##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR522818_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 41310 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.62788671023965 31.0 31.0 34.0 30.0 34.0 2 31.66342290002421 31.0 31.0 34.0 30.0 34.0 3 31.805349794238683 31.0 31.0 34.0 28.0 35.0 4 35.06652142338417 36.0 35.0 37.0 32.0 37.0 5 35.005301379811186 36.0 35.0 37.0 32.0 37.0 6 35.12960542241588 36.0 35.0 37.0 32.0 37.0 7 34.945509561849434 36.0 35.0 37.0 32.0 37.0 8 34.96656983781167 36.0 35.0 37.0 32.0 37.0 9 36.17768094892278 37.0 35.0 39.0 32.0 39.0 10-11 35.977668845315904 37.0 35.0 39.0 31.0 39.0 12-13 35.99950375211813 37.0 35.0 39.0 31.5 39.0 14-15 36.92665214233841 38.0 36.0 40.0 31.0 41.0 16-17 36.67167755991285 37.5 35.5 40.0 31.0 41.0 18-19 36.573614137012825 37.5 35.0 40.0 31.5 41.0 20-21 36.65709271362866 37.5 36.0 40.0 31.5 41.0 22-23 36.52484870491406 37.5 35.5 40.0 30.5 41.0 24-25 36.462309368191725 37.5 35.5 40.0 30.0 41.0 26-27 36.0417695473251 37.0 35.5 40.0 29.5 41.0 28-29 35.93841684822077 37.0 34.5 40.0 29.5 41.0 30-31 35.66410070200919 36.5 34.5 40.0 30.0 41.0 32-33 35.4120309852336 37.0 34.0 40.0 28.0 41.0 34-35 35.257020091987414 37.0 34.0 40.0 27.0 41.0 36-37 35.07482449770032 37.0 34.0 40.0 26.5 41.0 38-39 35.0463931251513 36.5 34.0 40.0 27.0 41.0 40-41 35.27631324134592 37.5 34.0 40.0 27.5 41.0 42-43 35.35382473977245 37.5 34.0 40.0 27.0 41.0 44-45 35.20072621641249 37.0 34.0 40.0 26.5 41.0 46-47 35.041442749939485 37.0 34.0 40.0 26.0 41.0 48-49 34.903231663035584 37.0 33.5 40.0 26.0 41.0 50-51 34.83743645606391 37.0 33.5 40.0 26.0 41.0 52-53 34.850302590171864 37.0 33.0 40.0 26.0 41.0 54-55 34.749939481965626 37.5 33.0 40.0 26.0 41.0 56-57 34.43741224885016 37.0 33.0 39.5 24.0 41.0 58-59 34.23639554587267 36.5 33.0 39.0 24.5 41.0 60-61 33.89926167998063 36.5 32.0 39.0 23.0 40.5 62-63 33.55682643427741 36.0 32.0 39.0 23.0 40.0 64-65 33.447325102880654 36.5 32.0 38.0 23.0 40.0 66-67 32.94483175986444 35.5 31.5 37.0 22.5 40.0 68-69 32.77590171871218 35.5 31.0 37.0 22.0 39.5 70-71 32.3100096828855 35.0 31.0 37.0 22.0 39.0 72-73 32.00718954248366 35.0 30.5 36.0 21.5 39.0 74-75 31.747143548777537 35.0 30.0 36.5 21.0 38.0 76-77 29.995255386105057 32.5 27.5 35.5 19.5 36.0 78-79 31.07084241103849 34.0 29.5 36.0 20.0 37.0 80-81 31.260820624546113 34.0 30.0 36.0 20.0 37.5 82-83 31.20674170902929 34.0 30.0 36.0 20.0 37.0 84-85 30.916182522391672 34.0 30.0 35.5 20.0 36.0 86-87 30.750580973129992 34.0 30.0 35.0 20.0 36.0 88-89 30.680573710965866 34.0 29.5 35.0 19.5 37.0 90-91 30.428576615831517 34.0 29.0 35.0 19.0 36.5 92-93 30.167344468651656 34.0 29.0 35.0 18.0 36.5 94-95 30.281977729363348 34.0 29.0 35.0 18.0 36.5 96-97 30.0920600338901 34.0 29.0 35.0 18.0 36.5 98-99 29.917683369644152 34.0 29.0 35.0 17.0 35.5 100 28.503752118131203 33.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.43316695794869986 1101 2 -0.27442196975943034 1101 3 0.3581868741754839 1101 4 -0.6603982156737658 1101 5 -0.5655130252205254 1101 6 -0.6592856002219065 1101 7 -0.19599354026355087 1101 8 -0.28665699689786805 1101 9 0.41975551631470154 1101 10-11 -0.27465421244698973 1101 12-13 0.5188731014174053 1101 14-15 -0.06911739890482949 1101 16-17 -0.06412288334330896 1101 18-19 -0.3358511778893032 1101 20-21 -0.8172821290796861 1101 22-23 -0.08559459819649362 1101 24-25 0.10268154902870208 1101 26-27 -0.07038366603458712 1101 28-29 -0.21062028267628818 1101 30-31 -0.9538676863039939 1101 32-33 -0.7304026645650694 1101 34-35 0.2711157881450035 1101 36-37 -0.23957799568365346 1101 38-39 -0.09084109420671638 1101 40-41 -0.5339038814257506 1101 42-43 -0.7645653068885494 1101 44-45 -0.11848423450283718 1101 46-47 -0.7106496796387631 1101 48-49 0.2758977905811477 1101 50-51 -0.6790410028129727 1101 52-53 -1.0396866511124188 1101 54-55 -1.065133756698586 1101 56-57 -0.6299889264374556 1101 58-59 -1.2208953192004017 1101 60-61 -0.6931787245600702 1101 62-63 0.18724626436762293 1101 64-65 -0.10202600727537714 1101 66-67 -0.5916921823702097 1101 68-69 0.573823710069334 1101 70-71 -0.7211683687635642 1101 72-73 -0.00318862628218497 1101 74-75 0.511013357247208 1101 76-77 1.438176408914682 1101 78-79 1.2615676078965983 1101 80-81 -0.4713210348300798 1101 82-83 -0.5166984052150561 1101 84-85 -0.2509016324212716 1101 86-87 -0.42433871329200556 1101 88-89 0.5946657877646935 1101 90-91 -0.6749163595612018 1101 92-93 0.3413974221051923 1101 94-95 -0.9877692838069407 1101 96-97 -1.0945195489961357 1101 98-99 -0.13160938451184023 1101 100 -0.19113814904466864 1102 1 0.5375647493683751 1102 2 0.18106583511861984 1102 3 0.18379663027304716 1102 4 -0.01161772786888804 1102 5 0.4735113650233771 1102 6 0.4754704973390673 1102 7 0.6137625572974272 1102 8 -0.4750716310442087 1102 9 1.0404872236317786 1102 10-11 -0.10941031000796642 1102 12-13 0.09112919897838623 1102 14-15 -0.34686130134385706 1102 16-17 -0.018696054075014956 1102 18-19 -0.38036337301125656 1102 20-21 0.0525349440910432 1102 22-23 -0.02187508600137278 1102 24-25 0.5182303295165056 1102 26-27 0.9192504803068786 1102 28-29 0.4424284978115196 1102 30-31 0.24796158198869023 1102 32-33 -0.5166831523699429 1102 34-35 -0.37796957770864736 1102 36-37 -0.8706755566592648 1102 38-39 -0.03138987469451848 1102 40-41 0.8252424600376571 1102 42-43 0.7805566443309502 1102 44-45 1.3525523508630144 1102 46-47 2.194228369141719 1102 48-49 0.7691904735079689 1102 50-51 0.8529711923089778 1102 52-53 1.3673255440095318 1102 54-55 1.1623052676916643 1102 56-57 1.6447061955137698 1102 58-59 2.108068095433744 1102 60-61 1.277248104708221 1102 62-63 0.3518804107090858 1102 64-65 0.32663252930999676 1102 66-67 0.9092224517761238 1102 68-69 0.9665066368986004 1102 70-71 0.5931608995291171 1102 72-73 0.9361406420105034 1102 74-75 0.5771718938325776 1102 76-77 0.6220178723293195 1102 78-79 -0.404895806737553 1102 80-81 0.03569116029187214 1102 82-83 1.3329967167361652 1102 84-85 0.44056178221287823 1102 86-87 0.5805393354884849 1102 88-89 0.8928365194720129 1102 90-91 -0.0718675790733947 1102 92-93 0.7917023001539718 1102 94-95 1.1521697405833038 1102 96-97 1.2484682558819138 1102 98-99 0.4089393959759633 1102 100 1.143008192418744 1103 1 -0.45816695794869844 1103 2 -0.09942196975942963 1103 3 0.20818687417548531 1103 4 0.039601784326237066 1103 5 -0.2905130252205268 1103 6 0.49071439977809206 1103 7 1.004006459736452 1103 8 0.2883430031021277 1103 9 0.04475551631470154 1103 10-11 0.46284578755301453 1103 12-13 0.25637310141741665 1103 14-15 -0.03161739890483517 1103 16-17 -0.5516228833433061 1103 18-19 0.1641488221106968 1103 20-21 -0.0047821290796790095 1103 22-23 0.4769054018035064 1103 24-25 0.5026815490287007 1103 26-27 0.6421163339654186 1103 28-29 0.17687971732371466 1103 30-31 -0.691367686303991 1103 32-33 0.9195973354349363 1103 34-35 -0.02888421185499368 1103 36-37 0.022922004316349387 1103 38-39 -0.5033410942067107 1103 40-41 0.42859611857424085 1103 42-43 -0.23956530688855793 1103 44-45 