##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211654_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10080542 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.994245150707176 33.0 31.0 34.0 30.0 34.0 2 32.263041213458564 34.0 31.0 34.0 30.0 34.0 3 32.35532166821982 34.0 31.0 34.0 30.0 34.0 4 35.99821428252568 37.0 35.0 37.0 35.0 37.0 5 35.89173895609978 37.0 35.0 37.0 35.0 37.0 6 35.92192850344753 37.0 35.0 37.0 35.0 37.0 7 35.82349837935301 37.0 35.0 37.0 35.0 37.0 8 35.80699857210059 37.0 35.0 37.0 35.0 37.0 9 37.44411024724663 39.0 37.0 39.0 34.0 39.0 10-11 37.4023448342361 39.0 37.0 39.0 34.0 39.0 12-13 37.30569412835143 39.0 37.0 39.0 34.0 39.0 14-15 38.51412691896924 40.0 38.0 41.0 34.0 41.0 16-17 38.443551199925565 40.0 38.0 41.0 34.0 41.0 18-19 38.43356795696104 40.0 38.0 41.0 34.0 41.0 20-21 38.36621393968697 40.0 38.0 41.0 34.0 41.0 22-23 38.259563523469275 40.0 38.0 41.0 34.0 41.0 24-25 38.14397058213735 40.0 37.5 41.0 33.0 41.0 26-27 37.933859607945685 40.0 37.0 41.0 33.0 41.0 28-29 37.95493655003868 40.0 37.0 41.0 33.0 41.0 30-31 37.84335792658768 40.0 37.0 41.0 33.0 41.0 32-33 37.66656381174742 40.0 37.0 41.0 32.5 41.0 34-35 37.53136994022742 40.0 37.0 41.0 32.0 41.0 36-37 37.43502606308272 40.0 36.5 41.0 32.0 41.0 38-39 37.356404050496494 40.0 36.0 41.0 31.0 41.0 40-41 37.19728190210407 39.5 36.0 41.0 31.0 41.0 42-43 37.00695438796842 39.0 36.0 41.0 30.5 41.0 44-45 36.79941763052027 39.0 35.5 41.0 30.0 41.0 46-47 36.543112017191135 39.0 35.0 41.0 30.0 41.0 48-49 36.28376981118674 39.0 35.0 40.0 29.0 41.0 50-51 36.06615313938477 39.0 35.0 40.0 28.0 41.0 52-53 35.8498307928284 38.5 35.0 40.0 27.5 41.0 54-55 35.62165223854035 38.0 34.0 40.0 27.0 41.0 56-57 35.396344760033735 38.0 34.0 40.0 26.5 41.0 58-59 35.1141488721539 38.0 34.0 40.0 26.0 41.0 60-61 34.785429741773804 37.5 33.0 40.0 26.0 41.0 62-63 34.43531205960949 37.0 33.0 40.0 25.0 41.0 64-65 34.09625340581886 36.0 32.5 39.0 25.0 41.0 66-67 33.722670368319484 36.0 32.0 39.0 23.5 40.0 68-69 33.31269995204623 35.0 31.5 39.0 23.0 40.0 70-71 32.93018500394126 35.0 31.0 38.0 22.5 40.0 72-73 32.5048217149435 35.0 31.0 37.5 22.0 39.5 74-75 32.1047132683937 35.0 30.5 37.0 21.0 39.0 76-77 30.650303326944126 33.5 28.5 35.5 20.0 37.5 78-79 31.511484699929824 34.0 30.0 36.0 21.0 38.0 80-81 31.47403775511277 34.0 30.0 35.5 20.0 37.0 82-83 31.19531901161664 34.0 30.0 35.0 20.0 37.0 84-85 30.877383378790544 34.0 30.0 35.0 20.0 36.5 86-87 30.57726697631933 34.0 29.0 35.0 19.0 36.0 88-89 30.291663335165907 34.0 29.0 35.0 18.5 36.0 90-91 30.00551746126349 34.0 29.0 35.0 18.0 35.0 92-93 29.765399271189985 34.0 29.0 35.0 18.0 35.0 94-95 29.539508639515613 33.5 28.5 35.0 17.0 35.0 96-97 29.297374238409006 33.0 27.0 35.0 15.5 35.0 98-99 29.08803375850227 33.0 27.0 35.0 15.0 35.0 100 27.271756022642432 31.0 24.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25798930441384016 1101 2 0.16879131446778928 1101 3 0.16542869326777065 1101 4 0.12447305898556493 1101 5 0.11212491163425398 1101 6 0.10484362305883366 1101 7 0.12751421586276734 1101 8 0.10941678625582085 1101 9 0.13812962436449538 1101 10-11 0.1258668564336034 1101 12-13 0.08615268601382553 1101 14-15 0.15175762213778654 1101 16-17 0.1913620676994796 1101 18-19 0.10233994406299018 1101 20-21 0.09410200725670848 1101 22-23 -0.007726519296319623 1101 24-25 -0.020306749846284333 1101 26-27 -0.07549361402021759 1101 28-29 0.06304565417514851 1101 30-31 0.11918603706023134 1101 32-33 0.10258823953433449 1101 34-35 