-91 24.75985233599613 30.0 11.0 35.0 7.0 36.0 92-93 24.315831517792304 30.0 10.0 35.0 7.0 35.0 94-95 24.204490438150568 30.0 10.0 35.0 7.0 35.0 96-97 24.065565238441053 29.5 10.0 34.5 7.0 35.0 98-99 23.850774630839993 29.0 10.0 35.0 7.0 35.0 100 22.682643427741468 26.0 10.0 34.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.9378530906422391 1101 2 0.4333614647866355 1101 3 0.4276661024259063 1101 4 1.170539723014432 1101 5 -0.19217608960019206 1101 6 -0.31327826867789454 1101 7 -0.23603675524030976 1101 8 0.3746644466801321 1101 9 -0.8159218572295188 1101 10-11 0.11830567380890145 1101 12-13 -0.78038574368545 1101 14-15 0.4223551735354931 1101 16-17 0.6741125067272016 1101 18-19 0.0822202267836758 1101 20-21 0.5991047894563444 1101 22-23 2.2261986900785224 1101 24-25 0.7019736613169272 1101 26-27 0.5856009967706655 1101 28-29 0.08319126449685754 1101 30-31 0.9412382007588036 1101 32-33 0.3495988698507304 1101 34-35 0.5350725802562408 1101 36-37 0.12954072489710455 1101 38-39 1.0440678612108023 1101 40-41 0.7491372873701003 1101 42-43 0.4484700221788458 1101 44-45 0.8527121190649396 1101 46-47 0.5555866399543525 1101 48-49 1.0557019876159934 1101 50-51 -0.24871643619561823 1101 52-53 0.4407368968868717 1101 54-55 0.7526901709103448 1101 56-57 1.2965682692577722 1101 58-59 0.9174382114491557 1101 60-61 0.8062878954393433 1101 62-63 0.33237354544946385 1101 64-65 0.8843651414948503 1101 66-67 1.3707947612128528 1101 68-69 1.5946312531341071 1101 70-71 0.9862854088294704 1101 72-73 -0.3762122820058984 1101 74-75 1.5811849653593129 1101 76-77 1.7353392283447526 1101 78-79 0.3793752002776998 1101 80-81 0.6839812571704371 1101 82-83 0.8058219015800923 1101 84-85 1.2843825851574024 1101 86-87 0.8463194295539438 1101 88-89 0.7007956184856532 1101 90-91 -0.9639445370616606 1101 92-93 0.5364926108181045 1101 94-95 0.3481753062862225 1101 96-97 0.28890036222539095 1101 98-99 0.02496382993077262 1101 100 0.010595939539062016 1102 1 -0.09334089552028857 1102 2 -1.445906827896291 1102 3 0.2173002487673692 1102 4 0.6741982595997982 1102 5 -0.601932187161168 1102 6 1.2897705118099125 1102 7 0.0779876350035913 1102 8 -1.693628236246699 1102 9 0.4609074110631646 1102 10-11 0.9173910396625651 1102 12-13 0.42601669533893727 1102 14-15 1.819916149145243 1102 16-17 1.666185677458909 1102 18-19 -0.5549748951675433 1102 20-21 0.958556008968543 1102 22-23 0.7902230803224235 1102 24-25 0.7809370759510728 1102 26-27 0.367613191892616 1102 28-29 0.38075224010661657 1102 30-31 0.7964211275880722 1102 32-33 1.2721598454604859 1102 34-35 1.143304287573315 1102 36-37 0.5859431639214918 1102 38-39 -0.020566285130659168 1102 40-41 -0.7453749077518488 1102 42-43 -0.2637250997723761 1102 44-45 1.292346265406401 1102 46-47 0.16686712775922885 1102 48-49 0.365458085176968 1102 50-51 0.5473201491702326 1102 52-53 0.6748832383502865 1102 54-55 1.4572633416420544 1102 56-57 1.4642511960870408 1102 58-59 1.4470113821808646 1102 60-61 0.41909277348812424 1102 62-63 0.7323735454494624 1102 64-65 1.0831456292997252 1102 66-67 0.7467093953591935 1102 68-69 1.726643448256059 1102 70-71 2.675919555170932 1102 72-73 1.064031620433127 1102 74-75 0.019904477554433697 1102 76-77 1.1560709356618268 1102 78-79 1.0818142246679443 1102 80-81 0.9312373547314117 1102 82-83 0.7161877552386322 1102 84-85 0.3380411217427657 1102 86-87 -0.3506317899582534 1102 88-89 1.2437834233637055 1102 90-91 0.7119700970846772 1102 92-93 5.17001062004141E-4 1102 94-95 0.2978704282374416 1102 96-97 2.0361564597863655 1102 98-99 1.4676467567600398 1102 100 0.9916935005146748 1103 1 2.312146909357761 1103 2 2.083361464786634 1103 3 0.6526661024259077 1103 4 1.77053972301443 1103 5 2.8578239103998087 1103 6 1.9867217313221062 1103 7 1.9889632447596917 1103 8 2.4496644466801314 1103 9 1.8340781427704798 1103 10-11 2.4433056738089043 1103 12-13 2.28211425631455 1103 14-15 1.7598551735354917 1103 16-17 3.0991125067271987 1103 18-19 1.9947202267836772 1103 20-21 2.4866047894563437 1103 22-23 2.813698690078521 1103 24-25 