0.2565157654971628 1103 46-47 -0.0731496796387674 1103 48-49 0.025897790581147717 1103 50-51 0.020958997187030093 1103 52-53 0.5478133488875869 1103 54-55 0.14736624330141979 1103 56-57 0.5450110735625486 1103 58-59 0.5541046807995897 1103 60-61 0.7193212754399312 1103 62-63 0.8622462643676201 1103 64-65 0.2229739927246257 1103 66-67 0.7833078176297832 1103 68-69 1.0363237100693254 1103 70-71 1.32883163123644 1103 72-73 1.2343113737178122 1103 74-75 1.6735133572472165 1103 76-77 -0.5493235910853151 1103 78-79 0.5115676078965983 1103 80-81 0.6286789651699216 1103 82-83 2.2583015947849425 1103 84-85 1.2865983675787298 1103 86-87 0.6631612867079966 1103 88-89 0.0071657877646948975 1103 90-91 -0.09991635956119893 1103 92-93 1.3163974221051902 1103 94-95 1.5872307161930586 1103 96-97 0.767980451003865 1103 98-99 1.7058906154881583 1103 100 0.7338618509553321 1104 1 0.6168330420513009 1104 2 0.3551234847860236 1104 3 0.4809141469027587 1104 4 0.3941472388716889 1104 5 0.37539606568856954 1104 6 0.5475325815962719 1104 7 0.27673373246372535 1104 8 0.5110702758294039 1104 9 0.8697555163147044 1104 10-11 1.1026185148257426 1104 12-13 0.7427367377810512 1104 14-15 0.9183826010951677 1104 16-17 0.2074680257475947 1104 18-19 0.6800579130197875 1104 20-21 1.015672416374862 1104 22-23 1.3223599472580503 1104 24-25 1.3901815490287035 1104 26-27 0.9023436066926891 1104 28-29 1.2121069900509909 1104 30-31 0.8665868591505586 1104 32-33 1.5889155172531204 1104 34-35 1.3404339699631933 1104 36-37 0.8172401861345335 1104 38-39 1.5284770876114706 1104 40-41 1.5660961185742437 1104 42-43 1.5627074203841715 1104 44-45 0.8985612200426161 1104 46-47 0.7643503203612312 1104 48-49 0.807715972399329 1104 50-51 1.7811862699143006 1104 52-53 1.2841769852512215 1104 54-55 0.7485026069377767 1104 56-57 1.1325110735625472 1104 58-59 1.2211501353450487 1104 60-61 1.8102303663490247 1104 62-63 0.7895189916403496 1104 64-65 1.5866103563609855 1104 66-67 2.082171453993425 1104 68-69 2.889732800978422 1104 70-71 2.245877085781892 1104 72-73 1.4411295555360049 1104 74-75 1.4166951754290267 1104 76-77 1.4756764089146834 1104 78-79 2.2661130624420487 1104 80-81 1.6911789651699216 1104 82-83 0.5821652311485792 1104 84-85 -0.025901632421270193 1104 86-87 1.5938431048898103 1104 88-89 1.4787566968556014 1104 90-91 1.5273563677115263 1104 92-93 1.6788974221051909 1104 94-95 2.1020034434657866 1104 96-97 1.3316168146402276 1104 98-99 1.894526979124521 1104 100 2.7361345782280573 1105 1 0.320321414144324 1105 2 0.1447640767521996 1105 3 0.2779543160359488 1105 4 0.07913666804716257 1105 5 0.1507660445469199 1105 6 -0.32033211184981525 1105 7 0.07377390159691544 1105 8 0.10462207286957437 1105 9 -0.26977936740622255 1105 10-11 -0.12756118919117654 1105 12-13 0.3534661246732256 1105 14-15 0.8224523685370286 1105 16-17 0.7518654887497078 1105 18-19 0.8856604500176743 1105 20-21 0.6242876383621763 1105 22-23 0.6315565645942058 1105 24-25 0.0355303862380012 1105 26-27 -0.5418952939415647 1105 28-29 -0.4123644687227923 1105 30-31 0.2397369648588068 1105 32-33 1.0088415214814503 1105 34-35 1.4260576486101186 1105 36-37 0.21100339966518078 1105 38-39 -0.09520155932299446 1105 40-41 -0.05308992793738554 1105 42-43 0.6435742279951668 1105 44-45 1.0312250678227457 1105 46-47 -0.09902177266202727 1105 48-49 0.24508383709277126 1105 50-51 0.4590403925358615 1105 52-53 -0.6080006046007895 1105 54-55 -0.4610639892567221 1105 56-57 0.888325027050918 1105 58-59 0.499162820334476 1105 60-61 