0.09247390950765322 1101 36-37 0.042189587467483136 1101 38-39 -0.051581474929299986 1101 40-41 -0.05315405942323537 1101 42-43 -0.013845568153413979 1101 44-45 -0.03857717823475326 1101 46-47 -0.14588825504922198 1101 48-49 -0.10564726434485294 1101 50-51 -0.16066416782555137 1101 52-53 -0.21375573356962008 1101 54-55 -0.3049232987528967 1101 56-57 -0.38194416875486326 1101 58-59 -0.4085160831709871 1101 60-61 -0.3426716427354677 1101 62-63 -0.5069038590069468 1101 64-65 -0.4906447225726822 1101 66-67 -0.4869224662705136 1101 68-69 -0.46856212723226065 1101 70-71 -0.5010649038756512 1101 72-73 -0.6420171243533943 1101 74-75 -0.7773043276788236 1101 76-77 -0.6303528058554022 1101 78-79 -0.5898964898078916 1101 80-81 -0.5245166017264147 1101 82-83 -0.6106798215824796 1101 84-85 -0.6953583576778328 1101 86-87 -0.7092908735206329 1101 88-89 -0.6931285345788218 1101 90-91 -0.7705392038417749 1101 92-93 -0.9419662449132673 1101 94-95 -1.0019002821546223 1101 96-97 -1.159473535895465 1101 98-99 -1.265275610803151 1101 100 -1.2074112151618905 1102 1 0.22150046433081627 1102 2 0.16110722763418295 1102 3 0.12741762076270646 1102 4 0.089234872214206 1102 5 0.08868949264086012 1102 6 0.09675686533920214 1102 7 0.10045050716739468 1102 8 0.07065984871496767 1102 9 0.06636231049154162 1102 10-11 0.11351481857621337 1102 12-13 0.15054100299101947 1102 14-15 0.1538946135842849 1102 16-17 0.169021629941021 1102 18-19 0.05543571862477137 1102 20-21 0.08239237888988526 1102 22-23 0.06494645529564735 1102 24-25 0.058390780618317706 1102 26-27 0.0019553510347449787 1102 28-29 0.07582584955893168 1102 30-31 0.0626742497951227 1102 32-33 0.11075740295063241 1102 34-35 0.08044421050595929 1102 36-37 0.00230109244443355 1102 38-39 0.02493786124048114 1102 40-41 -0.048149581300464206 1102 42-43 -0.04695224896631345 1102 44-45 -0.07698085698904578 1102 46-47 -0.08851685954039823 1102 48-49 -0.10074794530555664 1102 50-51 -0.07939143798694914 1102 52-53 -0.15844269152474766 1102 54-55 -0.15160944567197276 1102 56-57 -0.17361344253100697 1102 58-59 -0.1847312139141266 1102 60-61 -0.16277064459588075 1102 62-63 -0.23762964535331577 1102 64-65 -0.20383299555967938 1102 66-67 -0.37947515606771276 1102 68-69 -0.3213866396651426 1102 70-71 -0.2695304635853901 1102 72-73 -0.4297884274099104 1102 74-75 -0.5113718012126967 1102 76-77 -0.3660754067750709 1102 78-79 -0.2993878760209938 1102 80-81 -0.359065162052012 1102 82-83 -0.4468772313521079 1102 84-85 -0.5044768597744032 1102 86-87 -0.48446151704117213 1102 88-89 -0.518570425518039 1102 90-91 -0.6572197024164517 1102 92-93 -0.6889960405753293 1102 94-95 -0.6567063113589242 1102 96-97 -0.7256159468143437 1102 98-99 -0.7766975784687808 1102 100 -0.720823475430759 1103 1 0.24102586346211297 1103 2 0.17947064643976773 1103 3 0.15382594812232497 1103 4 0.13405403159718077 1103 5 0.11505545393568894 1103 6 0.13250073707292387 1103 7 0.11246942952555372 1103 8 0.12589609731513463 1103 9 0.1469360269376807 1103 10-11 0.11313980948102653 1103 12-13 0.15012696758282118 1103 14-15 0.18339291494525156 1103 16-17 0.15345708730520613 1103 18-19 0.12425495350864679 1103 20-21 0.12226514541680444 1103 22-23 0.11343405526633177 1103 24-25 0.014361017538433885 1103 26-27 0.008761680742473743 1103 28-29 0.0838168530181278 1103 30-31 0.13178948894368148 1103 32-33 0.15918789879457051 1103 34-35 0.0820307567774492 1103 36-37 0.061935653426751 1103 38-39 0.11919408575915469 1103 40-41 0.02719035008151849 1103 42-43 0.03131036862531289 1103 