2.08947366131693 1103 26-27 2.7856009967706683 1103 28-29 2.308191264496866 1103 30-31 1.5912382007588022 1103 32-33 2.2995988698507297 1103 34-35 1.8850725802562422 1103 36-37 2.4420407248971046 1103 38-39 2.556567861210805 1103 40-41 1.5616372873701039 1103 42-43 2.0609700221788465 1103 44-45 2.7152121190649403 1103 46-47 3.393086639954351 1103 48-49 3.0057019876159927 1103 50-51 2.6012835638043796 1103 52-53 2.6782368968868724 1103 54-55 2.1901901709103484 1103 56-57 2.8965682692577666 1103 58-59 3.71743821144916 1103 60-61 3.3062878954393433 1103 62-63 1.8323735454494638 1103 64-65 3.096865141494849 1103 66-67 2.270794761212855 1103 68-69 2.719631253134107 1103 70-71 1.7112854088294682 1103 72-73 1.4612877179941037 1103 74-75 2.2811849653593086 1103 76-77 2.385339228344751 1103 78-79 2.8668752002777005 1103 80-81 1.9839812571704378 1103 82-83 1.3558219015800965 1103 84-85 2.534382585157399 1103 86-87 1.496319429553946 1103 88-89 0.6132956184856546 1103 90-91 1.5235554629383365 1103 92-93 3.823992610818106 1103 94-95 2.9106753062862225 1103 96-97 1.688900362225393 1103 98-99 2.799963829930771 1103 100 2.3605959395390634 1104 1 0.4371469093577609 1104 2 0.3833614647866348 1104 3 0.9617570115168164 1104 4 0.9796306321053407 1104 5 0.9396420922179907 1104 6 0.22990354950392344 1104 7 0.5935086993051435 1104 8 0.05193717395285802 1104 9 0.5340781427704826 1104 10-11 0.3921693101725374 1104 12-13 0.19347789267818527 1104 14-15 1.1087188098991305 1104 16-17 1.5082034158181052 1104 18-19 0.661765681329129 1104 20-21 0.38205933491089183 1104 22-23 0.8398350537148858 1104 24-25 1.5190191158623776 1104 26-27 1.3071919058615755 1104 28-29 0.6695549008604971 1104 30-31 0.8446472916678935 1104 32-33 1.072326142578003 1104 34-35 0.3816634893471509 1104 36-37 1.4670407248971031 1104 38-39 0.30315877030171023 1104 40-41 0.20141001464282837 1104 42-43 -0.2401663414575168 1104 44-45 1.9686212099740281 1104 46-47 1.4476320944998058 1104 48-49 0.8238838057978128 1104 50-51 0.6160562910771077 1104 52-53 1.8021005332505062 1104 54-55 1.912917443637621 1104 56-57 1.7397500874395924 1104 58-59 2.1185745750855176 1104 60-61 1.81765153180298 1104 62-63 1.823282636358556 1104 64-65 0.977546959676669 1104 66-67 0.828749306667401 1104 68-69 1.6662221622250186 1104 70-71 1.3635581361021956 1104 72-73 1.2646968089031922 1104 74-75 1.1482304199047633 1104 76-77 0.9285210465265727 1104 78-79 0.25892065482315374 1104 80-81 -0.4057914701022902 1104 82-83 0.9603673561255484 1104 84-85 1.3707462215210384 1104 86-87 0.737228520463038 1104 88-89 0.008750163940202782 1104 90-91 0.6917372811201581 1104 92-93 0.8342198835453765 1104 94-95 1.1834025790134959 1104 96-97 1.2332185440435737 1104 98-99 0.9476911026580446 1104 100 -0.014404060460936563 1105 1 1.0359841186600853 1105 2 0.10429169734477384 1105 3 0.17475912568172092 1105 4 1.006586234642338 1105 5 2.0775913522602707 1105 6 1.1791635917872227 1105 7 -1.3610367552403098 1105 8 -0.6811495068082394 1105 9 -0.39615441536905394 1105 10-11 -0.305240837819003 1105 12-13 0.08909100050059493 1105 14-15 -0.10322622181334751 1105 16-17 0.2444613439365 1105 18-19 1.5153597616673977 1105 20-21 0.8482326964330902 1105 22-23 0.16515217845061514 1105 24-25 -0.11866587356679759 1105 26-27 0.4969382060729899 1105 28-29 -0.04239013085197385 1105 30-31 -0.05440133412491832 1105 32-33 -0.04633136270740934 1105 34-35 -0.22510183834841158 1105 36-37 0.3652965388505933 1105 38-39 1.6149980937689428 1105 40-41 0.5555326362073139 1105 42-43 -0.6585067220071998 1105 44-45 0.7265493283672626 1105 46-47 0.5134354771636538 1105 48-49 -0.5233677798258682 1105 50-51 0.5748300754322884 1105 52-53 0.7151555015380353 1105 54-55 1.5006552871894172 1105 56-57 0.47389385065311984 1105 58-59 -0.3816896955275908 1105 60-61 1.5940785931137604 1105 62-63 -0.10483575687612046 1105 64-65 1.6390163042855477 1105 66-67 0.6437598774919238 1105 68-69 0.5187591601108537 1105 70-71 1.2729133158062105 1105 72-73 0.9644853924127048 1105 74-75 -0.2568964299895242 1105 76-77 