0.8966468568352823 1105 62-63 1.297711380646696 1105 64-65 1.13576469039905 1105 66-67 -0.507680554463235 1105 68-69 1.1156841751856064 1105 70-71 0.6779595382131802 1105 72-73 0.6139625365085202 1105 74-75 0.7002575432937235 1105 76-77 -0.09409103294578003 1105 78-79 -0.028548671173172835 1105 80-81 0.5603650116815508 1105 82-83 0.4719643854826181 1105 84-85 0.901423948974081 1105 86-87 0.8570566355452094 1105 88-89 -0.45940397967716606 1105 90-91 0.42217666369461426 1105 92-93 0.5131997476865848 1105 94-95 0.7613586231698051 1105 96-97 0.3633292882131691 1105 98-99 0.06577433641839292 1105 100 0.043745571885562384 1106 1 0.41445208967035185 1106 2 0.019625649288190772 1106 3 0.3986630646516751 1106 4 0.13007797480242544 1106 5 -0.06075112045861886 1106 6 0.46095249501618696 1106 7 0.504006459736452 1106 8 -0.2735617588026287 1106 9 -0.2731016265424415 1106 10-11 -0.39251135530412995 1106 12-13 0.26708738713170277 1106 14-15 -0.26614120842864253 1106 16-17 0.786472354751929 1106 18-19 0.04152977449164297 1106 20-21 -0.3333535576511153 1106 22-23 0.5269054018035035 1106 24-25 -0.2883898795427271 1106 26-27 0.718902048251131 1106 28-29 0.4453320982760971 1106 30-31 0.5473227898864934 1106 32-33 -0.18218837885077477 1106 34-35 0.24194912147833492 1106 36-37 0.9568505757449159 1106 38-39 0.918087477221853 1106 40-41 0.7863342138123457 1106 42-43 0.9782918359685837 1106 44-45 0.503539575020973 1106 46-47 0.4964931775040924 1106 48-49 1.2270882667716165 1106 50-51 1.0025066162346477 1106 52-53 1.433527634601873 1106 54-55 0.7836757671109424 1106 56-57 0.7158444068958829 1106 58-59 0.7785094427043546 1106 60-61 0.2014641325827924 1106 62-63 0.008674835796192326 1106 64-65 -0.1698831501325202 1106 66-67 0.3386649604869234 1106 68-69 1.114299900545518 1106 70-71 0.2734744883792999 1106 72-73 0.6997875641940077 1106 74-75 -1.1217247379908812 1106 76-77 0.035200218438493636 1106 78-79 0.7103771317061209 1106 80-81 -0.2790591300681733 1106 82-83 -0.5006269766436269 1106 84-85 0.6705269390072992 1106 86-87 1.8589946200413259 1106 88-89 0.6410943591932643 1106 90-91 0.6096074499626098 1106 92-93 0.3158021840099501 1106 94-95 -0.012174045711702774 1106 96-97 1.348932831956244 1106 98-99 1.376128710726256 1106 100 1.246957089050568 1107 1 0.2077421329603908 1107 2 0.7414871211496639 1107 3 0.43545960144821194 1107 4 -0.1967618520373975 1107 5 -0.010967570675070704 1107 6 0.6384416725053654 1107 7 0.09491555064554547 1107 8 0.6928884576475838 1107 9 1.097028243587431 1107 10-11 0.7958003330075556 1107 12-13 0.2768276468719577 1107 14-15 0.3615644192769878 1107 16-17 0.9233771166566882 1107 18-19 0.4755124584743342 1107 20-21 1.0611269618294088 1107 22-23 0.6973599472580503 1107 24-25 0.731090639937797 1107 26-27 0.03870724305632223 1107 28-29 0.13256153550553051 1107 30-31 1.1393141318778248 1107 32-33 0.88437006270766 1107 34-35 1.260888515417733 1107 36-37 0.6240583679527063 1107 38-39 1.3921134512478304 1107 40-41 0.7138233913015171 1107 42-43 0.8467983294750852 1107 44-45 0.19401576549716282 1107 46-47 0.7302594112703247 1107 48-49 0.5349886996720485 1107 50-51 0.928913542641574 1107 52-53 -0.238550287476059 1107 54-55 1.4644116978468702 1107 56-57 0.2575110735625472 1107 58-59 0.8916046807995954 1107 60-61 0.7193212754399312 1107 62-63 0.6872462643676229 1107 64-65 0.00706490181553221 1107 66-67 0.6958078176297846 1107 68-69 0.9351873464329685 1107 70-71 -0.3223047323999211 1107 72-73 0.7820386464450841 1107 74-75 0.06442244815630715 