FastQCFastQC Report
Mon 6 Jun 2016
ERR1211654_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211654_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10080542
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291160.2888336758083047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251600.24958975420170862No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166810.16547721342761135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144700.051.0742451
GTATCAA252250.038.96441
TCAACGC305900.031.2613774
CAACGCA308550.030.9269055
ATCAACG311800.030.6237143
GTACATG338550.029.923541
AACGCAG322300.029.704686
TATCAAC333950.028.8551832
TACATGG347050.028.7556952
ACATGGG354600.027.391263
ACGCAGA365900.025.9342847
CATGGGG231650.025.3977494
CGCAGAG379700.024.8439888
GCAGAGT422050.021.6498039
GTACCTG82250.020.2413861
GAGTACA242500.020.1877121
ATGGGGG136800.019.9889035
AGAGTAC387200.019.68109510-11
GAGTACT271850.019.39970412-13
CAGAGTA414300.019.36330810-11
Produced by FastQC (version 0.11.5)