0.21789736787963676 1105 78-79 1.8072821770218859 1105 80-81 0.7400859083332278 1105 82-83 0.7819846922777707 1105 84-85 0.5774058409713554 1105 86-87 0.03876129001906392 1105 88-89 0.044690967322864594 1105 90-91 -0.007258490550032803 1105 92-93 1.4219577270971762 1105 94-95 0.5647450737280835 1105 96-97 1.193842222690506 1105 98-99 0.04705685318658581 1105 100 1.305363381399527 1106 1 -1.2176149954041442 1106 2 -1.1404480590228907 1106 3 -1.1259053261455207 1106 4 -0.7032698007950948 1106 5 -1.825509422933525 1106 6 -0.3739925543921778 1106 7 -0.67056056476412 1106 8 -1.949145077129394 1106 9 0.7483638570561979 1106 10-11 -0.1310990880958549 1106 12-13 -0.698838124637831 1106 14-15 -2.182406731226415 1106 16-17 -1.7223160647013707 1106 18-19 -1.5903988208353717 1106 20-21 -1.4967285438769906 1106 22-23 -1.8273727384929082 1106 24-25 -1.6081453863021231 1106 26-27 -1.0185656698959988 1106 28-29 -0.24061825931266512 1106 30-31 -1.6153094182888168 1106 32-33 -1.253972558720701 1106 34-35 -1.4917131340294745 1106 36-37 -1.717483084626707 1106 38-39 -1.1059321387891963 1106 40-41 -1.7520531888203728 1106 42-43 -0.9658156921068688 1106 44-45 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68-69 0.39349488949774525 1107 70-71 1.761285408829469 1107 72-73 1.7874240816304656 1107 74-75 1.6595940562684035 1107 76-77 2.6217028647083893 1107 78-79 1.9066479275504271 1107 80-81 1.28739034807953 1107 82-83 1.062640083398275 1107 84-85 1.8480189487937686 1107 86-87 2.373592156826671 1107 88-89 3.406477436667476 1107 90-91 3.1121918265747013 1107 92-93 1.8455835199090131 1107 94-95 2.4561298517407693 1107 96-97 3.721854907679937 1107 98-99 2.3681456481125878 1107 100 1.5992323031754268 1108 1 -2.5378530906422405 1108 2 -0.7916385352133659 1108 3 -0.9973338975740944 1108 4 -0.9294602769855693 1108 5 0.35782391039980865 1108 6 -0.18827826867789454 1108 7 -1.311036755240309 1108 8 -0.5503355533198686 1108 9 -0.6409218572295181 1108 10-11 -1.9191943261910929 1108 12-13 -0.13038574368545142 1108 14-15 -0.20264482646450688 1108 16-17 -2.5008874932728027 1108 18-19 -1.3552797732163242 1108 20-21 -1.1258952105436535 1108 22-23 -3.5113013099214783 1108 24-25 -1.973026338683077 1108 26-27 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2311 62-63 0.5776116406875573 2311 64-65 0.9272222843519948 2311 66-67 0.3904376183557119 2311 68-69 1.554750300753156 2311 70-71 1.4041425516866113 2311 72-73 0.757121051327438 2311 74-75 0.9853516320259743 2311 76-77 1.7353392283447526 2311 78-79 1.2627085336110362 2311 80-81 1.1310050666942466 2311 82-83 2.0236790444372375 2311 84-85 0.9605730613478762 2311 86-87 1.568938477172992 2311 88-89 1.5228194280094662 2311 90-91 1.3914126057954803 2311 92-93 2.0853021346276286 2311 94-95 2.08329435390527 2311 96-97 2.5930670288920545 2311 98-99 1.6987733537402967 2311 100 2.7355959395390634 2312 1 1.0507832729941242 2312 2 0.8151796466048182 2312 3 1.029938829698633 2312 4 0.684176086650794 2312 5 0.8941875467634439 2312 6 0.8889944585948335 2312 7 -0.2019458461493997 2312 8 0.2564826284983148 2312 9 0.9886235973159359 2312 10-11 0.9035329465361777 2312 12-13 0.45484152904182196 2312 14-15 0.6200824462627637 2312 16-17 -0.09406931145462138 2312 18-19 0.3322202267836758 2312 20-21 0.9616047894563451 2312 22-23 0.10119869007852245 2312 24-25 1.200837297680561 2312 26-27 0.22764645131611871 2312 28-29 1.1809185372241338 2312 30-31 0.3560109280315302 2312 32-33 0.8336897789416362 2312 34-35 0.9612089438926041 2312 36-37 1.1374952703516499 2312 38-39 0.4395224066653505 2312 40-41 0.7923191055519219 2312 42-43 1.6007427494515731 2312 44-45 0.4458939372467583 2312 46-47 -0.13191336004564747 2312 48-49 0.5625201694341726 2312 50-51 0.3660562910771077 2312 52-53 1.0634641696141465 2312 54-55 -0.371173465453289 2312 56-57 -1.3397953671058644 2312 58-59 0.33448366599460755 2312 60-61 -0.5800757409242934 2312 62-63 -0.09717190909599083 2312 64-65 0.11391059604030218 2312 66-67 1.1355674884855809 2312 68-69 1.3821312531341086 2312 70-71 1.6021944997385589 