44-45 0.03645549167608664 1103 46-47 0.07668756028711954 1103 48-49 0.12653619349647727 1103 50-51 0.03570785846244462 1103 52-53 0.01021102582350153 1103 54-55 0.01959664883467127 1103 56-57 0.006009572291453935 1103 58-59 -0.11328492652293676 1103 60-61 -0.13769971896265076 1103 62-63 -0.07014347145664601 1103 64-65 -0.21477584238736824 1103 66-67 -0.19052735725120584 1103 68-69 -0.23422268295688298 1103 70-71 -0.22136604088793632 1103 72-73 -0.2370133809320123 1103 74-75 -0.2230793517573062 1103 76-77 -0.1630741622859908 1103 78-79 -0.13055050984981875 1103 80-81 -0.13081932483684255 1103 82-83 -0.09069497835780282 1103 84-85 -0.17492342811752692 1103 86-87 -0.17105670463897127 1103 88-89 -0.23191535504495775 1103 90-91 -0.22414578117598083 1103 92-93 -0.33852545266231004 1103 94-95 -0.23708753239244018 1103 96-97 -0.38464536728822907 1103 98-99 -0.4033964567287107 1103 100 -0.4514875040187327 1104 1 0.2590156495506051 1104 2 0.12008351968293596 1104 3 0.12729855859667794 1104 4 0.10073683939779698 1104 5 0.07819622681044791 1104 6 0.10220980433082616 1104 7 0.1538084024474955 1104 8 0.16054026988197734 1104 9 0.1778455047144547 1104 10-11 0.18664344547885747 1104 12-13 0.17276637729620603 1104 14-15 0.1876769393349349 1104 16-17 0.1380014885390608 1104 18-19 0.12982481642220733 1104 20-21 0.05517895195050926 1104 22-23 0.0648778495549962 1104 24-25 0.030125756533031733 1104 26-27 0.051351171055692646 1104 28-29 0.11213643567769083 1104 30-31 0.1951060633539754 1104 32-33 0.1196070498937658 1104 34-35 0.122078056290718 1104 36-37 0.18499482900467257 1104 38-39 0.14110638378378582 1104 40-41 0.1360807245116078 1104 42-43 0.07068430353442068 1104 44-45 0.0632151804857557 1104 46-47 0.09287383781732927 1104 48-49 -0.0022566422590415414 1104 50-51 0.00290424479119622 1104 52-53 0.04108771013032708 1104 54-55 -0.024963893911099433 1104 56-57 0.010012539493367001 1104 58-59 -0.07343141605402792 1104 60-61 -0.1547965739682553 1104 62-63 -0.14551921724941508 1104 64-65 0.010892744698153933 1104 66-67 0.07169466175008665 1104 68-69 -0.1365774969437723 1104 70-71 -0.12785085247965355 1104 72-73 -0.1391099717378097 1104 74-75 -0.10084297525421704 1104 76-77 0.017008151827621276 1104 78-79 -0.007530175233775083 1104 80-81 0.08459730351329142 1104 82-83 0.0030555236312750367 1104 84-85 -0.16520742363043794 1104 86-87 -0.15118614855810364 1104 88-89 -0.19354986776394867 1104 90-91 -0.09155439289521183 1104 92-93 -0.2710796649313174 1104 94-95 -0.24189366321881423 1104 96-97 -0.41582564041488723 1104 98-99 -0.3560017994081406 1104 100 -0.3272773111902971 1105 1 0.2911155252855977 1105 2 0.165621855351489 1105 3 0.14477528989013422 1105 4 0.08983247232281144 1105 5 0.08649467830874613 1105 6 0.08912889662438062 1105 7 0.09852891546467646 1105 8 0.13169408037968822 1105 9 0.15424510602029784 1105 10-11 0.1842664648161474 1105 12-13 0.193553101491311 1105 14-15 0.21590160860400687 1105 16-17 0.21920370715843518 1105 18-19 0.18923118213998436 1105 20-21 0.11673755958124588 1105 22-23 0.16263442401729122 1105 24-25 0.14185526515048963 1105 26-27 0.18709776723461147 1105 28-29 0.19173991572258586 1105 30-31 0.331106966602718 1105 32-33 0.2918762789139677 1105 34-35 0.2431539840284742 1105 36-37 0.29021696515673767 1105 38-39 0.20140448319462934 1105 40-41 0.21317958243280088 1105 42-43 0.35982597716379416 1105 44-45 0.3326854313402592 1105 46-47 0.2675506892576962 1105 48-49 0.3083456790590091 1105 50-51 0.22695423808709592 1105 52-53 0.2008959903138745 1105 