1107 76-77 0.5665854998237769 1107 78-79 1.2092948806238688 1107 80-81 1.1116335106244684 1107 82-83 1.7412561402394893 1107 84-85 1.7127347312150896 1107 86-87 1.7756612867079973 1107 88-89 1.8992112423101482 1107 90-91 1.504629094984253 1107 92-93 0.9175337857415542 1107 94-95 1.3179125343748765 1107 96-97 2.2861622691856773 1107 98-99 2.3604360700336144 1107 100 3.190680032773514 1108 1 -0.20816695794869844 1108 2 0.20057803024057108 1108 3 -0.16681312582451469 1108 4 -0.1353982156737672 1108 5 0.2094869747794732 1108 6 0.09071439977809348 1108 7 -0.6459935402635466 1108 8 0.23834300310213052 1108 9 0.4697555163147058 1108 10-11 -0.06215421244699115 1108 12-13 0.35637310141741096 1108 14-15 -0.14411739890483233 1108 16-17 0.5233771166566896 1108 18-19 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0.5904903767359926 2316 70-71 0.4718871867919958 2316 72-73 0.6204224848289321 2316 74-75 -0.6514866427527863 2316 76-77 -0.6076569244186487 2316 78-79 -0.5217657254367403 2316 80-81 -0.2671543681634141 2316 82-83 0.2096904836738318 2316 84-85 -0.8453460768657131 2316 86-87 -0.5437831577364456 2316 88-89 -0.24977865667974797 2316 90-91 0.8556391959943568 2316 92-93 0.5400085332163016 2316 94-95 -0.1947137282513829 2316 96-97 -0.4209084378850214 2316 98-99 -0.5802204956229495 2316 100 0.1755285176219985 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 12.0 15 36.0 16 80.0 17 118.0 18 194.0 19 293.0 20 299.0 21 320.0 22 376.0 23 454.0 24 528.0 25 628.0 26 773.0 27 863.0 28 1036.0 29 1318.0 30 1516.0 31 1781.0 32 2268.0 33 2800.0 34 3655.0 35 5514.0 36 6876.0 37 5520.0 38 3775.0 39 276.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.14985227878142 16.229960769119 10.626241100401995 24.993945851697582 2 15.48852522513799 21.729447080468674 34.61557083373681 28.16645686065653 3 21.830065359477125 28.295812152021306 29.17937545388526 20.694747034616316 4 14.161220043572984 17.91817961752602 31.428225611232147 36.492374727668846 5 11.825223916727184 34.739772452190756 35.904139433551194 17.530864197530864 6 30.36552892762043 28.424110384894696 20.784313725490197 20.426046961994672 7 22.79593318809005 31.03606874848705 24.340353425320746 21.827644638102154 8 27.59380295328008 29.81360445412733 23.708545146453645 18.88404744613895 9 27.143548777535702 15.957395303800531 21.089324618736384 35.80973129992738 10-11 24.14548535463568 25.098039215686274 26.042120551924477 24.714354877753568 12-13 27.349641123712466 23.15690095498614 26.420072864595312 23.073385056706083 14-15 27.325109854858432 22.438233122299025 25.954799113898 24.281857908944545 16-17 23.209876543209877 25.81941418542726 28.86468167513919 22.106027596223672 18-19 23.867102396514163 25.69232631324135 29.717985959816023 20.722585330428466 20-21 23.034374243524567 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26.279095101492427 24.70193785782587 46-47 22.564400542320357 25.393424365678868 29.247772612822004 22.79440247917877 48-49 23.855410018642715 24.14110355180011 29.813814977120305 22.18967145243687 50-51 21.427447372562312 24.699487949254923 28.909682964325924 24.963381713856844 52-53 20.71006633418874 25.664552365273813 26.421101050694812 27.20428024984264 54-55 21.48252154546335 25.602788806042415 25.88360608114651 27.03108356734773 56-57 23.154110583906267 23.294519221458312 26.405296794809725 27.1460733998257 58-59 23.05206202413664 22.5606139469575 28.71097769116241 25.676346337743457 60-61 23.89580836873767 25.089267342072453 