2312 72-73 1.1965149907213757 2312 74-75 1.1823213289956733 2312 76-77 0.6444301374356627 2312 78-79 1.4066479275504271 2312 80-81 1.3442085298977098 2312 82-83 0.7785491743073685 2312 84-85 0.08665531243012836 2312 86-87 -0.03549875226423538 2312 88-89 -0.10488619969616408 2312 90-91 -0.3991718097889354 2312 92-93 1.5728562471817398 2312 94-95 1.5129480335589491 2312 96-97 -0.25541781959278964 2312 98-99 -0.14321798825104537 2312 100 -0.5144040604609366 2313 1 0.36105995283602255 2313 2 0.05727450826489644 2313 3 0.367883493730254 2313 4 -0.027286363942092606 2313 5 0.3556499973563305 2313 6 -0.8274087034605024 2313 7 -0.5566889291533528 2313 8 -0.8818572924503023 2313 9 0.751469447118307 2313 10-11 -0.6518030218432678 2313 12-13 -0.7526683523811037 2313 14-15 -1.4298187395079864 2313 16-17 -0.8139309715336687 2313 18-19 -0.16234499060762886 2313 20-21 -0.7720908627175689 2313 22-23 -0.2357578316606066 2313 24-25 0.3959954004473616 2313 26-27 0.9415792576402282 2313 28-29 0.14188691667077435 2313 30-31 1.525477331193585 2313 32-33 0.1745988698507297 2313 34-35 0.4290943193866781 2313 36-37 0.10291029011449382 2313 38-39 0.5897200351238503 2313 40-41 -0.8628192343690273 2313 42-43 -1.0667473691255012 2313 44-45 0.43205994515189516 2313 46-47 -0.3710437948282568 2313 48-49 -0.09212409934053056 2313 50-51 -0.3286077405434469 2313 52-53 -0.7744804944174746 2313 54-55 0.9554075622146954 2313 56-57 -0.2197360785683138 2313 58-59 -0.14180091898562708 2313 60-61 0.05085311283064797 2313 62-63 0.6997648497972904 2313 64-65 -0.8678087715486278 2313 66-67 -0.021053064874102745 2313 68-69 0.3984356009601946 2313 70-71 -0.08654067812705435 2313 72-73 0.14117902234193025 2313 74-75 0.4056414870984426 2313 76-77 -0.02553033687263806 2313 78-79 0.4837230263646539 2313 80-81 -0.7328665689165206 2313 82-83 0.8699523363627044 2313 84-85 1.0713391068965343 2313 86-87 0.6191455165104678 2313 88-89 -0.3627913380360823 2313 90-91 -0.3818793196703574 2313 92-93 0.5506230456007124 2313 94-95 -0.9208464328442112 2313 96-97 0.8394438404862576 2313 98-99 0.9249638299307712 2313 100 1.9964655047564541 2314 1 0.027572441272653236 2314 2 0.44719125202067644 2314 3 -0.4739296422549444 2314 4 1.1822418506740036 2314 5 0.5184622082721475 2314 6 0.7947004547263639 2314 7 0.8517292022064993 2314 8 1.978919765829069 2314 9 0.5979079300045242 2314 10-11 0.48718865253230703 2314 12-13 1.1714759584422083 2314 14-15 1.828738152258893 2314 16-17 1.4661337833229418 2314 18-19 1.7870074608262314 2314 20-21 0.8153281937116645 2314 22-23 0.35119869007852245 2314 24-25 0.8437289804658619 2314 26-27 0.8927818478344953 2314 28-29 0.9534040304543083 2314 30-31 1.0888445837375258 2314 32-33 1.44055631665924 2314 34-35 1.5257640696179422 2314 36-37 1.8952322142588045 2314 38-39 1.1919402016363385 2314 40-41 1.8039245214126574 2314 42-43 1.7591083200511868 2314 44-45 1.622392970128768 2314 46-47 0.9691504697415887 2314 48-49 0.6599573067649303 2314 50-51 1.4313367552937422 2314 52-53 0.8173326415677238 2314 54-55 1.1401901709103477 2314 56-57 1.320504439470536 2314 58-59 2.1299382114491543 2314 60-61 1.133415555013812 2314 62-63 0.6472671624707402 2314 64-65 0.8854289712820851 2314 66-67 0.7194649739788126 2314 68-69 1.2198972105809176 2314 70-71 0.2719237067018092 2314 72-73 0.8865536754409078 2314 74-75 1.1963445398273933 2314 76-77 1.6555519943022006 2314 78-79 0.4027794555968498 2314 80-81 2.002332321000221 2314 82-83 1.1201836037077548 2314 84-85 2.0907655638808045 2314 86-87 2.398979004022028 2314 88-89 1.9101041291239547 2314 90-91 1.5889809948532339 2314 92-93 2.7423436746478913 2314 94-95 2.849505093520264 2314 96-97 1.5434216388211333 2314 98-99 1.6536872341860906 2314 100 2.639851258688001 2315 1 -2.8716766200540036 2315 2 -2.881344417566307 2315 3 -1.3738044858093872 2315 4 -1.9441661593385113 2315 5 -2.6980584425413703 2315 6 -3.2647488569131866 2315 7 -1.6257426375932518 2315 8 -2.984159082731633 2315 9 -3.5835689160530464 2315 10-11 -4.957429620308741 2315 12-13 -4.009062214273687 