54-55 0.17353448432021423 1105 56-57 0.21096303900280589 1105 58-59 0.21423935563228724 1105 60-61 0.1511829405531131 1105 62-63 0.1962054687067294 1105 64-65 0.2304695293316854 1105 66-67 0.20667170092488618 1105 68-69 0.13568700895203278 1105 70-71 0.12253787822360351 1105 72-73 0.1309330067425023 1105 74-75 0.20598084654581328 1105 76-77 0.14378129957729513 1105 78-79 0.22997769745343177 1105 80-81 0.08834769848945001 1105 82-83 0.10978203345507964 1105 84-85 0.0601662155193452 1105 86-87 0.10701886509699321 1105 88-89 0.03152789301448067 1105 90-91 0.10582819598979398 1105 92-93 0.08489148653713485 1105 94-95 0.04721073285849542 1105 96-97 0.19620501617551867 1105 98-99 0.0972928433392184 1105 100 0.14589192674919715 1106 1 0.23886819245386448 1106 2 0.13866995062348764 1106 3 0.1515439871386306 1106 4 0.10885708406704708 1106 5 0.1164279073627199 1106 6 0.09358915250584943 1106 7 0.1076533319831654 1106 8 0.10682120024264918 1106 9 0.07955236317020109 1106 10-11 0.15701506045187585 1106 12-13 0.16076690119203363 1106 14-15 0.1815320497328159 1106 16-17 0.15439476676206 1106 18-19 0.16690313288477654 1106 20-21 0.14809851841320665 1106 22-23 0.19555065905776559 1106 24-25 0.14787625238309943 1106 26-27 0.2279403245442282 1106 28-29 0.21526884832261572 1106 30-31 0.33275107003375126 1106 32-33 0.28753629593948205 1106 34-35 0.22193017049055896 1106 36-37 0.20747191221915529 1106 38-39 0.19136408123237203 1106 40-41 0.22985692422573578 1106 42-43 0.22664097995999555 1106 44-45 0.2566587215399565 1106 46-47 0.2423374437666581 1106 48-49 0.22270896301222365 1106 50-51 0.19285438748416794 1106 52-53 0.1934893831763489 1106 54-55 0.14740112443031705 1106 56-57 0.21408528101120083 1106 58-59 0.20585065875348363 1106 60-61 0.14866095564462967 1106 62-63 0.18866261972334541 1106 64-65 0.13437868181962642 1106 66-67 0.08787633095465708 1106 68-69 0.19428520791822734 1106 70-71 0.21608312921977557 1106 72-73 0.18692617684921942 1106 74-75 0.11417150670721554 1106 76-77 0.12928267886704603 1106 78-79 0.2274127003174513 1106 80-81 0.1447069373791976 1106 82-83 0.15532978118390872 1106 84-85 0.17784998054450085 1106 86-87 0.1855275280822717 1106 88-89 0.14797167266981504 1106 90-91 0.10829400249940946 1106 92-93 0.20347134419263924 1106 94-95 0.18593771748860632 1106 96-97 0.12197456590285327 1106 98-99 0.11729964052899078 1106 100 0.12746806429819912 1107 1 0.19977438028514172 1107 2 0.1732793143839686 1107 3 0.11435083403726765 1107 4 0.05690269802417447 1107 5 0.06681293369238972 1107 6 0.047784745745360624 1107 7 0.07173406795811843 1107 8 0.0842691980653072 1107 9 0.09952503044574712 1107 10-11 0.114879510403604 1107 12-13 0.1318702213878069 1107 14-15 0.11231850116814002 1107 16-17 0.12758874395253628 1107 18-19 0.14561051155041582 1107 20-21 0.1619834835793199 1107 22-23 0.163001907189809 1107 24-25 0.09766566743366667 1107 26-27 0.13960406577144369 1107 28-29 0.20891358469442167 1107 30-31 0.15576930439642922 1107 32-33 0.16903373041512992 1107 34-35 0.14905857035907388 1107 36-37 0.21400896257976143 1107 38-39 0.23694498338115721 1107 40-41 0.12529783086759494 1107 42-43 0.1975804698218866 1107 44-45 0.27953761067226424 1107 46-47 0.3234521757344666 1107 48-49 0.16182130672089556 1107 50-51 0.16162084900692975 1107 52-53 0.2433994679083682 1107 54-55 0.20411233262554163 1107 56-57 0.20954461096267352 1107 58-59 0.22290084725855053 1107 60-61 0.12333071175997645 1107 62-63 0.19590745630392803 1107 64-65 0.22397847175615482 1107 66-67 