26.89398065773388 24.120943631455997 62-63 20.038731541999518 25.791575889615103 27.685790365528927 26.483902202856452 64-65 19.186637618010167 26.49842653110627 28.526991043330913 25.78794480755265 66-67 22.862503025901717 25.325587024933434 25.813362381989833 25.998547567175017 68-69 23.70491406439119 24.051077221011862 25.031469377874604 27.21253933672234 70-71 22.200435729847495 24.99515855725006 25.23602033405955 27.568385378842898 72-73 24.22687275324078 24.17119548772074 25.50260835885208 26.099323400186396 74-75 24.88440775617904 23.78900481735215 25.01997143479629 26.306615991672516 76-77 22.15497833401922 24.63748819869762 27.446803360042605 25.760730107240555 78-79 23.488543797431582 25.013616722545663 26.835231605320807 24.662607874701944 80-81 21.753812636165577 25.360687484870493 25.950133139675625 26.93536673928831 82-83 20.930767368675866 25.491406439118858 24.87654320987654 28.701282982328735 84-85 22.473977245219075 25.351004599370615 24.026870007262165 28.14814814814815 86-87 22.931493585088354 23.74243524570322 25.226337448559672 28.09973372064875 88-89 22.42072137496974 23.114258048898574 26.776809489227787 27.688211086903898 90-91 23.431372549019606 24.014766400387316 25.781893004115226 26.771968046477852 92-93 21.226095376422176 24.73372064875333 26.5117404986686 27.528443476155896 94-95 20.400629387557494 24.403292181069958 26.99346405228758 28.202614379084967 96-97 21.83127572016461 23.858629871701766 26.56499636891794 27.745098039215687 98-99 23.125151295085935 22.450980392156865 25.94650205761317 28.477366255144034 100 21.534737351730815 23.902202856451222 26.899055918663763 27.664003873154204 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4.0 1 3.0 2 10.5 3 15.5 4 10.5 5 8.5 6 12.0 7 18.0 8 15.5 9 22.0 10 30.5 11 23.5 12 24.5 13 28.5 14 24.0 15 23.5 16 28.5 17 30.0 18 32.5 19 38.5 20 48.0 21 50.5 22 46.5 23 55.0 24 62.0 25 65.0 26 77.0 27 98.0 28 109.5 29 118.0 30 137.5 31 174.0 32 210.0 33 274.5 34 349.5 35 391.5 36 479.5 37 517.0 38 491.5 39 529.5 40 573.0 41 566.5 42 638.5 43 723.0 44 732.0 45 804.5 46 987.5 47 1389.5 48 2050.5 49 2939.0 50 4868.0 51 5137.5 52 3400.5 53 2653.0 54 2009.5 55 1277.0 56 929.5 57 815.0 58 743.0 59 633.0 60 525.5 61 435.5 62 370.5 63 297.5 64 210.0 65 160.0 66 130.0 67 108.5 68 100.0 69 86.5 70 69.0 71 55.5 72 51.5 73 55.0 74 47.5 75 26.0 76 12.5 77 4.5 78 3.5 79 3.0 80 1.0 81 1.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03873154199951585 2 0.004841442749939482 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0012103606874848704 14-15 0.013313967562333577 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.002420721374969741 24-25 0.002420721374969741 26-27 0.019365770999757927 28-29 0.013313967562333577 30-31 0.009682885499878963 32-33 0.010893246187363833 34-35 0.007262164124909223 36-37 0.002420721374969741 38-39 0.002420721374969741 40-41 0.0036310820624546117 42-43 0.007262164124909223 44-45 0.0036310820624546117 46-47 0.014524328249818447 48-49 0.016945049624788187 50-51 0.013313967562333577 52-53 0.009682885499878963 54-55 0.004841442749939482 56-57 0.004841442749939482 58-59 0.008472524812394093 60-61 0.0036310820624546117 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0012103606874848704 74-75 0.002420721374969741 76-77 0.002420721374969741 78-79 0.0012103606874848704 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 41310.