2315 14-15 -3.3070565911703937 2315 16-17 -4.51853455209633 2315 18-19 -3.410426832039853 2315 20-21 -4.295748151720126 2315 22-23 -3.8988013099214776 2315 24-25 -3.766408691624253 2315 26-27 -4.3224872385234505 2315 28-29 -3.643279323738433 2315 30-31 -3.9815559168882544 2315 32-33 -4.354812894855154 2315 34-35 -3.6009568315084657 2315 36-37 -3.4814886868676 2315 38-39 -3.1500497858480188 2315 40-41 -3.7464509479240142 2315 42-43 -2.060353507232918 2315 44-45 -4.181111410346826 2315 46-47 -4.087795712986821 2315 48-49 -4.964886247678123 2315 50-51 -4.008275259725032 2315 52-53 -4.782792514877833 2315 54-55 -4.198045123207297 2315 56-57 -5.166667024859873 2315 58-59 -5.075944141492023 2315 60-61 -4.745182692795954 2315 62-63 -3.5323323369034796 2315 64-65 -3.33328191732868 2315 66-67 -4.143175827022439 2315 68-69 -4.169339335101185 2315 70-71 -2.929891061758763 2315 72-73 -3.502682870241191 2315 74-75 -3.093079740523045 2315 76-77 -2.8823078304787764 2315 78-79 -1.9823895056046545 2315 80-81 -3.972636389888386 2315 82-83 -3.4280016278316694 2315 84-85 -2.2656174148425983 2315 86-87 -1.655151158681349 2315 88-89 -3.00729261680846 2315 90-91 -2.8102680664734265 2315 92-93 -3.203213271534839 2315 94-95 -3.5084423407726035 2315 96-97 -2.8383055201275518 2315 98-99 -3.2809185230104028 2315 100 -3.323227589872701 2316 1 1.492702464913318 2316 2 2.022250353675524 2316 3 1.9415549913147956 2316 4 2.8483175007922092 2316 5 1.0356016881775858 2316 6 2.050610620210996 2316 7 3.0556299114263545 2316 8 2.2135533355690207 2316 9 1.4785225872149255 2316 10-11 1.01969456269779 2316 12-13 2.3071142563145486 2316 14-15 2.3195773957577117 2316 16-17 1.9463347289494202 2316 18-19 2.06833133789479 2316 20-21 1.6838270116785665 2316 22-23 1.6567542456340796 2316 24-25 1.8144736613169243 2316 26-27 1.800878774548444 2316 28-29 2.0331912644968604 2316 30-31 2.1287382007588036 2316 32-33 1.8968210920729511 2316 34-35 2.5142392469229122 2316 36-37 1.6198185026748817 2316 38-39 2.1718456389885823 2316 40-41 2.238026176258991 2316 42-43 1.9568033555121822 2316 44-45 1.5582676746204953 2316 46-47 0.9791977510654668 2316 48-49 1.0612575431715499 2316 50-51 1.2498946749154918 2316 52-53 2.6240702302202052 2316 54-55 2.0151901709103477 2316 56-57 3.1021238248133294 2316 58-59 1.96327154478249 2316 60-61 3.2229545621060147 2316 62-63 3.1629291010050196 2316 64-65 1.909365141494849 2316 66-67 2.311072538990633 2316 68-69 1.97935347535633 2316 70-71 2.525174297718358 2316 72-73 2.3821210513274345 2316 74-75 1.9297960764704243 2316 76-77 1.332561450566974 2316 78-79 2.221041866944365 2316 80-81 1.0020368127259935 2316 82-83 1.9085996793578737 2316 84-85 1.6093825851574017 2316 86-87 1.7852083184428338 2316 88-89 1.6160733962634346 2316 90-91 2.3874443518272273 2316 92-93 1.686492610818103 2316 94-95 2.077341972952887 2316 96-97 1.9819559177809474 2316 98-99 1.9666304965974355 2316 100 3.1244848284279527 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 55.0 1 6.0 2 8.0 3 3.0 4 3.0 5 3.0 6 4.0 7 77.0 8 363.0 9 775.0 10 1207.0 11 1460.0 12 1356.0 13 1149.0 14 920.0 15 817.0 16 794.0 17 713.0 18 783.0 19 718.0 20 640.0 21 601.0 22 529.0 23 491.0 24 512.0 25 562.0 26 519.0 27 630.0 28 716.0 29 745.0 30 947.0 31 1151.0 32 1481.0 33 1859.0 34 2613.0 35 3839.0 36 4376.0 37 4544.0 38 3104.0 39 237.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.96611976831544 16.9982793301505 12.342776821850084 24.692824079683977 2 16.004845548152634 21.60872198667474 34.69654754694125 27.68988491823137 3 23.51170974396311 27.28309671156413 26.848683412207258 22.3565101322655 4 16.117758415649348 18.09334271776327 30.381282916292502 35.40761595029488 5 13.841561089267513 35.95699237901073 32.809086937527304 17.392359594194456 6 31.619930137783815 28.96856200271686 18.50135843198137 20.91014942751795 7 25.012128856976517 31.564137395691834 21.618474674946633 21.80525907238502 8 28.88333616360593 29.776085005215787 21.341064020766115 19.99951481041217 9 29.654787608257926 15.125785400645302 18.900560393973944 