0.26250085304612014 1107 68-69 0.15918936663622674 1107 70-71 0.16253126325166534 1107 72-73 0.2173302638847403 1107 74-75 0.19374484622022692 1107 76-77 0.07635491738226108 1107 78-79 0.207544645275469 1107 80-81 0.25135826199163347 1107 82-83 0.191376108531081 1107 84-85 0.1631915709051981 1107 86-87 0.1647593588098708 1107 88-89 0.15954451113893597 1107 90-91 0.07994071954023596 1107 92-93 0.0737379659857531 1107 94-95 0.1994268145065412 1107 96-97 0.1904836824938414 1107 98-99 0.15136050451771865 1107 100 0.37603797468025135 1108 1 0.30354558999390946 1108 2 0.180720494988293 1108 3 0.1446181851000432 1108 4 0.11262837825120897 1108 5 0.09787746931818475 1108 6 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-0.03727478019228414 2316 58-59 -0.0027314889307277213 2316 60-61 0.14824974180145034 2316 62-63 0.2424674293514073 2316 64-65 -0.08333329128329581 2316 66-67 0.01502740614681386 2316 68-69 0.1477715266190387 2316 70-71 -0.1835105074373331 2316 72-73 -0.4220303945115802 2316 74-75 -0.07041080058618121 2316 76-77 -0.0095454948212641 2316 78-79 -0.21447759750166284 2316 80-81 -0.06367137964180003 2316 82-83 0.0306938762634843 2316 84-85 0.14931477974805318 2316 86-87 0.11895363157955785 2316 88-89 0.24933397949179792 2316 90-91 0.22591867016302913 2316 92-93 0.323538810631117 2316 94-95 0.3214565412987156 2316 96-97 0.323674632823888 2316 98-99 0.3698156737458156 2316 100 0.07490066712711041 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 4.0 10 19.0 11 72.0 12 156.0 13 414.0 14 1101.0 15 2958.0 16 6502.0 17 13125.0 18 23289.0 19 36883.0 20 52827.0 21 71764.0 22 93923.0 23 108745.0 24 118374.0 25 130592.0 26 149550.0 27 177256.0 28 211398.0 29 250937.0 30 298520.0 31 355494.0 32 431724.0 33 536362.0 34 695698.0 35 938721.0 36 1353404.0 37 1984271.0 38 1782527.0 39 253910.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.07945488949303 19.89630676125305 13.264128263448532 23.76011008580539 2 15.291673996722224 21.16473623117731 39.554035448744344 23.989554323356117 3 17.36909416870958 26.748149076978873 30.6550866753383 25.227670078973247 4 11.91421684509554 17.119777262674454 38.36837131564501 32.59763457658501 5 12.55701357492996 38.67030722899096 35.49581002677051 13.276869169308567 6 31.39644028643891 38.12113398635818 17.418655114828926 13.063770612373983 7 27.42891205651442 32.81929681955593 22.39890474143156 17.352886382498085 8 26.3863819774187 36.18178328083079 19.85770497690335 17.574129764847157 9 26.84229677332826 14.654598929303603 19.956069822436135 38.547034474932005 10-11 24.917545024917658 26.22019350922174 28.851793732682847 20.01046773317776 12-13 25.37431069176251 23.959381159232237 28.94937361587779 21.716934533127468 14-15 22.637181742667295 25.342889566001986 26.17917400568214 25.84075468564858 16-17 22.33919411888153 28.371624055735555 26.84177416637208 22.44740765901084 18-19 22.229646956213436 27.41369631288217 28.79443725614238 21.562219474762017 20-21 22.91880913121553 26.6194403122459 28.852781814907235 21.608968741631337 22-23 22.788691021943816 26.44314324645025 28.67442759388027 22.093738137725662 24-25 22.46258632970059 26.665530814817462 28.79535719316624 22.07652566231571 26-27 22.475015668837653 26.803900355120643 28.744534755059213 21.976549220982495 28-29 22.448258650376104 26.666080708554308 28.925172438097803 21.96048820297179 30-31 22.663171080914246 26.6747401437403 28.79714533225963 21.86494344308583 32-33 22.513955432198394 26.781850723513866 28.705430821933405 21.998763022354336 