FastQCFastQC Report
Mon 6 Jun 2016
ERR1211654_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211654_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10080542
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346900.3441283216715927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT305780.3033368642281338No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211900.21020695117385554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA152800.051.7737351
GTATCAA274850.039.7439651
TCAACGC340850.031.1978844
ATCAACG345800.030.7929763
CAACGCA347050.030.6133025
AACGCAG360200.029.6139816
GTACATG351650.029.5221251
TATCAAC365650.029.4160062
TACATGG364350.028.075252
ACATGGG369600.026.6359883
ACGCAGA412550.025.6283457
CGCAGAG427250.024.6585858
CATGGGG241100.024.6350654
GTACCTG82450.023.3869481
TACCTGG83950.021.7927512
GCAGAGT479900.021.590889
ATGGGGG137200.021.1316175
AGAGTAC437850.020.42866510-11
GAGTACT302050.019.7728612-13
AGTACTT321200.018.8792712-13
Produced by FastQC (version 0.11.5)