36.31886659712282 10-11 25.493311341006393 25.77589657138006 24.172558912350066 24.55823317526348 12-13 28.850772701909317 23.420024745869622 24.50205002547369 23.227152526747375 14-15 28.75331950962203 22.63329574253944 23.569427771108444 25.043956976730087 16-17 23.82378619585779 26.423582480477275 27.29786098850463 22.454770335160305 18-19 25.28619936939122 25.47780742178026 28.042687363570217 21.193305845258305 20-21 24.974833537494998 24.427841991000715 26.70798413603231 23.889340335471978 22-23 23.594252106954468 26.155338628509067 23.913175286485174 26.337233978051295 24-25 24.861751152073733 26.95609992723745 22.31991268493815 25.862236235750668 26-27 25.399616756009415 23.753850631867465 24.215926455963324 26.630606156159796 28-29 25.49666464523954 22.82595512431777 26.994542146755606 24.682838083687084 30-31 27.188269396368753 24.39509526870505 25.090053486313085 23.326581848613117 32-33 22.567890453492463 25.460587757280255 27.367220948707686 24.604300840519596 34-35 21.480888630190144 25.954355840124176 27.419237485448196 25.145518044237487 36-37 25.661548909747996 25.414150234058553 24.579786072909844 24.34451478328361 38-39 27.100185551257017 22.731848114774973 24.715912536231006 25.452053797737 40-41 24.34320646241176 25.177692065109287 24.941173616670305 25.53792785580865 42-43 26.78746861696321 24.14462273647952 25.156157139565067 23.9117515069922 44-45 27.65947125879214 22.69585253456221 25.026679602231383 24.61799660441426 46-47 24.432100711920732 25.008186481995804 27.59390198052224 22.965810825561228 48-49 25.529127803316072 24.279840382306208 27.871238492607368 22.319793321770348 50-51 23.21478373691933 24.715953873576737 27.39453612872716 24.674726260776776 52-53 22.240279428529846 25.957503577752444 24.886603439493534 26.915613554224173 54-55 22.994529155597608 26.09022647597559 23.768453485692103 27.146790882734695 56-57 24.01149815641374 23.839268387347175 24.84960217349117 27.299631282747917 58-59 24.417518708534974 22.53150432388507 27.24108236606872 25.809894601511235 60-61 25.314690402852225 24.57010501806893 25.80097499454294 24.31422958453591 62-63 21.50238955872007 24.97513403362364 27.464156619198953 26.05831978845734 64-65 20.512851615094434 25.887604468650306 27.369876639050954 26.229667277204307 66-67 24.29032414596273 24.725834627329192 24.87868788819876 26.105153338509318 68-69 25.089166565570785 23.691617129685795 24.812568239718548 26.406648065024868 70-71 23.756369813152148 24.72822130550837 25.06551807813637 26.449890803203107 72-73 25.659475331861092 23.532409542068095 25.295459509306674 25.51265561676414 74-75 26.046127901192627 23.26171093020152 25.17258532205816 25.51957584654769 76-77 23.857707893523575 24.363640775521098 27.25000606634151 24.528645264613814 78-79 24.85352095590465 24.380420937708497 27.342754897798265 23.423303208588585 80-81 23.57286688177782 24.6658094568039 26.48778475945559 25.273538901962688 82-83 23.025134649910235 24.80833616381193 25.00727837352613 27.15925081275171 84-85 23.75972143021803 25.449824680603246 23.995098336588978 26.79535555258975 86-87 24.630876554443436 23.348498635122837 24.89414619350925 27.126478616924476 88-89 24.928724811044926 22.4817110898128 26.92564329649265 25.663920802649614 90-91 25.73036979520528 23.70790061147239 25.73036979520528 24.831359798117052 92-93 24.080969302979952 24.03366848188621 26.65704479023905 25.228317424894787 94-95 23.415586936477894 23.885087591595088 26.856165380695877 25.843160091231137 96-97 25.680593972921823 23.524773135342357 25.083709419129423 25.710923472606396 98-99 26.196164994966708 22.889958884670897 24.87538052904149 26.038495591320903 100 25.22990318588795 22.91995244219057 26.037900662412344 25.812243709509136 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 21.0 1 25.5 2 24.0 3 12.5 4 6.0 5 4.0 6 3.0 7 9.5 8 14.5 9 15.0 10 16.5 11 13.5 12 9.0 13 11.5 14 16.5 15 20.5 16 20.0 17 19.0 18 23.0 19 27.5 20 25.0 21 20.5 22 25.5 23 31.5 24 34.5 25 49.5 26 65.5 27 80.0 28 98.0 29 115.0 30 143.5 31 177.5 