34-35 22.68911706300293 26.816454250192876 28.725295802559476 21.76913288424472 36-37 22.76076671788833 26.757261886220444 28.583787103052767 21.898184292838458 38-39 22.602325413094473 26.918114620494045 28.571553993730596 21.908005972680883 40-41 22.616811025701885 26.800204664678056 28.64568878518418 21.937295524435875 42-43 22.48165143804151 26.827048086050727 28.537430907653576 22.15386956825419 44-45 22.48049256705332 27.04977294177937 28.35347459074484 22.11625990042247 46-47 22.53489904385427 27.028576136669656 28.17707007209846 22.259454747377614 48-49 22.561634281117293 27.067188240258577 28.07790448727002 22.293272991354115 50-51 22.562451488018233 27.284954023736546 27.874181149493886 22.27841333875133 52-53 22.615438109518823 27.262044297798106 27.80367126198577 22.318846330697305 54-55 22.510595666355968 27.27085902688075 27.8776132393163 22.340932067446985 56-57 22.57946254232021 27.359910834593855 27.71254473833083 22.348081884755103 58-59 22.57212632439405 27.19359286054234 27.890867524758022 22.343413290305588 60-61 22.660545061370325 27.370274280735686 27.801258945074704 22.16792171281929 62-63 22.49150396362643 27.37693074373629 27.865406949576194 22.26615834306109 64-65 22.481493035591576 27.154017597495738 27.87906271625892 22.48542665065376 66-67 22.442137321184155 27.30190143137004 27.72669873658058 22.529262510865227 68-69 22.41916675269506 27.328306629309264 27.791664411573468 22.46086220642221 70-71 22.420735110557853 27.207203685850345 27.778787471743726 22.59327373184808 72-73 22.4519846611253 27.408009562638085 27.52566739493019 22.614338381306425 74-75 22.4394822519534 27.504916054786786 27.485094093303502 22.570507599956308 76-77 22.351801245982422 27.38362242831851 27.61294605061588 22.65163027508319 78-79 22.382708211865054 27.355059207895437 27.568266268621944 22.69396631161756 80-81 22.302513316045708 27.61562287059714 27.356323389915353 22.725540423441796 82-83 22.437089459490313 27.581987306397863 27.383495678074475 22.59742755603735 84-85 22.329135185769232 27.557220281469725 27.33849926903642 22.775145263724625 86-87 22.275910620177726 27.48970216763338 27.52720114508056 22.70718606710834 88-89 22.20097873707933 27.48236490325786 27.476416837659084 22.840239522003724 90-91 22.31107214169344 27.550122461924275 27.4319523115941 22.706853084788186 92-93 22.284259529510372 27.535624236988497 27.368806673456653 22.811309560044474 94-95 22.14618639295488 27.38213192301826 27.428318702971623 23.043362981055235 96-97 22.178795042270146 27.37295453856557 27.475051185990473 22.973199233173812 98-99 22.058769177939038 27.380155972939264 27.379992261926112 23.181082587195586 100 21.183729190663495 27.79684735631148 27.591967486718627 23.427455966306397 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2600.0 1 1928.5 2 2158.5 3 3160.5 4 2495.5 5 2155.5 6 3439.0 7 6858.5 8 11117.0 9 13209.5 10 11554.5 11 9386.0 12 9525.5 13 10150.5 14 11009.0 15 11764.0 16 12528.5 17 13343.0 18 14236.5 19 15350.0 20 16913.0 21 19588.5 22 23196.0 23 28218.0 24 35313.0 25 44441.5 26 55555.0 27 67359.0 28 80037.0 29 95225.5 30 113814.5 31 132205.0 32 152789.0 33 177022.5 34 203007.0 35 225689.5 36 252736.0 37 278705.5 38 294381.0 39 308679.0 40 312958.5 41 315768.5 42 322939.0 43 331611.5 44 342216.5 45 355280.5 46 363939.5 47 366323.5 48 373116.5 49 377956.0 50 376812.0 51 368395.0 52 355612.0 53 346922.0 54 335440.5 55 314092.0 56 285673.5 57 256291.0 58 229489.0 59 200166.5 60 168788.0 61 140224.0 62 114406.5 63 88966.5 