FastQCFastQC Report
Mon 6 Jun 2016
ERR1211655_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211655_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7368090
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255190.3463448464934603No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213340.2895458660249807No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147580.20029614187666No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106130.14404004294192932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118800.046.6497541
TACCTGG87750.046.2177932
GTACCTG91400.045.308141
TATAACG8900.039.119272
GTATCAA203250.038.6841431
ACCTGGG104200.038.3725663
TAACGCA10000.033.829644
TCAACGC244750.031.4645214
ATAACGC11400.031.3233183
ATCAACG247200.031.1142393
CAACGCA250500.030.8706465
AACGCAG260450.029.7229296
TATCAAC259950.029.610282
GTACATG228750.028.9284041
TACATGG232700.027.3760812
ACGCAGA292500.026.273757
ACATGGG237200.025.9090823
CCTGGGG101250.025.7086724
CGCAGAG298800.025.6418768
CATGGGG151800.024.761854
Produced by FastQC (version 0.11.5)