FastQCFastQC Report
Mon 6 Jun 2016
ERR1211653_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211653_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6727818
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198000.2943004700781145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166510.24749480440761032No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111940.16638381121486936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93650.050.1584361
GTATCAA164200.039.4931641
TCAACGC201850.031.4234774
CAACGCA203550.031.1118645
TACCTGG59050.030.8354932
ATCAACG205550.030.8342933
GTACCTG60050.030.7868651
AACGCAG212900.029.8328916
TATCAAC213500.029.7285632
GTACATG204700.029.3293861
TACATGG211550.027.711812
ACATGGG210900.026.8218943
ACGCAGA240900.026.112467
CGCAGAG247350.025.2041878
CATGGGG146350.024.3988914
ACCTGGG76350.022.5222423
GCAGAGT278900.021.662389
TATAACG5950.021.3504312
TAACGCA6500.020.9620554
ATGGGGG88900.020.3453985
Produced by FastQC (version 0.11.5)