32 194.5 33 201.0 34 257.5 35 321.0 36 360.5 37 424.5 38 470.5 39 509.5 40 545.5 41 572.0 42 589.5 43 617.0 44 692.5 45 750.5 46 928.5 47 1436.5 48 2292.5 49 3497.5 50 5582.0 51 5291.0 52 2644.0 53 1467.0 54 1163.5 55 1040.0 56 920.0 57 795.5 58 762.0 59 747.0 60 661.0 61 576.5 62 538.5 63 463.5 64 399.0 65 375.0 66 340.0 67 279.5 68 242.0 69 206.5 70 160.0 71 142.5 72 135.5 73 121.0 74 100.5 75 76.0 76 54.0 77 52.5 78 36.5 79 23.0 80 25.0 81 20.0 82 16.0 83 10.0 84 3.5 85 4.0 86 3.0 87 0.5 88 1.0 89 1.0 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11377390462357782 2 0.08472524812394094 3 0.2541757443718228 4 0.2590171871217623 5 0.261437908496732 6 0.20818203824739773 7 0.20818203824739773 8 0.21544420237230694 9 0.21544420237230694 10-11 0.20213023480997339 12-13 0.22028564512224644 14-15 0.1851851851851852 16-17 0.1839748244977003 18-19 0.19365770999757928 20-21 0.20455095618494312 22-23 0.1876059065601549 24-25 0.19365770999757928 26-27 0.20091987412248852 28-29 0.205761316872428 30-31 0.20455095618494312 32-33 0.20697167755991283 34-35 0.1888162672476398 36-37 0.19607843137254902 38-39 0.1972887920600339 40-41 0.21060275962236746 42-43 0.20697167755991283 44-45 0.19365770999757928 46-47 0.20213023480997339 48-49 0.2093923989348826 50-51 0.18276446381021544 52-53 0.20091987412248852 54-55 0.2214960058097313 56-57 0.20818203824739773 58-59 0.20697167755991283 60-61 0.19123698862260954 62-63 0.21544420237230694 64-65 0.21665456305979183 66-67 0.22754780924715565 68-69 0.22996853062212538 70-71 0.2420721374969741 72-73 0.2493343016218833 74-75 0.2384410554345195 76-77 0.23965141612200436 78-79 0.22391672718470104 80-81 0.22028564512224644 82-83 0.22270636649721615 84-85 0.24086177680948925 86-87 0.23602033405954975 88-89 0.23359961268458002 90-91 0.23723069474703462 92-93 0.20455095618494312 94-95 0.23238925199709515 96-97 0.23238925199709515 98-99 0.20455095618494312 100 0.23480997337206486 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 41310.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.19704671992254 #Duplication Level Percentage of deduplicated Percentage of total 1 93.84187815402618 62.12055192447349 2 3.795801945439918 5.025417574437182 3 1.111679953192423 2.2076978939724037 4 0.3656841951290865 0.9682885499878964 5 0.17552841366196154 0.5809731299927379 6 0.10239157463614423 0.40668119099491645 7 0.06582315512323558 0.30501089324618735 8 0.06947999707452644 0.3679496489954006 9 0.043882103415490384 0.261437908496732 >10 0.29620419805456005 3.9675623335754056 >50 0.02559789365903606 1.326555313483418 >100 0.09142104878227163 13.657709997579278 >500 0.00731368390258173 2.9774872912127814 >1k 0.00731368390258173 5.826676349552167 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1256 3.040426046961995 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1151 2.786250302590172 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 679 1.643669813604454 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 551 1.3338174776083274 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 423 1.0239651416122004 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 414 1.0021786492374727 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 399 0.9658678286129266 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 399 0.9658678286129266 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 398 0.963447107237957 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 383 0.9271362866134107 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 279 0.6753812636165577 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 217 0.5252965383684338 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 203 0.4914064391188574 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 202 0.4889857177438877 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 201 0.4865649963689179 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 186 0.4502541757443719 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 177 0.42846768336964414 