64 66469.0 65 49755.0 66 36521.5 67 27094.0 68 20585.0 69 15440.0 70 11844.0 71 9087.0 72 6912.5 73 5611.5 74 4508.5 75 3396.0 76 2518.5 77 1716.0 78 1168.0 79 841.5 80 584.5 81 424.5 82 334.0 83 231.5 84 163.5 85 125.5 86 89.5 87 65.5 88 44.0 89 24.0 90 19.5 91 12.0 92 4.5 93 4.0 94 3.5 95 3.5 96 2.0 97 1.5 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24912350943034609 2 0.07795215773120136 3 0.001646736852046249 4 0.0014681750247159328 5 4.7616487288084313E-4 6 6.745669032478611E-4 7 0.0 8 7.638478169130192E-4 9 0.0 10-11 4.017641114932114E-4 12-13 0.001448334821679231 14-15 4.960050759175449E-6 16-17 1.0416106594268444E-4 18-19 0.0012003322837204585 20-21 0.001522735583066863 22-23 2.4800253795877245E-5 24-25 1.9840203036701797E-5 26-27 2.3808243644042157E-4 28-29 0.0018401788316540916 30-31 0.0020782612680945133 32-33 0.003248833247259919 34-35 0.00419620294226243 36-37 0.0017062574611563545 38-39 0.003090111622966305 40-41 0.006343904920985399 42-43 0.012677889740452447 44-45 0.008516407153504246 46-47 0.006403425530095505 48-49 0.006552227052870768 50-51 0.005535416647239801 52-53 0.0035414762420512705 54-55 0.00590742045417796 56-57 0.003963080556581184 58-59 0.0022270627908697765 60-61 0.008263444564786298 62-63 0.01126923532484662 64-65 0.007494636697114104 66-67 0.007871600554811438 68-69 0.014934712835877278 70-71 0.004761648728808431 72-73 0.01304493349663143 74-75 0.008065042534419281 76-77 0.004602927104514817 78-79 0.01594656319074907 80-81 0.006859750199939646 82-83 0.015152955069280997 84-85 0.023872724303911436 86-87 0.01587712248012061 88-89 0.016219365982503717 90-91 0.030990397143328206 92-93 0.024467930395012494 94-95 0.029591662829240726 96-97 0.018416668468818443 98-99 0.0179206633929009 100 0.019373958265339303 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.0080542E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.01700884024084 #Duplication Level Percentage of deduplicated Percentage of total 1 66.87588842059455 33.449319023318374 2 17.729554051284392 17.735585234332383 3 6.749842438792592 10.128207867939656 4 3.1722195755361877 6.346597382111143 5 1.744837966800112 4.363578800511453 6 1.003453229414797 3.0113837427844876 7 0.6382055317875389 2.234479220771556 8 0.43228139450383907 1.7297137864296124 9 0.30861939003379807 1.3892596883631192 >10 1.2468922265561424 10.429962313150975 >50 0.05790285965773559 2.0012316736445035 >100 0.03565852729499047 3.4378247108618214 >500 0.0030304006889703785 1.0124453721782316 >1k 0.0014822330092555253 1.2190241762381107 >5k 3.2938511285276793E-5 0.08700372563349668 >10k+ 9.881553385583037E-5 1.4243832817308948 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34690 0.3441283216715927 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30578 0.3033368642281338 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21190 0.21020695117385554 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 9.920101518350899E-6 9.920101518350899E-6 4.960050759175449E-6 16-17 0.0 0.0 9.920101518350899E-6 9.920101518350899E-6 1.9840203036701797E-5 18-19 0.0 0.0 9.920101518350899E-6 9.920101518350899E-6 2.9760304555052696E-5 20-21 0.0 0.0 9.920101518350899E-6 3.4720355314228147E-5 2.9760304555052696E-5 22-23 0.0 0.0 9.920101518350899E-6 7.440076138763174E-5 3.4720355314228147E-5 24-25 0.0 0.0 9.920101518350899E-6 1.5872162429361438E-4 3.9680406073403594E-5 26-27 0.0 0.0 9.920101518350899E-6 3.0752314706887783E-4 3.9680406073403594E-5 28-29 0.0 0.0 9.920101518350899E-6 