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.67586540789155 #Duplication Level Percentage of deduplicated Percentage of total 1 89.55402450596146 52.54659888646817 2 6.312141589999587 7.4074074074074066 3 1.8358843186600107 3.231663035584604 4 0.7013490655555097 1.646090534979424 5 0.301167539915013 0.8835633018639554 6 0.2269070506209002 0.7988380537400145 7 0.1320186476339783 0.542241587993222 8 0.12376748215685465 0.5809731299927379 9 0.11139073394116918 0.5882352941176471 >10 0.5239490077973513 6.315662067296055 >50 0.04538141012418004 1.905107722101186 >100 0.11551631667973102 14.601791333817477 >500 0.008251165477123644 2.9605422415879934 >1k 0.008251165477123644 5.991285403050109 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1288 3.1178891309610264 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1187 2.8733962720890824 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 620 1.5008472524812393 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 603 1.4596949891067539 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 469 1.1353183248608085 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 401 0.9707092713628662 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 395 0.9561849431130478 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 393 0.9513435003631082 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 386 0.9343984507383201 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 376 0.9101912369886226 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 234 0.5664488017429194 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 230 0.5567659162430404 No Hit CCACTGTACCGTATGCGAATCTTTGCACCCAACCATGTGGTGGCCAAGTC 223 0.5398208666182522 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 218 0.5277172597434036 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 197 0.476882110869039 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 185 0.4478334543694021 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 181 0.4381505688695231 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 180 0.4357298474945534 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 180 0.4357298474945534 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 170 0.411522633744856 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 168 0.40668119099491645 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 166 0.40183974824497704 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 155 0.3752118131203098 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 155 0.3752118131203098 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 146 0.3534253207455822 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 146 0.3534253207455822 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 127 0.3074316146211571 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 122 0.2953280077463084 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 112 0.271120793996611 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 107 0.2590171871217623 No Hit CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 106 0.25659646574679257 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 104 0.25175502299685304 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 99 0.23965141612200436 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 95 0.22996853062212538 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 87 0.21060275962236746 No Hit ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT 86 0.20818203824739773 TruSeq Adapter, Index 10 (95% over 21bp) GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 