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 175 0.42362624061970466 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 171 0.41394335511982566 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 169 0.40910191236988624 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 168 0.40668119099491645 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 164 0.3969983054950375 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 159 0.3848946986201888 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 151 0.3655289276204309 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 132 0.31953522149600583 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 128 0.3098523359961268 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 121 0.2929072863713386 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 121 0.2929072863713386 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 102 0.24691358024691357 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 97 0.23480997337206486 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 94 0.22754780924715565 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 87 0.21060275962236746 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 77 0.18639554587267004 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 74 0.17913338174776083 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 68 0.1646090534979424 No Hit CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT 51 0.12345679012345678 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 49 0.11861534737351731 No Hit TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA 47 0.11377390462357782 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 46 0.11135318324860807 No Hit ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG 45 0.10893246187363835 No Hit CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC 44 0.10651174049866861 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0012103606874848704 0.0 22-23 0.0 0.0 0.0 0.0036310820624546112 0.0 24-25 0.0 0.0 0.0 0.012103606874848705 0.0 26-27 0.0 0.0 0.0 0.015734688937303315 0.0 28-29 0.0 0.0 0.0 0.0411522633744856 0.0 30-31 0.0 0.0 0.0 0.10409101912369886 0.0 32-33 0.0 0.0 0.0 0.25175502299685304 0.0 34-35 0.0 0.0 0.0 0.51440329218107 0.0 36-37 0.0 0.0 0.0 0.9876543209876543 0.0 38-39 0.0 0.0 0.0 1.572258533042847 0.0 40-41 0.0 0.0 0.0 2.2754780924715563 0.0 42-43 0.0 0.0 0.0 2.868554829339143 0.0 44-45 0.0 0.0 0.0 3.4567901234567904 0.0 46-47 0.0 0.0 0.0 4.185427257322682 0.0 48-49 0.0 0.0 0.0 4.916485112563544 0.0 50-51 0.0 0.0 0.0 5.69837811667877 0.0 52-53 0.0 0.0 0.0 6.483902202856451 0.0 54-55 0.0 0.0 0.0 7.361413701282983 0.0 56-57 0.0 0.0 0.0 8.36480271120794 0.0 58-59 0.0 0.0 0.0 9.267731784071653 0.0 60-61 0.0 0.0 0.0 9.977003146937786 0.0 62-63 0.0 0.0 0.0 10.55918663761801 0.0 64-65 0.0 0.0 0.0 11.301137739046236 0.0 66-67 0.0 0.0 0.0 12.075768579036552 0.0 68-69 0.0 0.0 0.0 13.033163882837085 0.0 70-71 0.0 0.0 0.0 14.368191721132899 0.0 72-73 0.0 0.0 0.0 15.342532074558218 0.0 74-75 0.0 0.0 0.0 16.107480029048656 0.0 76-77 0.0 0.0 0.0 17.05761316872428 0.0 78-79 0.0 0.0 0.0 18.000484144274992 0.0 80-81 0.0 0.0 0.0 18.92641007020092 0.0 82-83 0.0 0.0 0.0 20.158557250060518 0.0 84-85 0.0 0.0 0.0 21.35923505204551 0.0 86-87 0.0 0.0 0.0 22.394093439845072 0.0 88 0.0 0.0 0.0 23.27523602033406 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCTGGG 90 9.340944E-4 26.121237 3 AGTACTT 80 0.0012720135 17.610435 12-13 TACTTTT 110 3.1591393E-5 17.076788 14-15 ACTTTTT 105 4.305022E-4 15.653723 16-17 TGCCGAC 570 1.05878804E-4 9.073693 94 ATGGGAA 1735 0.0 8.139843 5 CATGGGA 1815 0.0 8.040431 4 TGGGAAG 1775 0.0 7.946742 6 TACATGG 2015 0.0 7.9335966 2 CCCATGT 1720 0.0 7.927492 1 GGAAGCA 1800 0.0 7.8363705 8 GGGAAGC 1810 0.0 7.793076 7 ACATGGG 2010 0.0 7.71941 3 GTACATG 2075 0.0 7.704191 1 GAAGCAG 1835 0.0 7.686904 9 GTACTCT 1775 0.0 7.681851 6 TGTACTC 1795 0.0 7.605501 5 ATGTACT 1820 0.0 7.5010295 4 CCATGTA 1820 0.0 7.491915 2 CATGTAC 1915 1.8189894E-12 7.120253 3 >>END_MODULE