0.0013540938572548975 3.9680406073403594E-5 30-31 0.0 9.920101518350899E-6 9.920101518350899E-6 0.00457812685071894 3.9680406073403594E-5 32-33 0.0 1.9840203036701797E-5 9.920101518350899E-6 0.010629388776912989 4.464045683257904E-5 34-35 0.0 1.9840203036701797E-5 9.920101518350899E-6 0.01853074963627948 4.960050759175449E-5 36-37 0.0 1.9840203036701797E-5 9.920101518350899E-6 0.032200649528567014 4.960050759175449E-5 38-39 0.0 1.9840203036701797E-5 9.920101518350899E-6 0.05369254946807424 4.960050759175449E-5 40-41 0.0 1.9840203036701797E-5 9.920101518350899E-6 0.07898384828910984 4.960050759175449E-5 42-43 0.0 1.9840203036701797E-5 9.920101518350899E-6 0.10191416294877795 4.960050759175449E-5 44-45 0.0 1.9840203036701797E-5 9.920101518350899E-6 0.12838595385049734 4.960050759175449E-5 46-47 0.0 1.9840203036701797E-5 9.920101518350899E-6 0.1606560440896928 4.960050759175449E-5 48-49 0.0 1.9840203036701797E-5 9.920101518350899E-6 0.1965172110785313 4.960050759175449E-5 50-51 0.0 1.9840203036701797E-5 9.920101518350899E-6 0.23525520750769158 4.960050759175449E-5 52-53 0.0 1.9840203036701797E-5 9.920101518350899E-6 0.27351703906397096 6.448065986928083E-5 54-55 0.0 1.9840203036701797E-5 9.920101518350899E-6 0.31418945528920966 8.432086290598264E-5 56-57 0.0 1.9840203036701797E-5 9.920101518350899E-6 0.3617563420697022 8.928091366515808E-5 58-59 0.0 1.9840203036701797E-5 9.920101518350899E-6 0.4150173671217282 8.928091366515808E-5 60-61 0.0 1.9840203036701797E-5 9.920101518350899E-6 0.471894269177193 8.928091366515808E-5 62-63 0.0 1.9840203036701797E-5 9.920101518350899E-6 0.527585719101215 8.928091366515808E-5 64-65 0.0 1.9840203036701797E-5 9.920101518350899E-6 0.5835698120200283 8.928091366515808E-5 66-67 0.0 1.9840203036701797E-5 9.920101518350899E-6 0.6472767039708778 9.424096442433353E-5 68-69 0.0 1.9840203036701797E-5 9.920101518350899E-6 0.7205812941407317 9.920101518350898E-5 70-71 0.0 1.9840203036701797E-5 9.920101518350899E-6 0.7998627454753922 1.0912111670185987E-4 72-73 0.0 1.9840203036701797E-5 9.920101518350899E-6 0.8802155677740344 1.2896131973856166E-4 74-75 0.0 1.9840203036701797E-5 9.920101518350899E-6 0.9571707553026415 1.2896131973856166E-4 76-77 0.0 1.9840203036701797E-5 9.920101518350899E-6 1.0400730436915 1.2896131973856166E-4 78-79 0.0 1.9840203036701797E-5 9.920101518350899E-6 1.136511310602148 1.2896131973856166E-4 80-81 0.0 1.9840203036701797E-5 9.920101518350899E-6 1.242438154615099 1.3392137049773713E-4 82-83 0.0 1.9840203036701797E-5 9.920101518350899E-6 1.3528439244635853 1.4384147201608802E-4 84-85 0.0 1.9840203036701797E-5 9.920101518350899E-6 1.4575456359390198 1.4880152277526348E-4 86-87 0.0 1.9840203036701797E-5 9.920101518350899E-6 1.564350408936345 1.5872162429361438E-4 88 0.0 1.9840203036701797E-5 9.920101518350899E-6 1.6511016967143235 1.5872162429361438E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15280 0.0 51.773735 1 GTATCAA 27485 0.0 39.743965 1 TCAACGC 34085 0.0 31.197884 4 ATCAACG 34580 0.0 30.792976 3 CAACGCA 34705 0.0 30.613302 5 AACGCAG 36020 0.0 29.613981 6 GTACATG 35165 0.0 29.522125 1 TATCAAC 36565 0.0 29.416006 2 TACATGG 36435 0.0 28.07525 2 ACATGGG 36960 0.0 26.635988 3 ACGCAGA 41255 0.0 25.628345 7 CGCAGAG 42725 0.0 24.658585 8 CATGGGG 24110 0.0 24.635065 4 GTACCTG 8245 0.0 23.386948 1 TACCTGG 8395 0.0 21.792751 2 GCAGAGT 47990 0.0 21.59088 9 ATGGGGG 13720 0.0 21.131617 5 AGAGTAC 43785 0.0 20.428665 10-11 GAGTACT 30205 0.0 19.77286 12-13 AGTACTT 32120 0.0 18.87927 12-13 >>END_MODULE