80 0.19365770999757928 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 70 0.1694504962478819 No Hit GTACTTTGTGTCACAGCTGAAGAAGATGAAGAAGTCATCCGGGGAAATTG 57 0.13798111837327523 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 55 0.13313967562333576 No Hit GTCCCGCTTCTGGTACTTTGTGTCACAGCTGAAGAAGATGAAGAAGTCAT 54 0.130718954248366 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 53 0.12829823287339628 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 51 0.12345679012345678 No Hit ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG 50 0.12103606874848705 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 50 0.12103606874848705 No Hit CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT 49 0.11861534737351731 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 46 0.11135318324860807 No Hit GTATAGGAAGACGCTGGGTTGGTCCGATACTATTTACACGTGGTGGCAGC 45 0.10893246187363835 No Hit GGTCACAAGCCTCGTGCTCTGCATTGTGAGCCATGTTGTAGGGGACGATT 45 0.10893246187363835 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 45 0.10893246187363835 No Hit AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA 45 0.10893246187363835 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 43 0.10409101912369886 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0012103606874848704 0.0 22-23 0.0 0.0 0.0 0.0036310820624546112 0.0 24-25 0.0 0.0 0.0 0.012103606874848705 0.0 26-27 0.0 0.0 0.0 0.015734688937303315 0.0 28-29 0.0 0.0 0.0 0.03994190268700072 0.0 30-31 0.0 0.0 0.0 0.10045993706124424 0.0 32-33 0.0 0.0 0.0 0.24449285887194383 0.0 34-35 0.0 0.0 0.0 0.5035100459937061 0.0 36-37 0.0 0.0 0.0 0.9682885499878964 0.0 38-39 0.0 0.0 0.0 1.5541031227305737 0.0 40-41 0.0 0.0 0.0 2.2452190752844348 0.0 42-43 0.0 0.0 0.0 2.822561123214718 0.0 44-45 0.0 0.0 0.0 3.4095860566448803 0.0 46-47 0.0 0.0 0.0 4.127329944323408 0.0 48-49 0.0 0.0 0.0 4.846284192689421 0.0 50-51 0.0 0.0 0.0 5.619704671992253 0.0 52-53 0.0 0.0 0.0 6.393125151295086 0.0 54-55 0.0 0.0 0.0 7.260953764221738 0.0 56-57 0.0 0.0 0.0 8.243766642459452 0.0 58-59 0.0 0.0 0.0 9.123698862260953 0.0 60-61 0.0 0.0 0.0 9.82328733962721 0.0 62-63 0.0 0.0 0.0 10.387315419995158 0.0 64-65 0.0 0.0 0.0 11.114742193173566 0.0 66-67 0.0 0.0 0.0 11.87121762285161 0.0 68-69 0.0 0.0 0.0 12.827402565964658 0.0 70-71 0.0 0.0 0.0 14.134592108448317 0.0 72-73 0.0 0.0 0.0 15.077463083999032 0.0 74-75 0.0 0.0 0.0 15.82788671023965 0.0 76-77 0.0 0.0 0.0 16.768336964415397 0.0 78-79 0.0 0.0 0.0 17.684580004841443 0.0 80-81 0.0 0.0 0.0 18.598402323892522 0.0 82-83 0.0 0.0 0.0 19.81844589687727 0.0 84-85 0.0 0.0 0.0 20.99128540305011 0.0 86-87 0.0 0.0 0.0 21.96562575647543 0.0 88 0.0 0.0 0.0 22.82982328733963 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTATG 35 3.5623034E-6 67.18874 9 ACCGTAT 35 3.5623034E-6 67.18874 8 GTACCGT 35 3.588093E-6 67.10741 6 TACCGTA 40 7.922958E-6 58.718975 7 TACCCAT 25 0.0052113542 56.37022 2 AAGATGC 25 0.0052113542 56.37022 6 ACCCATG 25 0.0052113542 56.37022 3 CACTGTA 45 1.5917503E-5 52.194645 2 ACTGTAC 45 1.5917503E-5 52.194645 3 TGTACCG 45 1.5917503E-5 52.194645 5 TATGCGA 40 2.4087603E-7 41.153107 12-13 TACTTTT 105 1.0895747E-9 40.264442 2 TCATCCG 60 8.704205E-5 39.145985 94 TGCGAAT 45 6.0943603E-7 36.55838 14-15 CCACTGT 65 1.394367E-4 36.134758 1 TGGTACT 55 7.585004E-8 34.16377 58-59 TGCACCC 50 1.3697718E-6 32.96244 24-25 AAGTCCC 50 1.3697718E-6 32.96244 46-47 TTTGCAC 50 1.3762929E-6 32.94245 22-23 CCCGCTT 50 1.3828394E-6 32.922485 50-51 >>END_MODULE