FastQCFastQC Report
Mon 6 Jun 2016
ERR1211655_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211655_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7368090
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298820.4055596497871226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254180.3449740706207443No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181530.24637321205359866No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112390.15253613894509976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG89150.047.680622
GGTATCA126950.047.6084561
GTACCTG93000.046.5866281
ACCTGGG105250.039.288593
GTATCAA220300.038.7982441
TAACGCA10200.036.8549354
TATAACG10250.034.4058382
ATAACGC12350.031.1998673
TCAACGC276000.030.5605554
ATCAACG278550.030.2133083
CAACGCA281600.029.9522065
TATCAAC289650.029.1722092
CCTGGGG91750.029.039084
AACGCAG293300.028.9185756
GTACATG238700.027.8480911
TACATGG243700.026.781122
ACATGGG238900.026.120883
CATGGGG147400.025.5352974
ACGCAGA331200.025.481497
CGCAGAG340850.024.6497788
Produced by FastQC (version 0.11.5)